α-internexin Antibody (2E3): sc-58478

α-internexin Antibody (2E3) is a mouse monoclonal IgG1 antibody that detects α-internexin in mouse, rat, and human samples through various applications including western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), immunohistochemistry with paraffin-embedded sections (IHCP), and enzyme-linked immunosorbent assay (ELISA). α-internexin monoclonal antibody (2E3) is available in a non-conjugated form, providing flexibility for experimental design. α-internexin is a brain-specific type IV intermediate filament protein predominantly found in the central nervous system, where α-internexin plays a crucial role in maintaining neuronal structure and function. This protein is essential for the formation of a filamentous network that supports axonal integrity, particularly during the critical stages of brain development. The head domain of α-internexin is vital for self-assembly, which is necessary for the stability of neuronal cells. Notably, α-internexin expression peaks during late embryogenesis and decreases into adulthood, indicating significant involvement in the regulatory processes that govern brain maturation. Furthermore, α-internexin expression is intricately controlled by transcription factors such as Brn-3a and Brn-3c, which activate its promoter, while Brn-3b serves to repress it, highlighting the complexity of gene regulation in neuronal development.

Alexa Fluor^® is a trademark of Molecular Probes Inc., OR., USA

LI-COR^® and Odyssey^® are registered trademarks of LI-COR Biosciences

α-internexin Antibody (2E3) References:

1. Axonally transported peripheral signals regulate alpha-internexin expression in regenerating motoneurons.  |  McGraw, TS., et al. 2002. J Neurosci. 22: 4955-63. PMID: 12077192
2. The expression of alpha-internexin and peripherin in the developing mouse pineal gland.  |  Ko, TL., et al. 2005. J Biomed Sci. 12: 777-89. PMID: 16132113
3. Neural expression of alpha-internexin promoter in vitro and in vivo.  |  Wang, P., et al. 2006. J Cell Biochem. 97: 275-87. PMID: 16173078
4. Alpha-internexin is structurally and functionally associated with the neurofilament triplet proteins in the mature CNS.  |  Yuan, A., et al. 2006. J Neurosci. 26: 10006-19. PMID: 17005864
5. The predicted amino acid sequence of alpha-internexin is that of a novel neuronal intermediate filament protein.  |  Fliegner, KH., et al. 1990. EMBO J. 9: 749-55. PMID: 2311576
6. α-Internexin: a novel biomarker for pancreatic neuroendocrine tumor aggressiveness.  |  Liu, B., et al. 2014. J Clin Endocrinol Metab. 99: E786-95. PMID: 24483152
7. Loss of expression and prognosis value of alpha-internexin in gastroenteropancreatic neuroendocrine neoplasm.  |  Wang, Y., et al. 2018. BMC Cancer. 18: 691. PMID: 29940892
8. Epigenetic Inactivation of α-Internexin Accelerates Microtubule Polymerization in Colorectal Cancer.  |  Li, Y., et al. 2020. Cancer Res. 80: 5203-5215. PMID: 33051252
9. Activation of the alpha-internexin promoter by the Brn-3a transcription factor is dependent on the N-terminal region of the protein.  |  Budhram-Mahadeo, V., et al. 1995. J Biol Chem. 270: 2853-8. PMID: 7852360
10. Expression of the gene for the neuronal intermediate filament protein alpha-internexin coincides with the onset of neuronal differentiation in the developing rat nervous system.  |  Fliegner, KH., et al. 1994. J Comp Neurol. 342: 161-73. PMID: 8201029
11. Excitable membranes and synaptic transmission: postsynaptic mechanisms. Localization of alpha-internexin in the postsynaptic density of the rat brain.  |  Suzuki, T., et al. 1997. Brain Res. 765: 74-80. PMID: 9310396
12. Roles of head and tail domains in alpha-internexin's self-assembly and coassembly with the neurofilament triplet proteins.  |  Ching, GY. and Liem, RK. 1998. J Cell Sci. 111 (Pt 3): 321-33. PMID: 9427681

The preferred α-internexin antibody is α-internexin Antibody (G-9): sc-271302.