AKAP 9 Antibody (7E12): sc-517030
- AKAP 9 Antibody (7E12) is a mouse monoclonal IgG2a κ AKAP 9 antibody provided at 100 µg/ml
- raised against amino acids 3812-3911 representing partial length AKAP 9 of human origin
- recommended for detection of AKAP 9 of human origin by WB, IP, IF and ELISA
- At present, we have not yet completed the identification of the preferred secondary detection reagent(s) for AKAP 9 Antibody (7E12). This work is in progress.
AKAP 9 Antibody (7E12) is a mouse monoclonal IgG2a kappa light chain antibody that detects AKAP 9 protein of human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). Anti-AKAP 9 antibody (7E12) is available as a non-conjugated format. AKAP 9 protein, also known as AKAP 450, is a large scaffolding protein consisting of 3,911 amino acids that plays a crucial role in the spatial organization of signaling pathways within cells. AKAP 9 is primarily localized to centrosomes and Golgi apparatus throughout cell cycles, which is essential for maintaining protein kinase A (PKA) signaling specificity. PKA compartmentalization is vital for function, allowing precise phosphorylation of substrates in distinct cellular locations, thereby influencing various cellular processes. AKAP 9 achieves this by anchoring PKA and other signaling molecules, facilitating interactions and ensuring signaling cascades activate only in appropriate cellular contexts. AKAP 9 undergoes alternative splicing, resulting in multiple isoforms, including AKAP 350 and Yotiao, with Yotiao predominantly expressed in pancreas and skeletal muscle. Yotiao isoform′s unique C-terminus contains an additional 12 amino acid sequence absent in AKAP 9, and interacts with NR1 subunit of NMDA receptor, promoting cAMP-dependent modulation of receptor activity at synapses. This interaction is significant for brain development and synaptic plasticity, highlighting AKAP 9′s importance in cellular signaling and neurological functions.
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AKAP 9 Antibody (7E12) References:
- Characterization of a novel giant scaffolding protein, CG-NAP, that anchors multiple signaling enzymes to centrosome and the golgi apparatus. | Takahashi, M., et al. 1999. J Biol Chem. 274: 17267-74. PMID: 10358086
- Yotiao protein, a ligand for the NMDA receptor, binds and targets cAMP-dependent protein kinase II(1). | Feliciello, A., et al. 1999. FEBS Lett. 464: 174-8. PMID: 10618500
- Requirement of a macromolecular signaling complex for beta adrenergic receptor modulation of the KCNQ1-KCNE1 potassium channel. | Marx, SO., et al. 2002. Science. 295: 496-9. PMID: 11799244
- A structural requirement for processing the cardiac K+ channel KCNQ1. | Kanki, H., et al. 2004. J Biol Chem. 279: 33976-83. PMID: 15140888
- Regulatory actions of the A-kinase anchoring protein Yotiao on a heart potassium channel downstream of PKA phosphorylation. | Kurokawa, J., et al. 2004. Proc Natl Acad Sci U S A. 101: 16374-8. PMID: 15528278
- Proarrhythmic consequences of a KCNQ1 AKAP-binding domain mutation: computational models of whole cells and heterogeneous tissue. | Saucerman, JJ., et al. 2004. Circ Res. 95: 1216-24. PMID: 15528464
- Autonomic control of cardiac action potentials: role of potassium channel kinetics in response to sympathetic stimulation. | Terrenoire, C., et al. 2005. Circ Res. 96: e25-34. PMID: 15731462
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
AKAP 9 Antibody (7E12) | sc-517030 | 100 µg/ml | $322.00 |