Akt1 Antibody (G-5): sc-55523

Akt1 Antibody (G-5) is a mouse monoclonal IgG1 kappa light chain antibody that detects Akt1 in mouse, rat, and human samples through applications such as western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). Anti-Akt1 antibody (G-5) is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. Akt1, also known as protein kinase B (PKB), plays a crucial role in cellular signaling pathways that regulate cell growth, survival, and metabolism. Activation of Akt1 begins when growth factors bind to their respective receptors, leading to phosphatidylinositol 3-kinase (PI3K) recruitment and subsequent Akt1 phosphorylation at Thr 308 and Ser 473. Thr 308 phosphorylation by phosphoinositide-dependent kinase-1 (PDK1) is critical for Akt1′s kinase activity, while Ser 473 phosphorylation, mediated by mTOR complex 2 (mTORC2), enhances enzymatic function and stabilizes active conformation. This dual phosphorylation enables Akt1 to propagate survival and growth signals and facilitates interaction with specific downstream substrates such as glycogen synthase kinase-3 beta (GSK-3β) and forkhead box proteins (FOXO), which play pivotal roles in metabolism and apoptosis. Precise spatial and temporal regulation of Akt1 activation is essential, as improper or prolonged activation can disrupt cellular homeostasis and lead to pathological conditions, including cancer and insulin resistance. Akt1 interactions with various downstream targets, including mammalian target of rapamycin (mTOR), are integral to protein synthesis and cell growth regulation, underscoring Akt1′s importance in cancer biology and metabolic disorders as a significant therapeutic target.

Alexa Fluor^® is a trademark of Molecular Probes Inc., OR., USA

LI-COR^® and Odyssey^® are registered trademarks of LI-COR Biosciences

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-Akt1 Antibody (G-5) has 50 citations in a variety of scientific publications.

Primary antibodies like Anti-Akt1 Antibody (G-5) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

Akt1 Antibody (G-5) Specifications

Alternate Product Name: Akt1

Product Clone Name: G-5

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG₁ kappa light chain

Amino Acids: 345-480

Epitope Target: Akt1

Epitope Species: human

Product Reactivity/Detection: Akt1

Species Reactivity/Detection: mouse, rat, human

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, ELISA

Vialing Information: Each vial contains 200 µg IgG₁ kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 50 citations in various scientific publications

Available Conjugates/Forms for Akt1 Antibody (G-5): AC: Agarose, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, FITC: Fluoroscein, HRP: Horseradish Peroxidase, PE: Phycoerythrin

The preferred Akt1 antibody is Akt1 Antibody (B-1): sc-5298.