ALAS-E Antibody (D-4): sc-166739

ALAS-E Antibody (D-4) is a mouse monoclonal IgG1 kappa light chain antibody that detects ALAS-E of mouse, rat, and human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), immunohistochemistry, and enzyme-linked immunosorbent assay (ELISA). ALAS-E (D-4) antibody is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. ALAS-E, also known as 5-aminolevulinate synthase 2, plays a crucial role in the heme biosynthetic pathway, specifically catalyzing the first step in heme synthesis, which is vital for oxygen transport and cellular respiration. This erythroid-specific isoenzyme is essential for hematopoietic cell differentiation and iron metabolism, particularly in the liver, where ALAS-E regulates heme availability for hemoglobin production. Notably, ALAS-E interacts with succinyl CoA, which induces a conformational change facilitating mitochondrial translocation, a critical step for function. ALAS-E regulation is influenced by transcription factors such as GATA-1, NF-E2, and EKLF, which bind to the 5′-flanking region, highlighting gene expression control importance in erythropoiesis. Since ALAS-E is located on the X chromosome, mutations can lead to pyridoxine-refractory X-linked sideroblastic anemia, underscoring clinical significance in hematological disorders.

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ALAS-E Antibody (D-4) References:

1. Interaction between succinyl CoA synthetase and the heme-biosynthetic enzyme ALAS-E is disrupted in sideroblastic anemia.  |  Furuyama, K. and Sassa, S. 2000. J Clin Invest. 105: 757-64. PMID: 10727444
2. Transcriptional regulation of the murine erythroid-specific 5-aminolevulinate synthase gene.  |  Kramer, MF., et al. 2000. Gene. 247: 153-66. PMID: 10773455
3. Transient state kinetic investigation of 5-aminolevulinate synthase reaction mechanism.  |  Zhang, J. and Ferreira, GC. 2002. J Biol Chem. 277: 44660-9. PMID: 12191993
4. The major splice variant of human 5-aminolevulinate synthase-2 contributes significantly to erythroid heme biosynthesis.  |  Cox, TC., et al. 2004. Int J Biochem Cell Biol. 36: 281-95. PMID: 14643893
5. Human erythroid 5-aminolevulinate synthase. Gene structure and species-specific differences in alternative RNA splicing.  |  Conboy, JG., et al. 1992. J Biol Chem. 267: 18753-8. PMID: 1527005
6. Fish oil feeding up-regulates the expression of 5-aminolevulinate synthase 2 mRNA in rat brain.  |  Haraguchi, T., et al. 2011. Biosci Biotechnol Biochem. 75: 1383-5. PMID: 21737922
7. Detection of Stimulated Erythropoiesis by the RNA-Based 5'-Aminolevulinate Synthase 2 Biomarker in Dried Blood Spot Samples.  |  Salamin, O., et al. 2019. Clin Chem. 65: 1563-1571. PMID: 31570629
8. The use of RNA-based 5'-aminolevulinate synthase 2 biomarkers in dried blood spots to detect recombinant human erythropoietin microdoses.  |  Loria, F., et al. 2022. Drug Test Anal. 14: 826-832. PMID: 34216436
9. Severe iron overload in a woman with homeostatic iron regulator (HFE) and a novel 5'-aminolevulinate synthase 2 (ALAS2) mutations: interactions of multiple genetic determinants.  |  de Gennes, C., et al. 2022. Br J Haematol. 196: e17-e20. PMID: 34490613
10. Mitochondrial Respiration in Peripheral Blood Mononuclear Cells Negatively Correlates with Disease Severity in Pulmonary Arterial Hypertension.  |  Sommer, N., et al. 2022. J Clin Med. 11: PMID: 35887896
11. mRNA biomarkers sensitive and specific to micro-dose erythropoietin treatment at sea level and altitude.  |  Loria, F., et al. 2024. Drug Test Anal. 16: 1392-1401. PMID: 38382494
12. ALAS2 overexpression alleviates oxidative stress-induced ferroptosis in aortic aneurysms via GATA1 activation.  |  He, Y., et al. 2024. J Thorac Dis. 16: 2510-2527. PMID: 38738239

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-ALAS-E Antibody (D-4) has 2 citations in a variety of scientific publications.

Primary antibodies like Anti-ALAS-E Antibody (D-4) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

ALAS-E Antibody (D-4) Specifications

Alternate Product Name: ALAS-E

Product Clone Name: D-4

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG₁ kappa light chain

Amino Acids: 61-80

Epitope Target: ALAS-E

Epitope Species: human

Product Reactivity/Detection: precursor and mature ALAS-E

Species Reactivity/Detection: mouse, rat, human

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, IHC(P), ELISA

Vialing Information: Each vial contains 200 µg IgG₁ kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 2 citations in various scientific publications

Available Conjugates/Forms for ALAS-E Antibody (D-4): AC: Agarose, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, FITC: Fluoroscein, HRP: Horseradish Peroxidase, P: Blocking Peptide, PE: Phycoerythrin