ALKB Antibody (F-4): sc-374301

ALKB Antibody (F-4) is a mouse monoclonal IgG1 kappa light chain antibody that detects ALKB protein of human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). ALKB (F-4) antibody is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. ALKB protein plays a crucial role in cellular defense by repairing DNA and RNA damage caused by environmental methylating agents, specifically by reversing harmful alkylation lesions such as 1-methyladenine and 3-methylcytosine through oxidative demethylation. This process is vital for maintaining genomic integrity, as deficiencies in DNA and RNA repair mechanisms are linked to serious health issues, including cancer, neurological disorders, and developmental defects. Furthermore, ALKB is implicated in the cellular response to anti-cancer therapies, as ALKB helps tumors resist damage to their DNA, thereby contributing to treatment challenges. ALKB operates using ferrous iron and alpha-ketoglutarate as cofactors, with 2-oxoglutarate serving as a co-substrate and molecular oxygen as the oxidizing agent, highlighting complex biochemical interactions. Additionally, Escherichia coli ALKB protein is part of the superfamily of 2-oxoglutarate- and iron(II)-dependent oxygenases, underscoring evolutionary significance and functional diversity across different organisms.

Alexa Fluor^® is a trademark of Molecular Probes Inc., OR., USA

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ALKB Antibody (F-4) References:

1. Aberrant activity of the DNA repair enzyme AlkB.  |  Henshaw, TF., et al. 2004. J Inorg Biochem. 98: 856-61. PMID: 15134932
2. Demethylation of 3-methylthymine in DNA by bacterial and human DNA dioxygenases.  |  Koivisto, P., et al. 2004. J Biol Chem. 279: 40470-4. PMID: 15269201
3. Alkylation damage in DNA and RNA--repair mechanisms and medical significance.  |  Drabløs, F., et al. 2004. DNA Repair (Amst). 3: 1389-407. PMID: 15380096
4. AlkB restores the biological function of mRNA and tRNA inactivated by chemical methylation.  |  Ougland, R., et al. 2004. Mol Cell. 16: 107-16. PMID: 15469826
5. Repair of 3-methylthymine and 1-methylguanine lesions by bacterial and human AlkB proteins.  |  Falnes, PØ. 2004. Nucleic Acids Res. 32: 6260-7. PMID: 15576352
6. Direct removal of alkylation damage from DNA by AlkB and related DNA dioxygenases.  |  Sedgwick, B., et al. 2006. Methods Enzymol. 408: 108-20. PMID: 16793366

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-ALKB Antibody (F-4) has 4 citations in a variety of scientific publications.

Primary antibodies like Anti-ALKB Antibody (F-4) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

ALKB Antibody (F-4) Specifications

Alternate Product Name: ALKBH1

Product Clone Name: F-4

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG₁ kappa light chain

Amino Acids: 90-389

Epitope Target: ALKB

Epitope Species: human

Product Reactivity/Detection: ALKB

Species Reactivity/Detection: human

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, ELISA

Vialing Information: Each vial contains 200 µg IgG₁ kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 4 citations in various scientific publications

Available Conjugates/Forms for ALKB Antibody (F-4): AC: Agarose, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, FITC: Fluoroscein, HRP: Horseradish Peroxidase, PE: Phycoerythrin