α-Adaptin 1/2 Antibody (AP.6): sc-32284
- α-Adaptin 1/2 Antibody (AP.6) is a mouse monoclonal IgG1 κ, cited in 4 publications, provided at 200 µg/ml
- raised against adaptor protein purified from bovine brain
- recommended for detection of α-Adaptin 1 and α-Adaptin 2, as well as an alternatively spliced isoform of α-Adaptin 1 found in neurons of mouse, rat and human origin by WB, IP, IF and IHC(P); also reactive with additional species, including bovine
- See α-Adaptin 1/2 (C-8): sc-17771 for α-Adaptin 1/2 antibody conjugates, including AC, HRP, FITC, PE, Alexa Fluor® 488, 594, 647, 680 and 790.
- m-IgG Fc BP-HRP and m-IgG1 BP-HRP are the preferred secondary detection reagents for α-Adaptin 1/2 Antibody (AP.6) for WB and IHC(P) applications. These reagents are now offered in bundles with α-Adaptin 1/2 Antibody (AP.6) (see ordering information below).
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α-Adaptin 1/2 Antibody (AP.6) is a mouse monoclonal IgG1 kappa light chain antibody that detects α-Adaptin 1 and α-Adaptin 2, as well as an alternatively spliced isoform of α-Adaptin 1 found in neurons of mouse, rat, and human origin through applications such as western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and immunohistochemistry. Anti-α-Adaptin 1/2 antibody (AP.6) plays a crucial role in the assembly of clathrin-coated pits and vesicles, which are essential for receptor-mediated endocytosis, a process vital for cellular communication and nutrient uptake. α-Adaptin 1/2 is primarily located in the cytoplasm and is involved in the transport of vesicles from the trans-Golgi network and the plasma membrane, facilitated by the adapter complexes AP-1 and AP-2. These complexes consist of clathrin-binding adaptin proteins, including γ-Adaptin and β-Adaptin for AP-1, and α-Adaptin 1, α-Adaptin 2, and β2-Adaptin for AP-2, along with smaller subunits known as AP50 and AP17. The structural organization of α-Adaptin, characterized by its two-domain architecture with variable C-terminal domains, is significant as this allows for the differential regulation of clathrin-mediated endocytosis in various tissues, highlighting the importance of splice variants in maintaining cellular function. Additionally, proteins such as AP180 and CALM, which are homologous to α-Adaptin, further underscore the complexity of clathrin assembly and its implications in synaptic function and cellular trafficking.
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α-Adaptin 1/2 Antibody (AP.6) References:
- A beta-turn endocytic code is required for optimal internalization of the growth hormone receptor but not for alpha-adaptin association. | Vleurick, L., et al. 1999. Mol Endocrinol. 13: 1823-31. PMID: 10551776
- The huntingtin interacting protein HIP1 is a clathrin and alpha-adaptin-binding protein involved in receptor-mediated endocytosis. | Waelter, S., et al. 2001. Hum Mol Genet. 10: 1807-17. PMID: 11532990
- Effect of clathrin heavy chain- and alpha-adaptin-specific small inhibitory RNAs on endocytic accessory proteins and receptor trafficking in HeLa cells. | Hinrichsen, L., et al. 2003. J Biol Chem. 278: 45160-70. PMID: 12960147
- 1H, 15N and 13C resonance assignments and 15N-1H residual dipolar couplings for the alpha-adaptin ear-domain. | Denisov, AY., et al. 2004. J Biomol NMR. 29: 441-2. PMID: 15213459
- A basic patch on alpha-adaptin is required for binding of human immunodeficiency virus type 1 Nef and cooperative assembly of a CD4-Nef-AP-2 complex. | Chaudhuri, R., et al. 2009. J Virol. 83: 2518-30. PMID: 19129443
- Interaction of Notch signaling modulator Numb with α-Adaptin regulates endocytosis of Notch pathway components and cell fate determination of neural stem cells. | Song, Y. and Lu, B. 2012. J Biol Chem. 287: 17716-17728. PMID: 22474327
- The appendage domain of alpha-adaptin is a high affinity binding site for dynamin. | Wang, LH., et al. 1995. J Biol Chem. 270: 10079-83. PMID: 7730311
- The sequence of a 22.4 kb DNA fragment from the left arm of yeast chromosome II reveals homologues to bacterial proline synthetase and murine alpha-adaptin, as well as a new permease and a DNA-binding protein. | De Wergifosse, P., et al. 1994. Yeast. 10: 1489-96. PMID: 7871888
- The conserved C-terminal domain of the bovine papillomavirus E5 oncoprotein can associate with an alpha-adaptin-like molecule: a possible link between growth factor receptors and viral transformation. | Cohen, BD., et al. 1993. Mol Cell Biol. 13: 6462-8. PMID: 8413245
- Adaptor self-aggregation, adaptor-receptor recognition and binding of alpha-adaptin subunits to the plasma membrane contribute to recruitment of adaptor (AP2) components of clathrin-coated pits. | Chang, MP., et al. 1993. EMBO J. 12: 2169-80. PMID: 8491205
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
α-Adaptin 1/2 Antibody (AP.6) | sc-32284 | 200 µg/ml | $316.00 | |||
α-Adaptin 1/2 Antibody (AP.6): m-IgG Fc BP-HRP Bundle | sc-538921 | 200 µg Ab; 10 µg BP | $354.00 | |||
α-Adaptin 1/2 Antibody (AP.6): m-IgG1 BP-HRP Bundle | sc-541151 | 200 µg Ab; 20 µg BP | $354.00 |