Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
AKAP 79 CRISPR/Cas9 KO Plasmid (h): sc-402647
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- AKAP 79 CRISPR/Cas9 Knockout (KO) Plasmid (h) is a pool of plasmids, each encoding Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed for maximum knockout efficiency using sequences derived from the GeCKO v2 library
- gRNA sequences direct Cas9 to induce site-specific double-strand breaks (DSBs) in the AKAP 79 genomic locus, resulting in gene knockout through non-homologous end joining (NHEJ)
- The puromycin resistance and RFP genes are flanked by LoxP sites, enabling removal of selection markers via Cre recombinase (Cre Vector: sc-418923) after establishing stable knockout cell lines
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: AKAP 79 Antibody (D-9): sc-17772
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
AKAP 79 CRISPR/Cas9 KO Plasmid (h) | sc-402647 | 20 µg | $397.00 |
Overview
AKAP5 encodes AKAP 79, a membrane-associated A-kinase anchoring protein that organizes multiprotein signaling complexes by tethering PKA alongside phosphatases such as calcineurin and PP2B to defined subcellular microdomains. By scaffolding kinases, phosphatases, and receptors, AKAP 79 shapes signal amplitude and duration in pathways controlling synaptic plasticity, receptor trafficking, and activity-dependent phosphorylation, including calcium- and cAMP-regulated processes. In neurons and excitable tissues, this spatial regulation influences ion channel modulation and downstream transcriptional responses. Dysregulated AKAP 79 signaling has been implicated in altered neuronal excitability and network function, making AKAP5 a useful node for studying mechanisms relevant to neurological disease biology and stress-responsive signaling.
AKAP 79 CRISPR/Cas9 KO Plasmid (h) is a pool of plasmids designed for targeted disruption of the AKAP5 gene in human cell lines. Each plasmid co-expresses a unique single guide RNA (sgRNA) targeting a distinct site within the AKAP5 together with the Streptococcus pyogenes Cas9 nuclease. The plasmids also encode GFP, allowing fluorescent identification and enrichment of successfully transfected cells by fluorescence microscopy or flow cytometry.
The multi-guide design increases the likelihood of generating insertions or deletions (indels) that disrupt the AKAP5 open reading frame following Cas9-mediated double-strand break formation. DNA breaks introduced by the CRISPR/Cas9 system are repaired through endogenous non-homologous end joining (NHEJ) pathways, frequently resulting in frameshift mutations that abolish AKAP 79 protein expression.
This CRISPR knockout system enables efficient generation of AKAP5-deficient cell models for investigation of AKAP 79 signaling, functional genomics studies, cancer biology research, and evaluation of therapeutic responses in human cell lines.
Key Features
- sgRNAs targeting AKAP5 exon(s) critical for AKAP 79 function
- Co-expression of SpCas9 and sgRNA from a single plasmid for simplified delivery
- GFP reporter for identification of transfected cells
- Pool of plasmids targeting multiple AKAP5 genomic sites to improve knockout efficiency
- Compatible with delivery by transfection
Design Variants
CRISPRs +/- HDRs
- gRNAs encoded by AKAP 79 CRISPR/Cas9 KO Plasmid (h) and AKAP 79 CRISPR/Cas9 KO Plasmid (h2) target distinct sites within the AKAP5 locus. One or both targeting designs may be available. See Related Products for availability.
- HDR donor constructs encoded by AKAP 79 HDR Plasmid (h) and AKAP 79 HDR Plasmid (h2) contain a puromycin resistance cassette and an RFP reporter flanked by AKAP5 homology arms to support homology-directed repair at defined AKAP5 target sites corresponding to the CRISPR/Cas9 KO designs. HDR donor availability may vary. See Related Products for availability.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.