AKAP 149 Antibody (B-10): sc-377450

AKAP 149 Antibody (B-10) is a mouse monoclonal IgG2b kappa light chain antibody that detects AKAP 149 protein of mouse, rat, and human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). Anti-AKAP 149 antibody (B-10) is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. AKAP 149 plays a crucial role in the spatial and temporal regulation of signaling pathways by anchoring protein kinase A (PKA) to specific cellular locations, which is essential for maintaining PKA signaling specificity. This compartmentalization allows for precise control over various cellular processes, including metabolism, gene expression, and cell growth. AKAP 149, a splice variant of S-AKAP 84, is particularly important in the phosphorylation-dependent regulation of RNA processing, highlighting its role in gene expression regulation. Additionally, AKAP 149 interacts with other proteins, such as MAP2 and neuronally expressed AKAP 79 and AKAP 150, which further emphasizes its significance in diverse cellular functions and tissue specificity.

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AKAP 149 Antibody (B-10) References:

1. AMY-1, a c-Myc-binding protein, is localized in the mitochondria of sperm by association with S-AKAP84, an anchor protein of cAMP-dependent protein kinase.  |  Furusawa, M., et al. 2001. J Biol Chem. 276: 36647-51. PMID: 11483602
2. AMY-1 interacts with S-AKAP84 and AKAP95 in the cytoplasm and the nucleus, respectively, and inhibits cAMP-dependent protein kinase activity by preventing binding of its catalytic subunit to A-kinase-anchoring protein (AKAP) complex.  |  Furusawa, M., et al. 2002. J Biol Chem. 277: 50885-92. PMID: 12414807
3. Localization of the cAMP-dependent protein kinase to the postsynaptic densities by A-kinase anchoring proteins. Characterization of AKAP 79.  |  Carr, DW., et al. 1992. J Biol Chem. 267: 16816-23. PMID: 1512224
4. Type II regulatory subunit dimerization determines the subcellular localization of the cAMP-dependent protein kinase.  |  Scott, JD., et al. 1990. J Biol Chem. 265: 21561-6. PMID: 2147685
5. Role of AKAP 149-PKA-PDE4A complex in cell survival and cell differentiation processes.  |  Fernández-Araujo, A., et al. 2014. Int J Biochem Cell Biol. 53: 89-101. PMID: 24813785
6. A-Kinase Anchor Protein 1 deficiency causes mitochondrial dysfunction in mouse model of hyperoxia induced acute lung injury.  |  Soundararajan, R., et al. 2022. Front Pharmacol. 13: 980723. PMID: 36263130
7. Characterization of S-AKAP84, a novel developmentally regulated A kinase anchor protein of male germ cells.  |  Lin, RY., et al. 1995. J Biol Chem. 270: 27804-11. PMID: 7499250
8. Association of protein kinase A and protein phosphatase 2B with a common anchoring protein.  |  Coghlan, VM., et al. 1995. Science. 267: 108-11. PMID: 7528941
9. A-kinase anchoring proteins: a key to selective activation of cAMP-responsive events?  |  Coghlan, VM., et al. 1993. Mol Cell Biochem. 127-128: 309-19. PMID: 7935359
10. Cloning and characterization of AKAP 95, a nuclear protein that associates with the regulatory subunit of type II cAMP-dependent protein kinase.  |  Coghlan, VM., et al. 1994. J Biol Chem. 269: 7658-65. PMID: 8125992
11. Cloning and characterization of a novel A-kinase anchoring protein. AKAP 220, association with testicular peroxisomes.  |  Lester, LB., et al. 1996. J Biol Chem. 271: 9460-5. PMID: 8621616
12. Molecular characterization of AKAP149, a novel A kinase anchor protein with a KH domain.  |  Trendelenburg, G., et al. 1996. Biochem Biophys Res Commun. 225: 313-9. PMID: 8769136

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-AKAP 149 Antibody (B-10) has 1 citations in a variety of scientific publications.

Primary antibodies like Anti-AKAP 149 Antibody (B-10) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

AKAP 149 Antibody (B-10) Specifications

Alternate Product Name: AKAP1

Product Clone Name: B-10

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG_2b kappa light chain

Amino Acids: 604-903

Epitope Target: AKAP 149

Epitope Species: human

Product Reactivity/Detection: AKAP 149

Species Reactivity/Detection: mouse, rat, human

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, ELISA

Vialing Information: Each vial contains 200 µg IgG_2b kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 1 citations in various scientific publications

Available Conjugates/Forms for AKAP 149 Antibody (B-10): AC: Agarose, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, FITC: Fluoroscein, HRP: Horseradish Peroxidase, PE: Phycoerythrin