AKAP 82 Antibody (25): sc-135827

AKAP 82 Antibody (25) is a mouse monoclonal IgG1 kappa light chain antibody that detects AKAP 82 protein of mouse and rat origin by western blotting (WB) and immunoprecipitation (IP). Anti-AKAP 82 antibody (25) is available as the non-conjugated format. The AKAP (A-kinase anchoring proteins) family plays a crucial role in cellular signaling by anchoring protein kinase A (PKA) to specific cellular locations, thereby ensuring precise regulation of PKA activity. AKAP 82, also known as PRKA4 (protein kinase A-anchoring protein 4) or major sperm fibrous sheath protein, is predominantly expressed in spermatids during spermiogenesis, where AKAP 82 is essential for fibrous sheath assembly, a structure critical for sperm motility and function. AKAP 82 features two distinct binding sites for PKA, one specific for the regulatory subunit RIα and another that can accommodate either RIα or RIIα, which underscores versatility in signaling pathways. AKAP 82 interacts with AKAP 3, forming a significant component of the fibrous sheath structure. Absence of AKAP 82 in knockout models leads to a marked reduction or loss of associated proteins such as AKAP 3, RIIα, SP17, and GAPDS, which can result in compromised sperm function and may contribute to conditions like asthenospermia. AKAP 82′s ability to facilitate precise PKA localization is vital for maintaining signaling pathway fidelity that governs spermatogenesis and sperm motility.

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AKAP 82 Antibody (25) References:

1. Conservation and function of a bovine sperm A-kinase anchor protein homologous to mouse AKAP82.  |  Moss, SB., et al. 1999. Biol Reprod. 61: 335-42. PMID: 10411509
2. Relationship between sperm motility and the processing and tyrosine phosphorylation of two human sperm fibrous sheath proteins, pro-hAKAP82 and hAKAP82.  |  Turner, RM., et al. 1999. Mol Hum Reprod. 5: 816-24. PMID: 10460219
3. A-kinase anchoring protein 4 binding proteins in the fibrous sheath of the sperm flagellum.  |  Brown, PR., et al. 2003. Biol Reprod. 68: 2241-8. PMID: 12606363
4. Association of sperm protein 17 with A-kinase anchoring protein 3 in flagella.  |  Lea, IA., et al. 2004. Reprod Biol Endocrinol. 2: 57. PMID: 15257753
5. Changes in intracellular distribution and activity of protein phosphatase PP1gamma2 and its regulating proteins in spermatozoa lacking AKAP4.  |  Huang, Z., et al. 2005. Biol Reprod. 72: 384-92. PMID: 15385410
6. Differential RNA expression and polyribosome loading of alternative transcripts of the Akap4 gene in murine spermatids.  |  Nipper, RW., et al. 2005. Mol Reprod Dev. 70: 397-405. PMID: 15685631
7. [AKAP82 expression in sperm in asthenospermia].  |  Li, Y., et al. 2005. Zhonghua Nan Ke Xue. 11: 908-11. PMID: 16398361
8. Delineation of type I protein kinase A-selective signaling events using an RI anchoring disruptor.  |  Carlson, CR., et al. 2006. J Biol Chem. 281: 21535-21545. PMID: 16728392
9. Transmission electron microscopy, immunocytochemical and fluorescence in situ hybridisation studies in a case of 100% necrozoospermia: case report.  |  Moretti, E., et al. 2006. Andrologia. 38: 233-8. PMID: 17081177

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-AKAP 82 Antibody (25) has 2 citations in a variety of scientific publications.

Primary antibodies like Anti-AKAP 82 Antibody (25) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

AKAP 82 Antibody (25) Specifications

Alternate Product Name: AKAP 82

Product Clone Name: 25

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG₁ kappa light chain

Amino Acids: 555-675

Epitope Target: AKAP 82

Epitope Species: mouse

Product Reactivity/Detection: AKAP 82

Species Reactivity/Detection: mouse, rat

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP

Vialing Information: Each vial contains 200 µg IgG₁ kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 2 citations in various scientific publications

Available Conjugates/Forms for AKAP 82 Antibody (25): N/A