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alpha Tubulin Antibody (4G1): sc-58666

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Datasheets
  • alpha Tubulin Antibody (4G1) is a mouse monoclonal IgG1, cited in 52 publications, provided at 100 µg/ml
  • raised against the C-terminus of α Tubulin of human origin
  • recommended for detection of α Tubulin of mouse, rat and human origin by WB, IP, IF and IHC(P)
  • See alpha Tubulin (TU-02): sc-8035 for alpha Tubulin antibody conjugates, including AC, HRP, FITC, PE, Alexa Fluor® 488, 594, 647, 680 and 790.
  • At present, we have not yet completed the identification of the preferred secondary detection reagent(s) for alpha Tubulin Antibody (4G1). This work is in progress.
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α Tubulin Antibody (4G1) is a mouse monoclonal IgG1 antibody that recognizes α Tubulin, a crucial component of the cytoskeleton, in mouse, rat, and human samples. Anti-α Tubulin antibody (4G1) is raised against the C-terminal region of α Tubulin from human origin and is suitable for use in various applications, including western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and immunohistochemistry with paraffin-embedded sections (IHCP). α Tubulin plays a pivotal role in maintaining cellular structure and function by forming part of the microtubule network, where it forms heterodimers with β Tubulin to assemble microtubules that are involved in processes such as mitosis, intracellular transport, and cell shape. α Tubulin exists in multiple isoforms and undergoes important post-translational modifications, such as tyrosination, detyrosination, and acetylation, which regulate its dynamics, stability, and interactions with other proteins. These modifications are crucial for controlling the polymerization and stability of microtubules, which are essential for cellular processes like cell division and motility. α Tubulin monoclonal antibody (4G1) targets the tyrosinated form of α Tubulin, which is particularly relevant for understanding the dynamic regulation of microtubules during cellular activities. Anti-α Tubulin antibody (4G1) is available in a non-conjugated form, providing flexibility for a range of experimental setups. Given the importance of microtubules in diverse cellular functions, α Tubulin (4G1) antibody is an invaluable tool for studying cytoskeletal dynamics and the role of α Tubulin in cellular processes.

For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.

Ordering Information

Product NameCatalog #UNITPriceQtyFAVORITES

alpha Tubulin Antibody (4G1)

sc-58666
100 µg/ml
$322.00

what a-tubulin isoforms being detecgted by sc-58666 antibody ? a1, a3, a4?

Asked by: Anonymous
Thank you for your question. This monoclonal antibody is specific for alpha tubulin. The specific isoform reactivity has not been determined.
Answered by: Technical Support
Date published: 2019-07-26

Is α Tubulin (4G1): sc-58666 monoclonal antibody available directly conjugated to HRP or fluorescent dyes?

Asked by: jenni
Thank you for your question. All requests for directly conjugated versions of this antibody should be directed to our Special Orders team at specialorders@scbt.com Alternatively, consider using α Tubulin (TU-02): sc-8035, which is available conjugated to agarose, HRP, FITC, PE, and more. (https://www.scbt.com/scbt/product/alpha-tubulin-antibody-tu-02)
Answered by: Technical Support 15
Date published: 2017-01-10
  • y_2026, m_2, d_11, h_6CST
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Rated 5 out of 5 by from Good performanceThis is a excellent antibody for tubulin detection.It gave strong clean band at 55kDa, without background.We test mouse and rat samples,both great restult.
Date published: 2018-05-26
Rated 4 out of 5 by from Clean westernStrong western,low background; not as good as alternate alpha tubulin monoclonals available from santa cruz
Date published: 2017-01-14
Rated 5 out of 5 by from Good antibody for Western blotThe alpha-Tubulin Antibody (4G1) is a good antibody for Western blot applications showing strong signal with minimal background and over 10 publication citations for verification.
Date published: 2016-12-28
Rated 3 out of 5 by from Star with high concentration of antibodyStar with high concentration of antibody to get decent signal, data in SH-SY5Y whole cell lysate and mouse brain and rat brain tissue extracts. -SCBT QC
Date published: 2015-02-04
Rated 4 out of 5 by from Reasonably specific and sensitive for WBReasonably specific and sensitive for WB in NIH/3T3, HeLa, K-562 and PC-12 whole cell lysates. -SCBT QC
Date published: 2014-12-14
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alpha Tubulin Antibody (4G1) is rated 4.2 out of 5 by 5.
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