alpha-synuclein CRISPR Plasmids (h) human α-synuclein-specific CRISPR/Cas9 KO Plasmid, HDR Plasmid, Double Nickase Plasmids, CRISPR Activation Plasmids and Lentiviral Activation Particles

α-synuclein CRISPR Plasmids (h)

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Datasheets

    • Target species: human; for corresponding mouse product, see α-synuclein CRISPR Plasmids (m)
    • CRISPR/Cas9 KO Plasmids consists of α-synuclein-specific 20 nt guide RNA sequences derived from the GeCKO (v2) library
    • For CRISPR gene knockout, gRNA sequences direct the Cas9 protein to induce a site-specific double strand break (DSB) in the genomic DNA
    • Target-specific CRISPR Plasmids for both gene knockout and activation are available. Please refer to the detailed product information in the tabs below
    • Gene knockdown or activation can be assayed using α-synuclein Antibody (211): sc-12767
    • All products are provided as transfection-ready, purified plasmid DNA, except the Lentiviral Activation Particles which have been prepackaged as Lentiviral Particles for use with hard-to-transfect cells
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Gene Info

SpeciesGene NameGene IDChromosome LocationIsoform (mRNA) Accession #Protein Accession #OMIM™ Number
HumanSNCA66224q22.1XM_011532203, XM_011532204, XM_011532205, XM_011532206, XM_011532207, XM_011532208, XM_017008562, XM_017008563, NM_000345, NM_001146054, NM_001146055, NM_007308P37840605543

Does GFP stay in cells after the KO with the HDR plasmid co-transfected? Will the cells have both GFP and RFP stably expressed or just RFP?

Asked by: Chanat
Thank you for your question. The knockout plasmid, which contains the GFP gene, is not stably integrated into the cell's genome. GFP only serves as an early transfection control. The part of the HDR plasmid that is integrated into the genome through homology-directed repair contains a puomycin resistance gene alongside two LoxP sites. You can find more information on this process on our CRISPR Systems page: https://www.scbt.com/scbt/whats-new/crispr-systems?&_requestid=22452638
Answered by: Tech Support Europe
Date published: 2019-06-16
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