AK3 Antibody (E-5): sc-398571
- AK3 Antibody (E-5) is a mouse monoclonal IgG1 κ AK3 antibody, cited in 2 publications, provided at 200 µg/ml
- specific for an epitope mapping between amino acids 209-227 at the C-terminus of AK3 of human origin
- AK3 Antibody (E-5) is recommended for detection of AK3 of mouse, rat and human origin by WB, IP, IF and ELISA
- Anti-AK3 Antibody (E-5) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
- also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- m-IgG Fc BP-HRP, m-IgG1 BP-HRP and m-IgGκ BP-HRP are the preferred secondary detection reagents for AK3 Antibody (E-5) for WB applications. These reagents are now offered in bundles with AK3 Antibody (E-5) (see ordering information below).
AK3 Antibody (E-5) is a mouse monoclonal IgG1 kappa light chain antibody that detects AK3 protein of mouse, rat, and human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). AK3 (E-5) antibody is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. AK3 plays a crucial role in cellular energy metabolism as AK3 is involved in the phosphorylation of adenine nucleotides, specifically utilizing guanosine triphosphate (GTP) as a substrate, which distinguishes AK3 from other isoforms like AK1 and AK2 that primarily use adenosine triphosphate (ATP). This unique substrate preference is significant because AK3 participates in specific metabolic pathways vital for maintaining energy homeostasis in cells, particularly in the mitochondria of liver and heart tissues. Mutations in AK3 can lead to metabolic dysfunctions, highlighting AK3′s importance in cellular health and disease. With AK3′s ability to influence metabolic signals that affect gene expression and protein kinase-mediated signaling, anti-AK3 antibody (E-5) serves as a valuable tool for researchers studying energy metabolism and related disorders.
Alexa Fluor® is a trademark of Molecular Probes Inc., OR., USA
LI-COR® and Odyssey® are registered trademarks of LI-COR Biosciences
AK3 Antibody (E-5) References:
- Cloning and functional characterization of the promoter region of the gene encoding human adenylate kinase isozyme 3. | Noma, T., et al. 1999. Biochem Biophys Res Commun. 264: 990-7. PMID: 10544043
- Characterization of the 5'-flanking region of the gene encoding bovine adenylate kinase isozyme 3. | Noma, T., et al. 1999. Biochim Biophys Acta. 1489: 383-8. PMID: 10673039
- Adenylate kinase phosphotransfer communicates cellular energetic signals to ATP-sensitive potassium channels. | Carrasco, AJ., et al. 2001. Proc Natl Acad Sci U S A. 98: 7623-8. PMID: 11390963
- Characterization of human adenylate kinase 3 (AK3) cDNA and mapping of the AK3 pseudogene to an intron of the NF1 gene. | Xu, G., et al. 1992. Genomics. 13: 537-42. PMID: 1639383
- Cloning and characterization of the gene encoding bovine mitochondrial adenylate kinase isozyme 3. | Shahjahan, M., et al. 1991. Gene. 107: 313-7. PMID: 1748300
- Mechanisms of toxicity of 3'-azido-3'-deoxythymidine. Its interaction with adenylate kinase. | Barile, M., et al. 1994. Biochem Pharmacol. 48: 1405-12. PMID: 7945440
- Adenylate kinase: kinetic behavior in intact cells indicates it is integral to multiple cellular processes. | Dzeja, PP., et al. 1998. Mol Cell Biochem. 184: 169-82. PMID: 9746320
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
AK3 Antibody (E-5) | sc-398571 | 200 µg/ml | $316.00 | |||
AK3 Antibody (E-5): m-IgG Fc BP-HRP Bundle | sc-530846 | 200 µg Ab; 10 µg BP | $354.00 | |||
AK3 Antibody (E-5): m-IgGκ BP-HRP Bundle | sc-524394 | 200 µg Ab, 40 µg BP | $354.00 | |||
AK3 Antibody (E-5): m-IgG1 BP-HRP Bundle | sc-544276 | 200 µg Ab; 20 µg BP | $354.00 | |||
AK3 Antibody (E-5) AC | sc-398571 AC | 500 µg/ml, 25% agarose | $416.00 | |||
AK3 Antibody (E-5) HRP | sc-398571 HRP | 200 µg/ml | $316.00 | |||
AK3 Antibody (E-5) FITC | sc-398571 FITC | 200 µg/ml | $330.00 | |||
AK3 Antibody (E-5) PE | sc-398571 PE | 200 µg/ml | $343.00 | |||
AK3 Antibody (E-5) Alexa Fluor® 488 | sc-398571 AF488 | 200 µg/ml | $357.00 | |||
AK3 Antibody (E-5) Alexa Fluor® 546 | sc-398571 AF546 | 200 µg/ml | $357.00 | |||
AK3 Antibody (E-5) Alexa Fluor® 594 | sc-398571 AF594 | 200 µg/ml | $357.00 | |||
AK3 Antibody (E-5) Alexa Fluor® 647 | sc-398571 AF647 | 200 µg/ml | $357.00 | |||
AK3 Antibody (E-5) Alexa Fluor® 680 | sc-398571 AF680 | 200 µg/ml | $357.00 | |||
AK3 Antibody (E-5) Alexa Fluor® 790 | sc-398571 AF790 | 200 µg/ml | $357.00 | |||
AK3 (E-5) Neutralizing Peptide | sc-398571 P | 100 µg/0.5 ml | $68.00 |