ALS Antibody (E-2): sc-377131

ALS Antibody (E-2) is a mouse monoclonal IgG1 kappa light chain antibody that detects ALS protein of human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). Anti-ALS antibody (E-2) is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. ALS protein, also known as insulin-like growth factor binding protein acid-labile subunit, plays a crucial role in regulating insulin-like growth factor (IGF) signaling. ALS protein is primarily found in plasma, where ALS protein interacts with IGF binding proteins to form stable complexes that enhance bioavailability and half-life of IGFs in circulation. Such interaction is vital for maintaining proper cellular functions and growth regulation, as IGFs are key players in cell proliferation, differentiation, and survival. Through stabilizing these complexes, ALS protein contributes to fine-tuning IGF activity, which is essential for normal physiological processes and has implications in various pathologies, including cancer and metabolic disorders.

Alexa Fluor^® is a trademark of Molecular Probes Inc., OR., USA

LI-COR^® and Odyssey^® are registered trademarks of LI-COR Biosciences

ALS Antibody (E-2) References:

1. Structure and functional expression of the acid-labile subunit of the insulin-like growth factor-binding protein complex.  |  Leong, SR., et al. 1992. Mol Endocrinol. 6: 870-6. PMID: 1379671
2. The role of the acid-labile subunit in regulating insulin-like growth factor transport across human umbilical vein endothelial cell monolayers.  |  Payet, LD., et al. 2004. J Clin Endocrinol Metab. 89: 2382-9. PMID: 15126567
3. Plasma insulin-like growth factors (IGFs), IGF-Binding proteins (IGFBPs), acid-labile subunit (ALS) and IGFBP-3 proteolysis in individuals with clinical characteristics of Sotos syndrome.  |  de Boer, L., et al. 2004. J Pediatr Endocrinol Metab. 17: 615-27. PMID: 15198293
4. Associations of insulin-like growth factors, insulin-like growth factor binding proteins and acid-labile subunit with coronary heart disease.  |  Fischer, F., et al. 2004. Clin Endocrinol (Oxf). 61: 595-602. PMID: 15521962
5. Expression of porcine acid-labile subunit (pALS) of the 150-kilodalton ternary insulin-like growth factor complex and initial characterization of recombinant pALS protein.  |  Lee, DH., et al. 2005. J Biochem Mol Biol. 38: 225-31. PMID: 15826501
6. Human plasma N-glycoproteome analysis by immunoaffinity subtraction, hydrazide chemistry, and mass spectrometry.  |  Liu, T., et al. 2005. J Proteome Res. 4: 2070-80. PMID: 16335952
7. High molecular weight insulin-like growth factor binding protein complex. Purification and properties of the acid-labile subunit from human serum.  |  Baxter, RC., et al. 1989. J Biol Chem. 264: 11843-8. PMID: 2473065

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-ALS Antibody (E-2) has 2 citations in a variety of scientific publications.

Primary antibodies like Anti-ALS Antibody (E-2) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

ALS Antibody (E-2) Specifications

Alternate Product Name: ALS

Product Clone Name: E-2

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG₁ kappa light chain

Amino Acids: 304-345

Epitope Target: ALS

Epitope Species: human

Product Reactivity/Detection: ALS

Species Reactivity/Detection: human

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, ELISA

Vialing Information: Each vial contains 200 µg IgG₁ kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 2 citations in various scientific publications

Available Conjugates/Forms for ALS Antibody (E-2): AC: Agarose, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, FITC: Fluoroscein, HRP: Horseradish Peroxidase, PE: Phycoerythrin