α-actinin-4 Antibody (G-4): sc-390205

α-actinin-4 Antibody (G-4) is a mouse monoclonal IgG2a kappa light chain antibody that detects alpha-actinin-4 protein of mouse, rat, and human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), immunohistochemistry, and enzyme-linked immunosorbent assay (ELISA). anti-alpha-actinin-4 antibody (G-4) is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. Alpha-actinin-4 plays a crucial role in the cytoskeleton by cross-linking actin filaments, which is essential for maintaining cell shape, motility, and structural integrity. Alpha-actinin-4 is predominantly found in non-muscle cells, where alpha-actinin-4 contributes to cytoskeleton organization and is involved in various cellular processes, including cell adhesion and migration. Alpha-actinin-4′s presence in the cytoplasm and at the cell membrane underscores alpha-actinin-4′s importance in cellular dynamics and signaling pathways. Notably, mutations in the gene encoding alpha-actinin-4 have been linked to focal segmental glomerulosclerosis 1 (FSGS1), a serious kidney disorder characterized by progressive renal dysfunction, highlighting alpha-actinin-4′s significance in both health and disease.

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α-actinin-4 Antibody (G-4) References:

1. An alpha-actinin binding site of zyxin is essential for subcellular zyxin localization and alpha-actinin recruitment.  |  Reinhard, M., et al. 1999. J Biol Chem. 274: 13410-8. PMID: 10224105
2. Fine mapping of the alpha-actinin binding site within cysteine-rich protein.  |  Harper, BD., et al. 2000. Biochem J. 350 Pt 1: 269-74. PMID: 10926853
3. Interactions of a hemidesmosome component and actinin family members.  |  Gonzalez, AM., et al. 2001. J Cell Sci. 114: 4197-206. PMID: 11739652
4. Structural dynamics of alpha-actinin-vinculin interactions.  |  Bois, PR., et al. 2005. Mol Cell Biol. 25: 6112-22. PMID: 15988023
5. Alpha-actinin and vinculin in human neutrophils: reorganization during adhesion and relation to the actin network.  |  Yürüker, B. and Niggli, V. 1992. J Cell Sci. 101 (Pt 2): 403-14. PMID: 1629252
6. alpha-Actinin-dependent cytoskeletal anchorage is important for ICAM-5-mediated neuritic outgrowth.  |  Nyman-Huttunen, H., et al. 2006. J Cell Sci. 119: 3057-66. PMID: 16820411
7. Alpha-actinin-4 and CLP36 protein deficiencies contribute to podocyte defects in multiple human glomerulopathies.  |  Liu, Z., et al. 2011. J Biol Chem. 286: 30795-30805. PMID: 21680739
8. Expression of human alpha-actinin in human hepatocellular carcinoma.  |  Nishiyama, M., et al. 1990. Cancer Res. 50: 6291-4. PMID: 2169343
9. Podocyte Injury Associated with Mutant α-Actinin-4.  |  Cybulsky, AV. and Kennedy, CR. 2011. J Signal Transduct. 2011: 563128. PMID: 21808733
10. Cloning and chromosomal localization of the human cytoskeletal alpha-actinin gene reveals linkage to the beta-spectrin gene.  |  Youssoufian, H., et al. 1990. Am J Hum Genet. 47: 62-72. PMID: 2349951
11. Synaptopodin couples epithelial contractility to α-actinin-4-dependent junction maturation.  |  Kannan, N. and Tang, VW. 2015. J Cell Biol. 211: 407-34. PMID: 26504173
12. Interaction of alpha-actinin with the cadherin/catenin cell-cell adhesion complex via alpha-catenin.  |  Knudsen, KA., et al. 1995. J Cell Biol. 130: 67-77. PMID: 7790378

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-α-actinin-4 Antibody (G-4) has 16 citations in a variety of scientific publications.

Primary antibodies like Anti-α-actinin-4 Antibody (G-4) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

α-actinin-4 Antibody (G-4) Specifications

Alternate Product Name: α-actinin-4

Product Clone Name: G-4

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG_2a kappa light chain

Amino Acids: 610-660

Epitope Target: α-actinin-4

Epitope Species: human

Product Reactivity/Detection: α-actinin-4

Species Reactivity/Detection: mouse, rat, human

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, IHC(P), ELISA

Vialing Information: Each vial contains 200 µg IgG_2a kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 16 citations in various scientific publications

Available Conjugates/Forms for α-actinin-4 Antibody (G-4): AC: Agarose, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, FITC: Fluoroscein, HRP: Horseradish Peroxidase, PE: Phycoerythrin

The preferred α-actinin antibody is α-actinin Antibody (H-2): sc-17829.