α-actinin Antibody (H-2): sc-17829

α-actinin Antibody (H-2) is a mouse monoclonal IgG1 kappa light chain antibody that detects α-actinin protein of mouse, rat, and human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), immunohistochemistry, and enzyme-linked immunosorbent assay (ELISA). α-actinin Antibody (H-2) is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. α-actinin plays a crucial role in the cytoskeleton by functioning as a bundling protein that cross-links filamentous actin (F-actin), thereby anchoring actin filaments to various intracellular structures. This structural integrity is vital for maintaining cell shape, enabling cellular movement, and facilitating muscle contraction. Notably, defects in the gene encoding α-actinin-4 can lead to focal segmental glomerulosclerosis 1 (FSGS1), a serious renal condition characterized by progressive kidney dysfunction and potential renal failure. The spectrin gene family, which includes α-actinins, is essential for the proper organization of the cytoskeleton, and understanding these proteins′ interactions and functions is critical for elucidating their roles in health and disease.

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α-actinin Antibody (H-2) References:

1. An alpha-actinin binding site of zyxin is essential for subcellular zyxin localization and alpha-actinin recruitment.  |  Reinhard, M., et al. 1999. J Biol Chem. 274: 13410-8. PMID: 10224105
2. Fine mapping of the alpha-actinin binding site within cysteine-rich protein.  |  Harper, BD., et al. 2000. Biochem J. 350 Pt 1: 269-74. PMID: 10926853
3. Interactions of a hemidesmosome component and actinin family members.  |  Gonzalez, AM., et al. 2001. J Cell Sci. 114: 4197-206. PMID: 11739652
4. Structural dynamics of alpha-actinin-vinculin interactions.  |  Bois, PR., et al. 2005. Mol Cell Biol. 25: 6112-22. PMID: 15988023
5. Alpha-actinin and vinculin in human neutrophils: reorganization during adhesion and relation to the actin network.  |  Yürüker, B. and Niggli, V. 1992. J Cell Sci. 101 (Pt 2): 403-14. PMID: 1629252
6. alpha-Actinin-dependent cytoskeletal anchorage is important for ICAM-5-mediated neuritic outgrowth.  |  Nyman-Huttunen, H., et al. 2006. J Cell Sci. 119: 3057-66. PMID: 16820411
7. Alpha-actinin structure and regulation.  |  Sjöblom, B., et al. 2008. Cell Mol Life Sci. 65: 2688-701. PMID: 18488141
8. Alpha-actinin: a multidisciplinary protein with important role in B-cell driven autoimmunity.  |  Oikonomou, KG., et al. 2011. Autoimmun Rev. 10: 389-96. PMID: 21241830
9. Expression of human alpha-actinin in human hepatocellular carcinoma.  |  Nishiyama, M., et al. 1990. Cancer Res. 50: 6291-4. PMID: 2169343
10. Cloning and chromosomal localization of the human cytoskeletal alpha-actinin gene reveals linkage to the beta-spectrin gene.  |  Youssoufian, H., et al. 1990. Am J Hum Genet. 47: 62-72. PMID: 2349951
11. Molecular basis of interactions between CaMKII and α-actinin-2 that underlie dendritic spine enlargement.  |  Curtis, AJ., et al. 2023. Elife. 12: PMID: 37489746
12. Interaction of alpha-actinin with the cadherin/catenin cell-cell adhesion complex via alpha-catenin.  |  Knudsen, KA., et al. 1995. J Cell Biol. 130: 67-77. PMID: 7790378

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-α-actinin Antibody (H-2) has 197 citations in a variety of scientific publications.

Primary antibodies like Anti-α-actinin Antibody (H-2) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

α-actinin Antibody (H-2) Specifications

Alternate Product Name: α-actinin

Product Clone Name: H-2

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG₁ kappa light chain

Amino Acids: 593-892

Epitope Target: α-actinin

Epitope Species: human

Product Reactivity/Detection: α-actinin isoforms

Species Reactivity/Detection: mouse, rat, human

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, IHC(P), ELISA

Vialing Information: Each vial contains 200 µg IgG₁ kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 197 citations in various scientific publications

Available Conjugates/Forms for α-actinin Antibody (H-2): AC: Agarose, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, FITC: Fluoroscein, HRP: Horseradish Peroxidase, PE: Phycoerythrin