AKAP 9 CRISPR/Cas9 KO Plasmid (h): sc-405430
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- AKAP 9 CRISPR/Cas9 Knockout (KO) Plasmid (h) is a pool of plasmids, each encoding Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed for maximum knockout efficiency using sequences derived from the GeCKO v2 library
- gRNA sequences direct Cas9 to induce site-specific double-strand breaks (DSBs) in the AKAP 9 genomic locus, resulting in gene knockout through non-homologous end joining (NHEJ)
- The puromycin resistance and RFP genes are flanked by LoxP sites, enabling removal of selection markers via Cre recombinase (Cre Vector: sc-418923) after establishing stable knockout cell lines
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: AKAP 9 Antibody (7E12): sc-517030
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
AKAP 9 CRISPR/Cas9 KO Plasmid (h) | sc-405430 | 20 µg | $397.00 |
Overview
AKAP9 encodes A-kinase anchoring protein 9 (AKAP 9), a large scaffold that spatially organizes PKA and additional signaling enzymes at defined subcellular sites. AKAP 9 localizes to centrosomes and the Golgi apparatus, supporting microtubule organization, Golgi integrity, and coordination of cell-cycle progression and mitotic spindle dynamics. Through compartmentalized kinase signaling, it influences phosphorylation-driven processes that regulate cytoskeletal remodeling and intracellular trafficking. Altered AKAP9 expression or rearrangements have been reported in cancer and other proliferative disorders, making it a useful target for studying dysregulated signaling networks and chromosomal stability.
AKAP 9 CRISPR/Cas9 KO Plasmid (h) is a pool of plasmids designed for targeted disruption of the AKAP9 gene in human cell lines. Each plasmid co-expresses a unique single guide RNA (sgRNA) targeting a distinct site within the AKAP9 together with the Streptococcus pyogenes Cas9 nuclease. The plasmids also encode GFP, allowing fluorescent identification and enrichment of successfully transfected cells by fluorescence microscopy or flow cytometry.
The multi-guide design increases the likelihood of generating insertions or deletions (indels) that disrupt the AKAP9 open reading frame following Cas9-mediated double-strand break formation. DNA breaks introduced by the CRISPR/Cas9 system are repaired through endogenous non-homologous end joining (NHEJ) pathways, frequently resulting in frameshift mutations that abolish AKAP 9 protein expression.
This CRISPR knockout system enables efficient generation of AKAP9-deficient cell models for investigation of AKAP 9 signaling, functional genomics studies, cancer biology research, and evaluation of therapeutic responses in human cell lines.
Key Features
- sgRNAs targeting AKAP9 exon(s) critical for AKAP 9 function
- Co-expression of SpCas9 and sgRNA from a single plasmid for simplified delivery
- GFP reporter for identification of transfected cells
- Pool of plasmids targeting multiple AKAP9 genomic sites to improve knockout efficiency
- Compatible with delivery by transfection
Design Variants
CRISPRs +/- HDRs
- gRNAs encoded by AKAP 9 CRISPR/Cas9 KO Plasmid (h) and AKAP 9 CRISPR/Cas9 KO Plasmid (h2) target distinct sites within the AKAP9 locus. One or both targeting designs may be available. See Related Products for availability.
- HDR donor constructs encoded by AKAP 9 HDR Plasmid (h) and AKAP 9 HDR Plasmid (h2) contain a puromycin resistance cassette and an RFP reporter flanked by AKAP9 homology arms to support homology-directed repair at defined AKAP9 target sites corresponding to the CRISPR/Cas9 KO designs. HDR donor availability may vary. See Related Products for availability.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.