Date published: 2025-9-13

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α Enolase Antibody (9): sc-101513

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Datasheets
  • α Enolase Antibody (9) is a mouse monoclonal IgG1 κ α Enolase antibody, cited in 2 publications, provided at 200 µg/ml
  • raised against recombinant α Enolase of human origin
  • alpha Enolase Antibody (9) is recommended for detection of α Enolase of mouse, rat and human origin by WB, IP and ELISA
  • Anti-alpha Enolase Antibody (9) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
  • also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
  • also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
  • Contact our Technical Service Department (or your local Distributor) for more information on how to receive a FREE 10 µg sample of α Enolase (9): sc-101513.
  • m-IgG1 BP-HRP is the preferred secondary detection reagent for α Enolase Antibody (9) for WB applications. This reagent is now offered in a bundle with α Enolase Antibody (9) (see ordering information below).

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α Enolase Antibody (9) is a mouse monoclonal IgG1 kappa light chain antibody that detects alpha Enolase protein of mouse, rat, and human origin by western blotting (WB), immunoprecipitation (IP), and enzyme-linked immunosorbent assay (ELISA). anti-alpha Enolase antibody (9) is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. alpha Enolase protein, also known as ENO1, plays a crucial role in the glycolytic pathway, facilitating the conversion of 2-phosphoglycerate to phosphoenolpyruvate, which is a key step in energy production. This enzymatic function is vital for cellular metabolism, particularly in tissues with high energy demands, such as muscle and brain. Notably, alpha Enolase is expressed in a wide range of tissues, while its isoforms, β enolase and γ enolase, are more restricted, with β primarily found in muscle and γ in nervous tissue. The presence of these isoforms as both homodimers and heterodimers underscores the complexity of metabolic regulation and the importance of alpha Enolase in maintaining cellular energy homeostasis.

For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.

Alexa Fluor® is a trademark of Molecular Probes Inc., OR., USA

LI-COR® and Odyssey® are registered trademarks of LI-COR Biosciences

Ordering Information

Product NameCatalog #UNITPriceQtyFAVORITES

α Enolase Antibody (9)

sc-101513
200 µg/ml
$316.00

α Enolase Antibody (9): m-IgG1 BP-HRP Bundle

sc-531653
200 µg Ab; 20 µg BP
$354.00

α Enolase Antibody (9) AC

sc-101513 AC
500 µg/ml, 25% agarose
$416.00

α Enolase Antibody (9) HRP

sc-101513 HRP
200 µg/ml
$316.00

α Enolase Antibody (9) FITC

sc-101513 FITC
200 µg/ml
$330.00

α Enolase Antibody (9) PE

sc-101513 PE
200 µg/ml
$343.00

α Enolase Antibody (9) Alexa Fluor® 488

sc-101513 AF488
200 µg/ml
$357.00

α Enolase Antibody (9) Alexa Fluor® 546

sc-101513 AF546
200 µg/ml
$357.00

α Enolase Antibody (9) Alexa Fluor® 594

sc-101513 AF594
200 µg/ml
$357.00

α Enolase Antibody (9) Alexa Fluor® 647

sc-101513 AF647
200 µg/ml
$357.00

α Enolase Antibody (9) Alexa Fluor® 680

sc-101513 AF680
200 µg/ml
$357.00

α Enolase Antibody (9) Alexa Fluor® 790

sc-101513 AF790
200 µg/ml
$357.00

Does this antibody is good for Flow cytometry? which cells can I use to show positive control

Asked by: sigalpo
Thank you for your question. The antibody conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 is recommended for Flow cytometry. Hela and A549 cells are good positive controls for the α Enolase protein.
Answered by: Tech Service
Date published: 2020-02-04

Are there any issues with using sc-101513: α Enolase (9) mouse monoclonal primary antibody on mouse samples?

Asked by: TinTin
Thank you for your inquiry. The use of mouse monoclonal antibodies with mouse samples is very common and typically poses no problem. To eliminate any potential cross-reactivity of an anti-mouse secondary detection reagent,we recommend using a directly conjugated primary antibody. Please contact our special orders department for pricing and availability of sc-101513 conjugates.
Answered by: Technical Support
Date published: 2017-03-07
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Rated 5 out of 5 by from Muy buen reconocimiento de la proteínaBuen reconocimiento de la enolasa en células C6/36 por western blot
Date published: 2025-01-29
Rated 5 out of 5 by from Western blotting of human tissues Alpha-enolasealpha Enolase Antibody sc-101513 works well for western blot analysis of human salivary gland tissues.
Date published: 2021-02-13
Rated 5 out of 5 by from Good specificity in WB in HeLa whole cell lysateGood specificity in WB in HeLa whole cell lysate. -SCBT QC
Date published: 2015-04-03
Rated 5 out of 5 by from Strong band in WB in human recombinant EnolaseStrong band in WB in human recombinant Enolase. -SCBT QC
Date published: 2014-06-27
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α Enolase Antibody (9) is rated 5.0 out of 5 by 4.
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