AK1 Antibody (G-9): sc-365048

AK1 Antibody (G-9) is a mouse monoclonal IgG2a light chain antibody that detects adenylate kinase 1 (AK1) in mouse, rat, and human samples through applications such as western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). AK1 plays a crucial role in cellular energy metabolism by catalyzing the transfer of phosphate groups between adenine nucleotides, which are vital for energy transfer and storage in cells. Anti-AK1 antibody (G-9) recognizes the enzyme predominantly in the cytosol of skeletal muscle, brain, and erythrocytes, where AK1 is often found clustered within myofibrils or associated with cellular membranes. Proper localization of AK1 is essential for function, ensuring efficient energy transfer during muscle contraction and metabolic processes. Deficiencies in AK1 due to inherited mutations can lead to significant health issues, such as hemolytic anemia, highlighting the importance of AK1 in maintaining cellular energy homeostasis and overall metabolic health. AK1 (G-9) antibody serves as an invaluable tool for researchers studying energy metabolism and related disorders, providing reliable detection and analysis of this critical enzyme.

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AK1 Antibody (G-9) References:

1. Adenylate kinase I does not affect cellular growth characteristics under normal and metabolic stress conditions.  |  de Bruin, W., et al. 2004. Exp Cell Res. 297: 97-107. PMID: 15194428
2. A radiation-reduced hybrid cell line containing 5 Mb/17 cM of human DNA from 9q34.  |  Henske, EP., et al. 1992. Genomics. 13: 841-4. PMID: 1639411
3. Induction of in vivo synthetic lethal RNAi responses to treat glioblastoma.  |  Michiue, H., et al. 2009. Cancer Biol Ther. 8: 2306-13. PMID: 19901546
4. Comparison of the substrate selectivity and biochemical properties of human and bacterial γ-butyrobetaine hydroxylase.  |  Rydzik, AM., et al. 2014. Org Biomol Chem. 12: 6354-8. PMID: 25030770
5. Antigen-specific recognition of autologous leukemia cells and allogeneic class-I MHC antigens by IL-2-activated cytotoxic T cells from a patient with acute T-cell leukemia.  |  Fisch, P., et al. 1989. Blood. 74: 343-53. PMID: 2546621
6. Evidence for genetic heterogeneity in tuberous sclerosis.  |  Sampson, JR., et al. 1989. J Med Genet. 26: 511-6. PMID: 2769723
7. Key miRNAs and target genes played roles in the development of clear cell renal cell carcinoma.  |  Liu, J., et al. 2018. Cancer Biomark. 23: 279-290. PMID: 30198869
8. Clonal origin of the Philadelphia translocation in chronic myeloid leukemia demonstrated in somatic cell hybrids using an adenylate kinase-1 polymorphism.  |  Geurts van Kessel, AH., et al. 1982. Cancer Genet Cytogenet. 6: 55-8. PMID: 6286097
9. Distinction of seventy-one cultured human tumor cell lines by polymorphic enzyme analysis.  |  Wright, WC., et al. 1981. J Natl Cancer Inst. 66: 239-47. PMID: 6935474
10. Increase of adenylate kinase isozyme 1 protein during neuronal differentiation in mouse embryonal carcinoma P19 cells and in rat brain primary cultured cells.  |  Inouye, S., et al. 1998. J Neurochem. 71: 125-33. PMID: 9648858

The preferred AK1 antibody is AK1 Antibody (E-8): sc-365316.