αA-crystallin Antibody (SUB4): sc-69687
- αA-crystallin Antibody (SUB4) is a mouse monoclonal IgG1 κ provided at 200 µg/ml
- raised against full length αA-crystallin of bovine origin
- recommended for detection of αA-crystallin of mouse, rat, human, bovine and avian origin by WB, IP, IF and ELISA
- m-IgG Fc BP-HRP is the preferred secondary detection reagent for αA-crystallin Antibody (SUB4) for WB applications. This reagent is now offered in a bundle with αA-crystallin Antibody (SUB4) (see ordering information below).
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αA-crystallin Antibody (SUB4) is a mouse monoclonal IgG1 antibody that detects αA-crystallin in mouse, rat, human, bovine, and avian species through western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). αA-crystallin (SUB4) antibody recognizes a crucial member of the small heat shock protein family, specifically involved in maintaining lens transparency and refractive index, which is vital for proper vision. αA-crystallin functions as a molecular chaperone, preventing the aggregation of denatured proteins, although not facilitating their renaturation. The unique structure of αA-crystallin, which includes seven distinct protein regions and four homologous motifs, allows formation of large soluble aggregates that stabilize other proteins under stress conditions. Expression of αA-crystallin remains restricted to the lens, and mutations in the αA-crystallin gene can lead to autosomal dominant congenital cataracts, highlighting its importance in ocular health. Anti-αA-crystallin antibody (SUB4) serves as an essential tool for researchers studying lens biology, protein aggregation, and related pathologies, providing reliable detection and analysis across various experimental applications.
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αA-crystallin Antibody (SUB4) References:
- AlphaA-crystallin interacting regions in the small heat shock protein, alphaB-crystallin. | Sreelakshmi, Y., et al. 2004. Biochemistry. 43: 15785-95. PMID: 15595834
- αA-Crystallin associates with α6 integrin receptor complexes and regulates cellular signaling. | Menko, AS. and Andley, UP. 2010. Exp Eye Res. 91: 640-51. PMID: 20709056
- The N-terminal extension of βB1-crystallin chaperones β-crystallin folding and cooperates with αA-crystallin. | Leng, XY., et al. 2014. Biochemistry. 53: 2464-73. PMID: 24669963
- In vivo substrates of the lens molecular chaperones αA-crystallin and αB-crystallin. | Andley, UP., et al. 2014. PLoS One. 9: e95507. PMID: 24760011
- The small heat shock protein αA-crystallin negatively regulates pancreatic tumorigenesis. | Liu, J., et al. 2016. Oncotarget. 7: 65808-65824. PMID: 27588467
- RNA aptamers targeted for human αA-crystallin do not bind αB-crystallin, and spare the α-crystallin domain. | Mallik, PK., et al. 2017. Biochem Biophys Res Commun. 491: 423-428. PMID: 28720498
- Asp 58 modulates lens αA-crystallin oligomer formation and chaperone function. | Takata, T., et al. 2018. FEBS J. 285: 2263-2277. PMID: 29676852
- αA-crystallin-derived minichaperone stabilizes αAG98R-crystallin by affecting its zeta potential. | Phadte, AS., et al. 2018. Mol Vis. 24: 297-304. PMID: 29706763
- Characterization of an N-terminal mutant of αA-crystallin αA-R21Q associated with congenital cataract. | Phadte, AS., et al. 2018. Exp Eye Res. 174: 185-195. PMID: 29782825
- The structure and oxidation of the eye lens chaperone αA-crystallin. | Kaiser, CJO., et al. 2019. Nat Struct Mol Biol. 26: 1141-1150. PMID: 31792453
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
αA-crystallin Antibody (SUB4) | sc-69687 | 200 µg/ml | $316.00 | |||
αA-crystallin Antibody (SUB4): m-IgG Fc BP-HRP Bundle | sc-539222 | 200 µg Ab; 10 µg BP | $354.00 |