AKR1D1 Antibody (A-7): sc-373970
- AKR1D1 Antibody (A-7) is a mouse monoclonal IgG2a κ, cited in 1 publications, provided at 200 µg/ml
- specific for an epitope mapping between amino acids 13-37 at the N-terminus of AKR1D1 of human origin
- recommended for detection of AKR1D1 of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA; also reactive with additional species, including and equine, canine and bovine
- m-IgG Fc BP-HRP and m-IgGκ BP-HRP are the preferred secondary detection reagents for AKR1D1 Antibody (A-7) for WB and IHC(P) applications. These reagents are now offered in bundles with AKR1D1 Antibody (A-7) (see ordering information below).
AKR1D1 Antibody (A-7) is a mouse monoclonal IgG2a antibody that detects AKR1D1 in mouse, rat, and human samples through various applications including western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), immunohistochemistry with paraffin-embedded sections (IHCP), and enzyme-linked immunosorbent assay (ELISA). AKR1D1, also known as Δ(4)-3-oxosteroid 5-β-reductase or steroid 5-β-reductase, plays a crucial role in the metabolism of bile acid intermediates and steroid hormones by catalyzing the reduction of compounds with a Δ(4)-3-one structure to their 5-β reduced forms. This enzymatic activity is vital for maintaining hormonal balance and facilitating the detoxification of various substances, including drugs and carcinogens. AKR1D1 is predominantly expressed in the liver, colon, and testis, highlighting its importance in metabolic processes within these organs. Notably, AKR1D1 interacts with several key substrates, such as testosterone and progesterone, which are essential for reproductive health and metabolic regulation. A deficiency in AKR1D1 can lead to significant hepatic dysfunction, underscoring the importance of this protein in overall metabolic health. Anti-AKR1D1 antibody (A-7) is an invaluable tool for researchers studying steroid metabolism and related disorders.
Alexa Fluor® is a trademark of Molecular Probes Inc., OR., USA
LI-COR® and Odyssey® are registered trademarks of LI-COR Biosciences
AKR1D1 Antibody (A-7) References:
- Genetic variation in aldo-keto reductase 1D1 (AKR1D1) affects the expression and activity of multiple cytochrome P450s. | Chaudhry, AS., et al. 2013. Drug Metab Dispos. 41: 1538-47. PMID: 23704699
- 5β-Reduced steroids and human Δ(4)-3-ketosteroid 5β-reductase (AKR1D1). | Chen, M. and Penning, TM. 2014. Steroids. 83: 17-26. PMID: 24513054
- Cholic Acid to Treat HSD3B7 and AKR1D1 Deficiencies. | Jacquemin, E. and Gonzales, E. 2017. J Pediatr Gastroenterol Nutr. 65: e134. PMID: 28727657
- AKR1D1 regulates glucocorticoid availability and glucocorticoid receptor activation in human hepatoma cells. | Nikolaou, N., et al. 2019. J Steroid Biochem Mol Biol. 189: 218-227. PMID: 30769091
- AKR1D1*36 C>T (rs1872930) allelic variant is associated with variability of the CYP2C9 genotype predicted pharmacokinetics of ibuprofen enantiomers - a pilot study in healthy volunteers. | Kapedanovska Nestorovska, A., et al. 2019. Acta Pharm. 69: 399-412. PMID: 31259734
- AKR1D1 and CYP7B1 mutations in patients with inborn errors of bile acid metabolism: Possibly underdiagnosed diseases. | Chen, JY., et al. 2020. Pediatr Neonatol. 61: 75-83. PMID: 31337596
- The AKR1D1*36 (rs1872930) Allelic Variant Is Independently Associated With Clopidogrel Treatment Outcome. | Kapedanovska-Nestorovska, A., et al. 2019. Pharmgenomics Pers Med. 12: 287-295. PMID: 31695473
- The A-ring reduction of 11-ketotestosterone is efficiently catalysed by AKR1D1 and SRD5A2 but not SRD5A1. | Barnard, L., et al. 2020. J Steroid Biochem Mol Biol. 202: 105724. PMID: 32629108
- 5β-Dihydrosteroids: Formation and Properties. | Penning, TM. and Covey, DF. 2024. Int J Mol Sci. 25: PMID: 39201544
- Cloning, expression, and purification of recombinant AKR1D1 for therapeutic applications. | Shutevska, K. and Nestorovska, AK. 2024. Acta Pharm.. PMID: 39686626
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
AKR1D1 Antibody (A-7) | sc-373970 | 200 µg/ml | $322.00 | |||
AKR1D1 Antibody (A-7): m-IgG Fc BP-HRP Bundle | sc-537895 | 200 µg Ab; 10 µg BP | $361.00 | |||
AKR1D1 Antibody (A-7): m-IgGκ BP-HRP Bundle | sc-535353 | 200 µg Ab; 40 µg BP | $361.00 | |||
AKR1D1 (A-7) Neutralizing Peptide | sc-373970 P | 100 µg/0.5 ml | $69.00 |