alpha-synuclein Antibody (LB 509) recommended for detection of α-synuclein of human origin by WB, IP, IF and IHC(P)

α-synuclein Antibody (LB 509): sc-58480

α-synuclein Antibody (LB 509) is rated 3.8 out of 5 by 5.
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Datasheets

    • α-synuclein Antibody (LB 509) is a mouse monoclonal IgG1 (kappa light chain) provided at 200 µg/ml
    • raised against Lewy bodies of human origin
    • alpha-synuclein Antibody (LB 509) is recommended for detection of α-synuclein of human origin by WB, IP, IF and IHC(P)
    • Anti-alpha-synuclein Antibody (LB 509) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
    • also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
    • also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
    • See m-IgGκ BP-HRP (mouse IgGκ binding protein-HRP), our highly recommended recombinant alternative to conventional secondary anti-mouse IgG reagents.
    • Contact our Technical Service Department (or your local Distributor) for more information on how to receive a FREE 10 µg sample of α-synuclein (LB 509): sc-58480.
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Can α-synuclein Antibody (LB 509): sc-58480 be used to stain formalin-fixed, paraffin-embedded (FFPE) tissue sections?

Asked by: jenni
Thank you for your question. Yes, α-synuclein Antibody (LB 509): sc-58480 has been validated for use in IHC with paraffin-embedded sections. We recommend performing antigen retrieval with sodium citrate buffer (pH 6) and heat. The full protocol can be found here: https://www.scbt.com/scbt/resources/protocols/immunoperoxidase-staining
Answered by: Technical Support 10
Date published: 2017-01-09
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Rated 1 out of 5 by from non-specific for IHC showed non-specific IHC staining with 1:100 dilution
Date published: 2018-02-13
Rated 5 out of 5 by from Excellent Strong western blot and IHC data; very nice antibody
Date published: 2017-01-07
Rated 5 out of 5 by from Works for IHC(P) IHC(P) data in human brain tissue specific and strong.
Date published: 2016-12-29
Rated 4 out of 5 by from Works well for WB with human Works well for WB with human -synuclein transfected 293T whole cell lysate. -SCBT QC
Date published: 2015-01-16
Rated 4 out of 5 by from Very strong signal in WB on human brain and Very strong signal in WB on human brain and human recombinant -synuclein.
Date published: 2013-04-23
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