Aldehyde dehydrogenase 8-A1/ALDH8A1 CRISPR/Cas9 KO Plasmid (h): sc-406326

Overview

Human ALDH8A1 encodes aldehyde dehydrogenase 8-A1, an NAD(P)+-dependent enzyme that oxidizes reactive aldehydes to their corresponding acids, contributing to cellular aldehyde detoxification and redox homeostasis. ALDH8A1 activity intersects with metabolic pathways handling lipid peroxidation–derived aldehydes and broader carbonyl stress responses, helping limit aldehyde-mediated protein and DNA adduct formation. Dysregulation of aldehyde dehydrogenase family enzymes is associated with altered oxidative stress tolerance, metabolic rewiring, and vulnerability to electrophile damage across multiple disease contexts. In biomedical research, ALDH8A1 is studied for its impact on mitochondrial and cytosolic metabolism, stress signaling, and cellular fitness under conditions that elevate endogenous aldehyde burden.

Aldehyde dehydrogenase 8-A1/ALDH8A1 CRISPR/Cas9 KO Plasmid (h) is a pool of plasmids designed for targeted disruption of the ALDH8A1 gene in human cell lines. Each plasmid co-expresses a unique single guide RNA (sgRNA) targeting a distinct site within the ALDH8A1 together with the Streptococcus pyogenes Cas9 nuclease. The plasmids also encode GFP, allowing fluorescent identification and enrichment of successfully transfected cells by fluorescence microscopy or flow cytometry.

The multi-guide design increases the likelihood of generating insertions or deletions (indels) that disrupt the ALDH8A1 open reading frame following Cas9-mediated double-strand break formation. DNA breaks introduced by the CRISPR/Cas9 system are repaired through endogenous non-homologous end joining (NHEJ) pathways, frequently resulting in frameshift mutations that abolish Aldehyde dehydrogenase 8-A1/ALDH8A1 protein expression.

This CRISPR knockout system enables efficient generation of ALDH8A1-deficient cell models for investigation of Aldehyde dehydrogenase 8-A1/ALDH8A1 signaling, functional genomics studies, cancer biology research, and evaluation of therapeutic responses in human cell lines.

Key Features

• sgRNAs targeting ALDH8A1 exon(s) critical for Aldehyde dehydrogenase 8-A1/ALDH8A1 function
• Co-expression of SpCas9 and sgRNA from a single plasmid for simplified delivery
• GFP reporter for identification of transfected cells
• Pool of plasmids targeting multiple ALDH8A1 genomic sites to improve knockout efficiency
• Compatible with delivery by transfection

Design Variants

CRISPRs +/- HDRs

• gRNAs encoded by Aldehyde dehydrogenase 8-A1/ALDH8A1 CRISPR/Cas9 KO Plasmid (h) and Aldehyde dehydrogenase 8-A1/ALDH8A1 CRISPR/Cas9 KO Plasmid (h2) target distinct sites within the ALDH8A1 locus. One or both targeting designs may be available. See Related Products for availability.
• HDR donor constructs encoded by Aldehyde dehydrogenase 8-A1/ALDH8A1 HDR Plasmid (h) and Aldehyde dehydrogenase 8-A1/ALDH8A1 HDR Plasmid (h2) contain a puromycin resistance cassette and an RFP reporter flanked by ALDH8A1 homology arms to support homology-directed repair at defined ALDH8A1 target sites corresponding to the CRISPR/Cas9 KO designs. HDR donor availability may vary. See Related Products for availability.

For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.