AKAP 150 Antibody (E-1): sc-377055

AKAP 150 Antibody (E-1) is a mouse monoclonal IgG1 kappa light chain antibody that detects AKAP 150 protein of mouse and rat origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). Anti-AKAP 150 antibody (E-1) is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. AKAP 150 plays a crucial role in the spatial organization of signaling pathways within cells, particularly in cAMP-dependent protein kinase (PKA) signaling. AKAP 150 is primarily localized in the postsynaptic densities of neurons, where AKAP 150 anchors PKA to specific sites, facilitating precise signaling events essential for synaptic plasticity and memory formation. AKAP 150′s ability to compartmentalize PKA is vital for maintaining PKA signaling specificity, ensuring activation only in appropriate cellular contexts. Additionally, AKAP 150 interacts with other proteins, such as MAP2 and AKAP 79, which are also involved in PKA anchoring, highlighting these interactions′ importance in neuronal signaling pathway regulation. Using AKAP 150 monoclonal antibody (E-1) enables researchers to gain valuable insights into synaptic function mechanisms and PKA′s role in neuronal signaling.

Alexa Fluor^® is a trademark of Molecular Probes Inc., OR., USA

LI-COR^® and Odyssey^® are registered trademarks of LI-COR Biosciences

AKAP 150 Antibody (E-1) References:

1. LTP but not seizure is associated with up-regulation of AKAP-150.  |  Génin, A., et al. 2003. Eur J Neurosci. 17: 331-40. PMID: 12542670
2. Regulation of Na,K-ATPase by cAMP-dependent protein kinase anchored on membrane via A-kinase anchoring protein subtype, AKAP-150, in rat parotid gland.  |  Kurihara, K. and Nakanishi, N. 2003. Ann N Y Acad Sci. 986: 636-8. PMID: 12763907
3. Specific expression of an A-kinase anchoring protein subtype, AKAP-150, and specific regulatory mechanism for Na(+),K(+)-ATPase via protein kinase A in the parotid gland among the three major salivary glands of the rat.  |  Kurihara, K., et al. 2003. Biochem Pharmacol. 66: 239-50. PMID: 12826266
4. cAMP signaling in neurons: patterns of neuronal expression and intracellular localization for a novel protein, AKAP 150, that anchors the regulatory subunit of cAMP-dependent protein kinase II beta.  |  Glantz, SB., et al. 1992. Mol Biol Cell. 3: 1215-28. PMID: 1333841
5. Localization of the cAMP-dependent protein kinase to the postsynaptic densities by A-kinase anchoring proteins. Characterization of AKAP 79.  |  Carr, DW., et al. 1992. J Biol Chem. 267: 16816-23. PMID: 1512224
6. Type II regulatory subunit dimerization determines the subcellular localization of the cAMP-dependent protein kinase.  |  Scott, JD., et al. 1990. J Biol Chem. 265: 21561-6. PMID: 2147685
7. AKAP150 Palmitoylation Regulates Synaptic Incorporation of Ca2+-Permeable AMPA Receptors to Control LTP.  |  Purkey, AM., et al. 2018. Cell Rep. 25: 974-987.e4. PMID: 30355502
8. AKAP150 from nucleus accumbens dopamine D1 and D2 receptor-expressing medium spiny neurons regulates morphine withdrawal.  |  Bai, X., et al. 2023. iScience. 26: 108227. PMID: 37953959
9. Association of protein kinase A and protein phosphatase 2B with a common anchoring protein.  |  Coghlan, VM., et al. 1995. Science. 267: 108-11. PMID: 7528941
10. A-kinase anchoring proteins: a key to selective activation of cAMP-responsive events?  |  Coghlan, VM., et al. 1993. Mol Cell Biochem. 127-128: 309-19. PMID: 7935359
11. Cloning and characterization of AKAP 95, a nuclear protein that associates with the regulatory subunit of type II cAMP-dependent protein kinase.  |  Coghlan, VM., et al. 1994. J Biol Chem. 269: 7658-65. PMID: 8125992
12. Cloning and characterization of a novel A-kinase anchoring protein. AKAP 220, association with testicular peroxisomes.  |  Lester, LB., et al. 1996. J Biol Chem. 271: 9460-5. PMID: 8621616

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-AKAP 150 Antibody (E-1) has 9 citations in a variety of scientific publications.

Primary antibodies like Anti-AKAP 150 Antibody (E-1) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

AKAP 150 Antibody (E-1) Specifications

Alternate Product Name: AKAP 150

Product Clone Name: E-1

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG₁ kappa light chain

Amino Acids: 267-478

Epitope Target: AKAP 150

Epitope Species: rat

Product Reactivity/Detection: AKAP 150

Species Reactivity/Detection: mouse, rat

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, ELISA

Vialing Information: Each vial contains 200 µg IgG₁ kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 9 citations in various scientific publications

Available Conjugates/Forms for AKAP 150 Antibody (E-1): AC: Agarose, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, FITC: Fluoroscein, HRP: Horseradish Peroxidase, PE: Phycoerythrin