Ajuba Antibody (B-3): sc-398008

Ajuba Antibody (B-3) is a mouse monoclonal IgG1 kappa light chain antibody that detects Ajuba protein of mouse, rat, and human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). Anti-Ajuba antibody (B-3) is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. Ajuba, a member of the Zyxin family of LIM proteins, plays a crucial role in mediating cellular events, particularly in the IL-1 signaling pathway, where Ajuba modulates interleukin-1-induced NFkB activation. This modulation occurs through Ajuba′s influence on the assembly and activity of the αPKC/p62/TRAF6 multiprotein signaling complex, highlighting Ajuba′s importance in inflammatory responses. Ajuba is involved in cadherin-mediated cell-cell adhesion and regulates cell migration by directly activating PIPKIα, which is essential for the synthesis of phosphatidylinositol 4,5-bisphosphate (PI(4,5)P2). Elevated transcription of Ajuba is observed in differentiating mouse embryonic stem cells, indicating Ajuba′s role in development. In adult mouse tissues, Ajuba is expressed in the skin, brain, and genitourinary organs, with immunofluorescence (IF) analysis revealing cytoplasmic localization in unsynchronized HeLa cells. During the G2/M phase of the cell cycle, Ajuba localizes to the centrosome, where Ajuba interacts with LATS2 to regulate spindle apparatus organization through the recruitment of γ Tubulin, underscoring Ajuba′s significance in cell division and maintaining genomic stability.

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Ajuba Antibody (B-3) References:

1. Ajuba, a novel LIM protein, interacts with Grb2, augments mitogen-activated protein kinase activity in fibroblasts, and promotes meiotic maturation of Xenopus oocytes in a Grb2- and Ras-dependent manner.  |  Goyal, RK., et al. 1999. Mol Cell Biol. 19: 4379-89. PMID: 10330178
2. The amino terminus of the glial glutamate transporter GLT-1 interacts with the LIM protein Ajuba.  |  Marie, H., et al. 2002. Mol Cell Neurosci. 19: 152-64. PMID: 11860269
3. The LIM protein Ajuba is recruited to cadherin-dependent cell junctions through an association with alpha-catenin.  |  Marie, H., et al. 2003. J Biol Chem. 278: 1220-8. PMID: 12417594
4. The tumor suppressor Scrib interacts with the zyxin-related protein LPP, which shuttles between cell adhesion sites and the nucleus.  |  Petit, MM., et al. 2005. BMC Cell Biol. 6: 1. PMID: 15649318
5. The LIM protein Ajuba influences p130Cas localization and Rac1 activity during cell migration.  |  Pratt, SJ., et al. 2005. J Cell Biol. 168: 813-24. PMID: 15728191
6. Targeted proteomic analysis of 14-3-3 sigma, a p53 effector commonly silenced in cancer.  |  Benzinger, A., et al. 2005. Mol Cell Proteomics. 4: 785-95. PMID: 15778465
7. The LIM protein Ajuba influences interleukin-1-induced NF-kappaB activation by affecting the assembly and activity of the protein kinase Czeta/p62/TRAF6 signaling complex.  |  Feng, Y. and Longmore, GD. 2005. Mol Cell Biol. 25: 4010-22. PMID: 15870274
8. LATS2-Ajuba complex regulates gamma-tubulin recruitment to centrosomes and spindle organization during mitosis.  |  Abe, Y., et al. 2006. FEBS Lett. 580: 782-8. PMID: 16413547
9. Ajuba is required for Rac activation and maintenance of E-cadherin adhesion.  |  Nola, S., et al. 2011. J Cell Biol. 195: 855-71. PMID: 22105346
10. AJUBA increases the cisplatin resistance through hippo pathway in cervical cancer.  |  Bi, L., et al. 2018. Gene. 644: 148-154. PMID: 29126926
11. Ajuba inhibits hepatocellular carcinoma cell growth via targeting of β-catenin and YAP signaling and is regulated by E3 ligase Hakai through neddylation.  |  Liu, M., et al. 2018. J Exp Clin Cancer Res. 37: 165. PMID: 30041665
12. LIM protein Ajuba promotes liver cell proliferation through its involvement in DNA replication and DNA damage control.  |  Dommann, N., et al. 2022. FEBS Lett. 596: 1746-1764. PMID: 35535434

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-Ajuba Antibody (B-3) has 1 citations in a variety of scientific publications.

Primary antibodies like Anti-Ajuba Antibody (B-3) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

Ajuba Antibody (B-3) Specifications

Alternate Product Name: Ajuba

Product Clone Name: B-3

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG₁ kappa light chain

Amino Acids: 67-94

Epitope Target: Ajuba

Epitope Species: human

Product Reactivity/Detection: Ajuba

Species Reactivity/Detection: mouse, rat, human

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, ELISA

Vialing Information: Each vial contains 200 µg IgG₁ kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 1 citations in various scientific publications

Available Conjugates/Forms for Ajuba Antibody (B-3): AC: Agarose, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, FITC: Fluoroscein, HRP: Horseradish Peroxidase, P: Blocking Peptide, PE: Phycoerythrin