αENaC Antibody (H-6): sc-518119
- αENaC Antibody (H-6) is a mouse monoclonal IgG1 κ provided at 200 µg/ml
- specific for an epitope mapping between amino acids 206-229 of αENaC of human origin
- recommended for detection of αENaC of human origin by WB, IP, IF and ELISA
- Anti-alpha ENaC Antibody (H-6) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
- also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- m-IgGκ BP-HRP is the preferred secondary detection reagent for αENaC Antibody (H-6) for WB applications. This reagent is now offered in a bundle with αENaC Antibody (H-6) (see ordering information below). For additional m-IgGκ BP conjugates see our complete list of Mouse IgG Binding Proteins.
QUICK LINKS
αENaC (H-6) is a mouse monoclonal IgG1 kappa light chain antibody that detects αENaC of human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF) and enzyme-linked immunosorbent assay (ELISA). anti-αENaC antibody (H-6) is available as non-conjugated form, as well as multiple conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC) and multiple Alexa Fluor® conjugates. The epithelial sodium channel (ENaC) is a member of the ENaC/DEG superfamily located on the apical surface of cells. ENaC mediates sodium reabsorption in kidney, distal colon, lung, ducts of exocrine glands, and other organs. ENaC forms through heteromultimerization of four homologous subunits, α, β, γ and δ. The most frequently formed heterotetramer consists of two α, one β, and one γ subunit, though α subunit can be replaced by a δ subunit. The αENaC gene maps to human chromosome 12p13. Both β and γENaC genes map to human chromosome 16p12, and γENaC transcript is detected as a glycosylated protein. The carboxy terminus of all ENaC subunits contains PY motifs, which interact with ubiquitin protein ligase, Nedd4, to regulate intracellular sodium concentrations. Gain-of-function mutations involving PY motif cause Liddle′s syndrome, an autosomal dominant form of hypertension, resulting from excessive renal sodium absorption. Conversely, ENaC loss-of-function mutations result in pseudohypoaldosteronism type I, a disorder characterized by salt wasting and hypotension.
Alexa Fluor® is a trademark of Molecular Probes Inc., OR., USA
LI-COR® and Odyssey® are registered trademarks of LI-COR Biosciences
αENaC Antibody (H-6) References:
- Aldosterone-mediated regulation of ENaC alpha, beta, and gamma subunit proteins in rat kidney. | Masilamani, S., et al. 1999. J Clin Invest. 104: R19-23. PMID: 10510339
- Trafficking and cell surface stability of the epithelial Na+ channel expressed in epithelial Madin-Darby canine kidney cells. | Hanwell, D., et al. 2002. J Biol Chem. 277: 9772-9. PMID: 11773057
- Liddle syndrome: genetics and mechanisms of Na+ channel defects. | Warnock, DG. 2001. Am J Med Sci. 322: 302-7. PMID: 11780687
- Rabbit retinal neurons and glia express a variety of ENaC/DEG subunits. | Brockway, LM., et al. 2002. Am J Physiol Cell Physiol. 283: C126-34. PMID: 12055080
- Cloning, chromosomal localization, and physical linkage of the beta and gamma subunits (SCNN1B and SCNN1G) of the human epithelial amiloride-sensitive sodium channel. | Voilley, N., et al. 1995. Genomics. 28: 560-5. PMID: 7490094
- Cloning, expression, and tissue distribution of a human amiloride-sensitive Na+ channel. | McDonald, FJ., et al. 1994. Am J Physiol. 266: L728-34. PMID: 8023962
- Structural organisation of the gene encoding the alpha-subunit of the human amiloride-sensitive epithelial sodium channel. | Ludwig, M., et al. 1998. Hum Genet. 102: 576-81. PMID: 9654208
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
αENaC Antibody (H-6) | sc-518119 | 200 µg/ml | $322.00 | |||
αENaC Antibody (H-6): m-IgGκ BP-HRP Bundle | sc-525384 | 200 µg Ab, 40 µg BP | $361.00 | |||
αENaC Antibody (H-6) AC | sc-518119 AC | 500 µg/ml, 25% agarose | $424.00 | |||
αENaC Antibody (H-6) HRP | sc-518119 HRP | 200 µg/ml | $322.00 | |||
αENaC Antibody (H-6) FITC | sc-518119 FITC | 200 µg/ml | $336.00 | |||
αENaC Antibody (H-6) PE | sc-518119 PE | 200 µg/ml | $349.00 | |||
αENaC Antibody (H-6) Alexa Fluor® 488 | sc-518119 AF488 | 200 µg/ml | $364.00 | |||
αENaC Antibody (H-6) Alexa Fluor® 546 | sc-518119 AF546 | 200 µg/ml | $364.00 | |||
αENaC Antibody (H-6) Alexa Fluor® 594 | sc-518119 AF594 | 200 µg/ml | $364.00 | |||
αENaC Antibody (H-6) Alexa Fluor® 647 | sc-518119 AF647 | 200 µg/ml | $364.00 | |||
αENaC Antibody (H-6) Alexa Fluor® 680 | sc-518119 AF680 | 200 µg/ml | $364.00 | |||
αENaC Antibody (H-6) Alexa Fluor® 790 | sc-518119 AF790 | 200 µg/ml | $364.00 |