alpha 3 Sodium Potassium ATPase/ATP1A3 Antibody (H-4): sc-365744

Na+/K+-ATPase α3 Antibody (H-4) is a mouse monoclonal IgG1 kappa light chain antibody that detects Na+/K+-ATPase α3 of mouse, rat, and human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). Na+/K+-ATPase α3 (H-4) antibody is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. Na+/K+-ATPase α3 is a vital component of the sodium/potassium-ATPase complex, which is crucial for maintaining cellular homeostasis. Na+/K+-ATPase α3 is primarily located in the plasma membrane of cells, where Na+/K+-ATPase α3 functions to transport sodium and potassium ions across the membrane, thereby regulating cellular volume, membrane potential, and nutrient uptake. Proper localization of Na+/K+-ATPase α3 is essential for ion transport, as any disruption can lead to cellular dysfunction and contribute to various pathophysiological conditions. The Na+/K+-ATPase complex is composed of multiple isoforms of three subunits: α, β, and γ, with the α subunit being responsible for ATP binding and cation transport. The β subunit plays a critical role in the correct folding and membrane insertion of the α subunit, while the γ subunit enhances the affinity of the complex for ATP in specific nephron segments. Understanding the localization and function of Na+/K+-ATPase α3 is important for elucidating Na+/K+-ATPase α3′s role in cellular physiology and potential implications in disease states.

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alpha 3 Sodium Potassium ATPase/ATP1A3 Antibody (H-4) References:

1. Functional role and immunocytochemical localization of the gamma a and gamma b forms of the Na,K-ATPase gamma subunit.  |  Pu, HX., et al. 2001. J Biol Chem. 276: 20370-8. PMID: 11278761
2. Chemical modification as an approach to elucidation of sodium pump structure-function relations.  |  Pedemonte, CH. and Kaplan, JH. 1990. Am J Physiol. 258: C1-23. PMID: 2154108
3. The sodium pump needs its beta subunit.  |  McDonough, AA., et al. 1990. FASEB J. 4: 1598-605. PMID: 2156741
4. Mutual dependence of Na,K-ATPase alpha- and beta-subunits for correct posttranslational processing and intracellular transport.  |  Ackermann, U. and Geering, K. 1990. FEBS Lett. 269: 105-8. PMID: 2167238
5. A proteolipid associated with Na,K-ATPase is not essential for ATPase activity.  |  Hardwicke, PM. and Freytag, JW. 1981. Biochem Biophys Res Commun. 102: 250-7. PMID: 6272791
6. Functional significance of the beta-subunit for heterodimeric P-type ATPases.  |  Chow, DC. and Forte, JG. 1995. J Exp Biol. 198: 1-17. PMID: 7891030
7. Na+,K(+)-ATPase.  |  Lingrel, JB. and Kuntzweiler, T. 1994. J Biol Chem. 269: 19659-62. PMID: 8051040
8. Subunit requirements for expression of functional sodium pumps in yeast cells.  |  Scheiner-Bobis, G. and Farley, RA. 1994. Biochim Biophys Acta. 1193: 226-34. PMID: 8054343
9. The alpha-subunit of the Na,K-ATPase has catalytic activity independent of the beta-subunit.  |  Blanco, G., et al. 1994. J Biol Chem. 269: 23420-5. PMID: 8089106
10. Expression, targeting, and assembly of functional Na,K-ATPase polypeptides in baculovirus-infected insect cells.  |  DeTomaso, AW., et al. 1993. J Biol Chem. 268: 1470-8. PMID: 8380413
11. Molecular cloning and immunological characterization of the gamma polypeptide, a small protein associated with the Na,K-ATPase.  |  Mercer, RW., et al. 1993. J Cell Biol. 121: 579-86. PMID: 8387529
12. Structure and function of the Na,K pump: ten years of molecular biology.  |  Pressley, TA. 1996. Miner Electrolyte Metab. 22: 264-71. PMID: 8933497

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-alpha 3 Sodium Potassium ATPase/ATP1A3 Antibody (H-4) has 5 citations in a variety of scientific publications.

Primary antibodies like Anti-alpha 3 Sodium Potassium ATPase/ATP1A3 Antibody (H-4) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

alpha 3 Sodium Potassium ATPase/ATP1A3 Antibody (H-4) Specifications

Alternate Product Name: ATP1A3

Product Clone Name: H-4

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG₁ kappa light chain

Amino Acids: 419-446

Epitope Target: Na+/K+- ATPase α3

Epitope Species: human

Product Reactivity/Detection: Na+/K+-ATPase α3

Species Reactivity/Detection: mouse, rat, human

Additional Mammalian Reactivity: equine, canine and porcine

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, ELISA

Vialing Information: Each vial contains 200 µg IgG₁ kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 5 citations in various scientific publications

Available Conjugates/Forms for alpha 3 Sodium Potassium ATPase/ATP1A3 Antibody (H-4): AC: Agarose, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, FITC: Fluoroscein, HRP: Horseradish Peroxidase, P: Blocking Peptide, PE: Phycoerythrin

The preferred ATP1A1/ATP1A2/ATP1A3 antibody is ATP1A1/ATP1A2/ATP1A3 Antibody (H-3): sc-48345.