α-dystroglycan Antibody (D-3): sc-271589
- α-dystroglycan Antibody (D-3) is a mouse monoclonal IgG2a κ, cited in 1 publications, provided at 200 µg/ml
- raised against amino acids 30-329 mapping near the N-terminus of dystroglycan precursor of human origin
- Anti-alpha-dystroglycan Antibody (D-3) is recommended for detection of α-dystroglycan of mouse, rat and human origin by WB, IP, IF and ELISA
- Anti-alpha-dystroglycan Antibody (D-3) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
- also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- m-IgG Fc BP-HRP and m-IgG2a BP-HRP are the preferred secondary detection reagents for α-dystroglycan Antibody (D-3) for WB applications. These reagents are now offered in bundles with α-dystroglycan Antibody (D-3) (see ordering information below).
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α-dystroglycan Antibody (D-3) is a mouse monoclonal IgG2a kappa light chain antibody that detects α-dystroglycan in mouse, rat, and human samples through applications such as western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). α-dystroglycan (D-3) antibody is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. Dystroglycan functions as a cell surface receptor for several extracellular matrix components, including laminins, agrin, and perlecan, which are essential for basement membrane formation during early development and proper laminin organization on the cell surface. α-dystroglycan′s function is crucial in assembling the dystrophin-associated protein complex at the sarcolemma through dystrophin interaction, and the utrophin-associated protein complex at the neuromuscular junction through utrophin association. Additionally, α-dystroglycan facilitates nicotinic acetylcholine receptor clustering at the neuromuscular junction, highlighting a vital role in neuromuscular transmission and muscle function.
Alexa Fluor® is a trademark of Molecular Probes Inc., OR., USA
LI-COR® and Odyssey® are registered trademarks of LI-COR Biosciences
α-dystroglycan Antibody (D-3) References:
- Aberrant expression of alpha-dystroglycan in cervical and vulvar cancer. | Sgambato, A., et al. 2006. Gynecol Oncol. 103: 397-404. PMID: 16765426
- Loss of alpha-dystroglycan laminin binding in epithelium-derived cancers is caused by silencing of LARGE. | de Bernabé, DB., et al. 2009. J Biol Chem. 284: 11279-84. PMID: 19244252
- Reduced α-dystroglycan expression correlates with shortened patient survival in pancreatic cancer. | Jiang, X., et al. 2011. J Surg Res. 171: 120-6. PMID: 20338590
- Differential glycosylation of α-dystroglycan and proteins other than α-dystroglycan by like-glycosyltransferase. | Zhang, P. and Hu, H. 2012. Glycobiology. 22: 235-47. PMID: 21930648
- Fer kinase regulates cell migration through α-dystroglycan glycosylation. | Yoneyama, T., et al. 2012. Mol Biol Cell. 23: 771-80. PMID: 22238358
- The glucuronyltransferase B4GAT1 is required for initiation of LARGE-mediated α-dystroglycan functional glycosylation. | Willer, T., et al. 2014. Elife. 3: PMID: 25279699
- Multifaceted antibodies development against synthetic α-dystroglycan mucin glycopeptide as promising tools for dystroglycanopathies diagnostic. | Canassa-DeLeo, T., et al. 2020. Glycoconj J. 37: 77-93. PMID: 31823246
- Ribitol enhances matriglycan of α-dystroglycan in breast cancer cells without affecting cell growth. | Lu, PJ., et al. 2020. Sci Rep. 10: 4935. PMID: 32188898
- NAD+ enhances ribitol and ribose rescue of α-dystroglycan functional glycosylation in human FKRP-mutant myotubes. | Ortiz-Cordero, C., et al. 2021. Elife. 10: PMID: 33513091
- An atypical expression of core α-Dystroglycan and Laminin-α2 in skin fibroblasts of patients with congenital muscular dystrophies. | Sabry, S., et al. 2023. Mol Biol Rep. 50: 6373-6379. PMID: 37318662
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
α-dystroglycan Antibody (D-3) | sc-271589 | 200 µg/ml | $322.00 | |||
α-dystroglycan Antibody (D-3): m-IgG Fc BP-HRP Bundle | sc-525816 | 200 µg Ab; 10 µg BP | $361.00 | |||
α-dystroglycan Antibody (D-3): m-IgG2a BP-HRP Bundle | sc-546426 | 200 µg Ab; 10 µg BP | $361.00 | |||
α-dystroglycan Antibody (D-3) AC | sc-271589 AC | 500 µg/ml, 25% agarose | $424.00 | |||
α-dystroglycan Antibody (D-3) HRP | sc-271589 HRP | 200 µg/ml | $322.00 | |||
α-dystroglycan Antibody (D-3) FITC | sc-271589 FITC | 200 µg/ml | $336.00 | |||
α-dystroglycan Antibody (D-3) PE | sc-271589 PE | 200 µg/ml | $349.00 | |||
α-dystroglycan Antibody (D-3) Alexa Fluor® 488 | sc-271589 AF488 | 200 µg/ml | $364.00 | |||
α-dystroglycan Antibody (D-3) Alexa Fluor® 546 | sc-271589 AF546 | 200 µg/ml | $364.00 | |||
α-dystroglycan Antibody (D-3) Alexa Fluor® 594 | sc-271589 AF594 | 200 µg/ml | $364.00 | |||
α-dystroglycan Antibody (D-3) Alexa Fluor® 647 | sc-271589 AF647 | 200 µg/ml | $364.00 | |||
α-dystroglycan Antibody (D-3) Alexa Fluor® 680 | sc-271589 AF680 | 200 µg/ml | $364.00 | |||
α-dystroglycan Antibody (D-3) Alexa Fluor® 790 | sc-271589 AF790 | 200 µg/ml | $364.00 |