Ajuba Antibody (F-9): sc-514001

Ajuba Antibody (F-9) is a mouse monoclonal IgG1 antibody that detects Ajuba in mouse, rat, and human samples through applications such as western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). Ajuba, a member of the Zyxin family of LIM proteins, plays a crucial role in various cellular processes, particularly in cell signaling pathway regulation. Ajuba (F-9) antibody helps reveal its involvement in the IL-1 signaling pathway, where Ajuba modulates interleukin-1-induced NFκB activation by influencing the assembly and activity of the αPKC/p62/TRAF6 multiprotein signaling complex. This regulation is vital for controlling inflammatory responses and cellular stress, highlighting Ajuba′s importance in maintaining cellular homeostasis. Additionally, Ajuba is essential for cadherin-mediated cell-cell adhesion and influences cell migration by regulating the synthesis of phosphatidylinositol 4,5-bisphosphate (PIP2) through direct activation of PIPKIα activity. In differentiating mouse embryonic stem cells, Ajuba transcription is notably elevated, indicating its role in development. In adult mouse tissues, Ajuba is expressed in the skin, brain, and genitourinary organs, with immunofluorescence (IF) analysis revealing cytoplasmic localization in unsynchronized HeLa cells. Notably, during the G2/M phase of the cell cycle, Ajuba localizes to the centrosome, where Ajuba interacts with LATS2 to regulate spindle apparatus organization by recruiting γ Tubulin, further underscoring its critical role in cell division and organization.

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Ajuba Antibody (F-9) References:

1. Ajuba functions as a histone deacetylase-dependent co-repressor for autoregulation of the growth factor-independent-1 transcription factor.  |  Montoya-Durango, DE., et al. 2008. J Biol Chem. 283: 32056-65. PMID: 18805794
2. The LIM protein Ajuba promotes adipogenesis by enhancing PPARγ and p300/CBP interaction.  |  Li, Q., et al. 2016. Cell Death Differ. 23: 158-68. PMID: 26113042
3. Ajuba Preferentially Binds LXRα/RXRγ Heterodimer to Enhance LXR Target Gene Expression in Liver Cells.  |  Fan, H., et al. 2015. Mol Endocrinol. 29: 1608-18. PMID: 26389695
4. Ajuba Phosphorylation by CDK1 Promotes Cell Proliferation and Tumorigenesis.  |  Chen, X., et al. 2016. J Biol Chem. 291: 14761-72. PMID: 27226586
5. The scaffold protein Ajuba suppresses CdGAP activity in epithelia to maintain stable cell-cell contacts.  |  McCormack, JJ., et al. 2017. Sci Rep. 7: 9249. PMID: 28835688
6. The force-sensitive protein Ajuba regulates cell adhesion during epithelial morphogenesis.  |  Razzell, W., et al. 2018. J Cell Biol. 217: 3715-3730. PMID: 30006462
7. Super-enhancer-driven AJUBA is activated by TCF4 and involved in epithelial-mesenchymal transition in the progression of Hepatocellular Carcinoma.  |  Zhang, C., et al. 2020. Theranostics. 10: 9066-9082. PMID: 32802179
8. Knockout of Ajuba Attenuates the Growth and Migration of Hepatocellular Carcinoma Cells.  |  Le, Y., et al. 2020. Cytogenet Genome Res. 160: 650-658. PMID: 33640888
9. The LIM Protein AJUBA is a Potential Oncogenic Target and Prognostic Marker in Human Cancer via Pan-Cancer Analysis.  |  Song, N., et al. 2022. Front Cell Dev Biol. 10: 921897. PMID: 35898403
10. Evaluation of Immunoexpression of AJUBA Protein in Normal Oral Mucosa and Oral Squamous Cell Carcinoma.  |  Desai, P., et al. 2023. Appl Immunohistochem Mol Morphol. 31: 1-8. PMID: 36222508

The preferred Ajuba antibody is Ajuba Antibody (B-3): sc-398008.