alpha 3 Sodium Potassium ATPase/ATP1A3 CRISPR/Cas9 KO Plasmid (h): sc-416366

Overview

ATP1A3 encodes the alpha 3 catalytic subunit of the Na⁺/K⁺-transporting ATPase, a plasma membrane P-type ATPase that uses ATP hydrolysis to maintain transmembrane Na⁺ and K⁺ gradients. By shaping resting membrane potential, action potential recovery, and secondary transport, ATP1A3 influences neuronal excitability, ion homeostasis, and energy coupling across excitable tissues. Its activity is integrated with processes such as synaptic transmission, calcium handling, and cellular stress responses that depend on electrochemical gradients. Genetic and functional perturbations of ATP1A3 are linked to neurological disease phenotypes, making it a useful node for investigating mechanisms of neurophysiology and ion-transport dysfunction.

alpha 3 Sodium Potassium ATPase/ATP1A3 CRISPR/Cas9 KO Plasmid (h) is a pool of plasmids designed for targeted disruption of the ATP1A3 gene in human cell lines. Each plasmid co-expresses a unique single guide RNA (sgRNA) targeting a distinct site within the ATP1A3 together with the Streptococcus pyogenes Cas9 nuclease. The plasmids also encode GFP, allowing fluorescent identification and enrichment of successfully transfected cells by fluorescence microscopy or flow cytometry.

The multi-guide design increases the likelihood of generating insertions or deletions (indels) that disrupt the ATP1A3 open reading frame following Cas9-mediated double-strand break formation. DNA breaks introduced by the CRISPR/Cas9 system are repaired through endogenous non-homologous end joining (NHEJ) pathways, frequently resulting in frameshift mutations that abolish alpha 3 Sodium Potassium ATPase/ATP1A3 protein expression.

This CRISPR knockout system enables efficient generation of ATP1A3-deficient cell models for investigation of alpha 3 Sodium Potassium ATPase/ATP1A3 signaling, functional genomics studies, cancer biology research, and evaluation of therapeutic responses in human cell lines.

Key Features

• sgRNAs targeting ATP1A3 exon(s) critical for alpha 3 Sodium Potassium ATPase/ATP1A3 function
• Co-expression of SpCas9 and sgRNA from a single plasmid for simplified delivery
• GFP reporter for identification of transfected cells
• Pool of plasmids targeting multiple ATP1A3 genomic sites to improve knockout efficiency
• Compatible with delivery by transfection

Design Variants

CRISPRs +/- HDRs

• gRNAs encoded by alpha 3 Sodium Potassium ATPase/ATP1A3 CRISPR/Cas9 KO Plasmid (h) and alpha 3 Sodium Potassium ATPase/ATP1A3 CRISPR/Cas9 KO Plasmid (h2) target distinct sites within the ATP1A3 locus. One or both targeting designs may be available. See Related Products for availability.
• HDR donor constructs encoded by alpha 3 Sodium Potassium ATPase/ATP1A3 HDR Plasmid (h) and alpha 3 Sodium Potassium ATPase/ATP1A3 HDR Plasmid (h2) contain a puromycin resistance cassette and an RFP reporter flanked by ATP1A3 homology arms to support homology-directed repair at defined ATP1A3 target sites corresponding to the CRISPR/Cas9 KO designs. HDR donor availability may vary. See Related Products for availability.

For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.