Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
Aldehyde dehydrogenase 4-A1/P5CDH/ALDH4A1 CRISPR/Cas9 KO Plasmid (m): sc-431814
- Target species: mouse
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- Aldehyde dehydrogenase 4-A1/P5CDH/ALDH4A1 CRISPR/Cas9 Knockout (KO) Plasmid (m) is a pool of plasmids, each encoding Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed for maximum knockout efficiency using sequences derived from the GeCKO v2 library
- gRNA sequences direct Cas9 to induce site-specific double-strand breaks (DSBs) in the Aldehyde dehydrogenase 4-A1/P5CDH/ALDH4A1 genomic locus, resulting in gene knockout through non-homologous end joining (NHEJ)
- The puromycin resistance and RFP genes are flanked by LoxP sites, enabling removal of selection markers via Cre recombinase (Cre Vector: sc-418923) after establishing stable knockout cell lines
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: Aldehyde dehydrogenase 4-A1/P5CDH/ALDH4A1 Antibody (F-1): sc-398911
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
Aldehyde dehydrogenase 4-A1/P5CDH/ALDH4A1 CRISPR/Cas9 KO Plasmid (m) | sc-431814 | 20 µg | $397.00 |
Overview
Mouse Aldh4a1 encodes aldehyde dehydrogenase 4-A1 (P5CDH/ALDH4A1), a mitochondrial enzyme that catalyzes conversion of Δ1-pyrroline-5-carboxylate to glutamate in the terminal steps of proline and hydroxyproline catabolism. By controlling the flux between proline metabolism, glutamate production, and mitochondrial redox balance, ALDH4A1 influences amino acid homeostasis and reactive aldehyde detoxification. Perturbation of this pathway is linked to altered oxidative stress handling and mitochondrial dysfunction, making Aldh4a1 a useful node for studying metabolic stress responses. ALDH4A1 activity also connects to broader nutrient-sensing and anaplerotic processes via glutamate-dependent entry into the TCA cycle.
Aldehyde dehydrogenase 4-A1/P5CDH/ALDH4A1 CRISPR/Cas9 KO Plasmid (m) is a pool of plasmids designed for targeted disruption of the Aldh4a1 gene in mouse cell lines. Each plasmid co-expresses a unique single guide RNA (sgRNA) targeting a distinct site within the Aldh4a1 together with the Streptococcus pyogenes Cas9 nuclease. The plasmids also encode GFP, allowing fluorescent identification and enrichment of successfully transfected cells by fluorescence microscopy or flow cytometry.
The multi-guide design increases the likelihood of generating insertions or deletions (indels) that disrupt the Aldh4a1 open reading frame following Cas9-mediated double-strand break formation. DNA breaks introduced by the CRISPR/Cas9 system are repaired through endogenous non-homologous end joining (NHEJ) pathways, frequently resulting in frameshift mutations that abolish Aldehyde dehydrogenase 4-A1/P5CDH/ALDH4A1 protein expression.
This CRISPR knockout system enables efficient generation of Aldh4a1-deficient cell models for investigation of Aldehyde dehydrogenase 4-A1/P5CDH/ALDH4A1 signaling, functional genomics studies, cancer biology research, and evaluation of therapeutic responses in human cell lines.
Key Features
- sgRNAs targeting Aldh4a1 exon(s) critical for Aldehyde dehydrogenase 4-A1/P5CDH/ALDH4A1 function
- Co-expression of SpCas9 and sgRNA from a single plasmid for simplified delivery
- GFP reporter for identification of transfected cells
- Pool of plasmids targeting multiple Aldh4a1 genomic sites to improve knockout efficiency
- Compatible with delivery by transfection
Design Variants
CRISPRs +/- HDRs
- gRNAs encoded by Aldehyde dehydrogenase 4-A1/P5CDH/ALDH4A1 CRISPR/Cas9 KO Plasmid (m) and Aldehyde dehydrogenase 4-A1/P5CDH/ALDH4A1 CRISPR/Cas9 KO Plasmid (m2) target distinct sites within the Aldh4a1 locus. One or both targeting designs may be available. See Related Products for availability.
- HDR donor constructs encoded by Aldehyde dehydrogenase 4-A1/P5CDH/ALDH4A1 HDR Plasmid (m) and Aldehyde dehydrogenase 4-A1/P5CDH/ALDH4A1 HDR Plasmid (m2) contain a puromycin resistance cassette and an RFP reporter flanked by Aldh4a1 homology arms to support homology-directed repair at defined Aldh4a1 target sites corresponding to the CRISPR/Cas9 KO designs. HDR donor availability may vary. See Related Products for availability.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.