AKR7A Antibody (F-8): sc-137186
- AKR7A Antibody (F-8) is a mouse monoclonal IgG2b κ AKR7A antibody, cited in 1 publications, provided at 200 µg/ml
- raised against amino acids 63-359 mapping at the C-terminus of AKR7A2 of human origin
- AKR7A Antibody (F-8) is recommended for detection of AKR7A2 and AKR7A3 of human origin, AKR7A5 of mouse origin and AKR7A2 of rat origin by WB, IP, IF and ELISA
- Anti-AKR7A Antibody (F-8) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
- also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- m-IgG2b BP-HRP and m-IgGκ BP-HRP are the preferred secondary detection reagents for AKR7A Antibody (F-8) for WB applications. These reagents are now offered in bundles with AKR7A Antibody (F-8) (see ordering information below).
AKR7A Antibody (F-8) is a mouse monoclonal IgG2b kappa light chain antibody that detects AKR7A protein of mouse, rat, and human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). Anti-AKR7A antibody (F-8) is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. The aldo-keto reductase 7 (AKR7) family, which includes AKR7A2, AKR7A3, and AKR7A4 in humans, as well as AKR7A5 in mice and AKR7A2 in rats, plays a crucial role in metabolizing Aflatoxin B1 and other dicarbonyl-containing compounds. AKR7A proteins are essential for metabolizing compounds with ketone groups on adjacent carbon atoms across various tissues, particularly in the liver, where these proteins help detoxify harmful substances. The human AKR7A2 gene is located on chromosome 1p36.13, a region often deleted in sporadic colorectal cancer, highlighting AKR7A2′s potential role as a tumor suppressor. AKR7A2′s functional importance lies in constitutive expression in the human liver, which aids in eliminating Aflatoxin, an environmental carcinogen, thereby serving as an endogenous chemopreventive agent. AKR7A3 functions as a homodimer and is expressed in various tissues, including the kidney, colon, pancreas, endometrium, and adenocarcinoma, further underscoring AKR7A proteins′ significance in both normal physiology and disease states.
Alexa Fluor® is a trademark of Molecular Probes Inc., OR., USA
LI-COR® and Odyssey® are registered trademarks of LI-COR Biosciences
AKR7A Antibody (F-8) References:
- Purification from rat liver of a novel constitutively expressed member of the aldo-keto reductase 7 family that is widely distributed in extrahepatic tissues. | Kelly, VP., et al. 2000. Biochem J. 348 Pt 2: 389-400. PMID: 10816434
- Novel homodimeric and heterodimeric rat gamma-hydroxybutyrate synthases that associate with the Golgi apparatus define a distinct subclass of aldo-keto reductase 7 family proteins. | Kelly, VP., et al. 2002. Biochem J. 366: 847-61. PMID: 12071861
- A novel aldo-keto reductase from Escherichia coli can increase resistance to methylglyoxal toxicity. | Grant, AW., et al. 2003. FEMS Microbiol Lett. 218: 93-9. PMID: 12583903
- The high resolution crystal structure of rat liver AKR7A1: understanding the substrate specificites of the AKR7 family. | Kozma, E., et al. 2003. Chem Biol Interact. 143-144: 289-97. PMID: 12604215
- The aldo-keto reductase superfamily homepage. | Hyndman, D., et al. 2003. Chem Biol Interact. 143-144: 621-31. PMID: 12604248
- Aflatoxin B1 aldehyde reductase (AFAR) genes cluster at 1p35-1p36.1 in a region frequently altered in human tumour cells. | Praml, C., et al. 2003. Oncogene. 22: 4765-73. PMID: 12879023
- Detoxication of the environmental pollutant acrolein by a rat liver aldo-keto reductase. | Gardner, R., et al. 2004. Toxicol Lett. 148: 65-72. PMID: 15019089
- Substrate specificity of an aflatoxin-metabolizing aldehyde reductase. | Ellis, EM. and Hayes, JD. 1995. Biochem J. 312 (Pt 2): 535-41. PMID: 8526867
- Molecular cloning, expression and catalytic activity of a human AKR7 member of the aldo-keto reductase superfamily: evidence that the major 2-carboxybenzaldehyde reductase from human liver is a homologue of rat aflatoxin B1-aldehyde reductase. | Ireland, LS., et al. 1998. Biochem J. 332 (Pt 1): 21-34. PMID: 9576847
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
AKR7A Antibody (F-8) | sc-137186 | 200 µg/ml | $316.00 | |||
AKR7A Antibody (F-8): m-IgGκ BP-HRP Bundle | sc-521446 | 200 µg Ab, 40 µg BP | $354.00 | |||
AKR7A Antibody (F-8): m-IgG2b BP-HRP Bundle | sc-548902 | 200 µg Ab; 10 µg BP | $354.00 | |||
AKR7A Antibody (F-8) AC | sc-137186 AC | 500 µg/ml, 25% agarose | $416.00 | |||
AKR7A Antibody (F-8) HRP | sc-137186 HRP | 200 µg/ml | $316.00 | |||
AKR7A Antibody (F-8) FITC | sc-137186 FITC | 200 µg/ml | $330.00 | |||
AKR7A Antibody (F-8) PE | sc-137186 PE | 200 µg/ml | $343.00 | |||
AKR7A Antibody (F-8) Alexa Fluor® 488 | sc-137186 AF488 | 200 µg/ml | $357.00 | |||
AKR7A Antibody (F-8) Alexa Fluor® 546 | sc-137186 AF546 | 200 µg/ml | $357.00 | |||
AKR7A Antibody (F-8) Alexa Fluor® 594 | sc-137186 AF594 | 200 µg/ml | $357.00 | |||
AKR7A Antibody (F-8) Alexa Fluor® 647 | sc-137186 AF647 | 200 µg/ml | $357.00 | |||
AKR7A Antibody (F-8) Alexa Fluor® 680 | sc-137186 AF680 | 200 µg/ml | $357.00 | |||
AKR7A Antibody (F-8) Alexa Fluor® 790 | sc-137186 AF790 | 200 µg/ml | $357.00 |