AID Antibody (H-3): sc-518170
- AID Antibody (H-3) is a mouse monoclonal IgG2b κ provided at 200 µg/ml
- specific for an epitope mapping between amino acids 116-134 of AID of human origin
- recommended for detection of AID of human origin by WB, IP, IF and ELISA
- Anti-AID Antibody (H-3) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
- also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- m-IgG2b BP-HRP and m-IgGκ BP-HRP are the preferred secondary detection reagents for AID Antibody (H-3) for WB applications. These reagents are now offered in bundles with AID Antibody (H-3) (see ordering information below).
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AID Antibody (H-3) is a mouse monoclonal IgG2b kappa light chain antibody that detects Activation-induced Cytidine Deaminase (AID) of human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). Anti-AID antibody (H-3) is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. Activation-induced Cytidine Deaminase is a crucial 198-amino acid RNA-editing enzyme that contains a conserved cytidine deaminase motif, playing a vital role in B-cell terminal differentiation. AID monoclonal antibody (H-3) targets proteins primarily located in germinal center B cells, where AID facilitates the production of neutralizing antibodies such as IgG, IgA, and IgE. The proper functioning of AID is essential for immunoglobulin class switch recombination (CSR) and somatic hypermutation, processes that are critical for generating a diverse and effective antibody response. Deficiencies in AID lead to Hyper-IgM syndrome (HIGM2), characterized by the absence of CSR and somatic hypermutations, resulting in lymph node hyperplasia and the presence of giant germinal centers. Additionally, AID-deficient mice exhibit a hyper-IgM phenotype, underscoring AID′s importance in multiple stages of B-cell differentiation necessary for robust immune responses.
Alexa Fluor® is a trademark of Molecular Probes Inc., OR., USA
LI-COR® and Odyssey® are registered trademarks of LI-COR Biosciences
AID Antibody (H-3) References:
- Specific expression of activation-induced cytidine deaminase (AID), a novel member of the RNA-editing deaminase family in germinal center B cells. | Muramatsu, M., et al. 1999. J Biol Chem. 274: 18470-6. PMID: 10373455
- Isolation, tissue distribution, and chromosomal localization of the human activation-induced cytidine deaminase (AID) gene. | Muto, T., et al. 2000. Genomics. 68: 85-8. PMID: 10950930
- Class switch recombination and hypermutation require activation-induced cytidine deaminase (AID), a potential RNA editing enzyme. | Muramatsu, M., et al. 2000. Cell. 102: 553-63. PMID: 11007474
- Activation-induced cytidine deaminase (AID) deficiency causes the autosomal recessive form of the Hyper-IgM syndrome (HIGM2). | Revy, P., et al. 2000. Cell. 102: 565-75. PMID: 11007475
- Mutations in activation-induced cytidine deaminase in patients with hyper IgM syndrome. | Minegishi, Y., et al. 2000. Clin Immunol. 97: 203-10. PMID: 11112359
- Activation-induced cytidine deaminase in antibody diversification and chromosome translocation. | Gazumyan, A., et al. 2012. Adv Cancer Res. 113: 167-90. PMID: 22429855
- Epigenetic targeting of activation-induced cytidine deaminase. | Wang, Q., et al. 2014. Proc Natl Acad Sci U S A. 111: 18667-72. PMID: 25512519
- Epigenetic function of activation-induced cytidine deaminase and its link to lymphomagenesis. | Dominguez, PM. and Shaknovich, R. 2014. Front Immunol. 5: 642. PMID: 25566255
- Activation-induced cytidine deaminase and active DNA demethylation. | Ramiro, AR. and Barreto, VM. 2015. Trends Biochem Sci. 40: 172-81. PMID: 25661247
- Activation-Induced Cytidine Deaminase Links Ovulation-Induced Inflammation and Serous Carcinogenesis. | Sapoznik, S., et al. 2016. Neoplasia. 18: 90-9. PMID: 26936395
- Activation-induced cytidine deaminase expression in colorectal cancer. | Li, L., et al. 2019. Int J Clin Exp Pathol. 12: 4119-4124. PMID: 31933808
- Activation-induced cytidine deaminase causes recurrent splicing mutations in diffuse large B-cell lymphoma. | Benitez-Cantos, MS., et al. 2024. Mol Cancer. 23: 42. PMID: 38402205
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
AID Antibody (H-3) | sc-518170 | 200 µg/ml | $322.00 | |||
AID Antibody (H-3): m-IgGκ BP-HRP Bundle | sc-550689 | 200 µg Ab; 40 µg BP | $361.00 | |||
AID Antibody (H-3): m-IgG2b BP-HRP Bundle | sc-550690 | 200 µg Ab; 10 µg BP | $361.00 | |||
AID Antibody (H-3) AC | sc-518170 AC | 500 µg/ml, 25% agarose | $424.00 | |||
AID Antibody (H-3) HRP | sc-518170 HRP | 200 µg/ml | $322.00 | |||
AID Antibody (H-3) FITC | sc-518170 FITC | 200 µg/ml | $336.00 | |||
AID Antibody (H-3) PE | sc-518170 PE | 200 µg/ml | $349.00 | |||
AID Antibody (H-3) Alexa Fluor® 488 | sc-518170 AF488 | 200 µg/ml | $364.00 | |||
AID Antibody (H-3) Alexa Fluor® 546 | sc-518170 AF546 | 200 µg/ml | $364.00 | |||
AID Antibody (H-3) Alexa Fluor® 594 | sc-518170 AF594 | 200 µg/ml | $364.00 | |||
AID Antibody (H-3) Alexa Fluor® 647 | sc-518170 AF647 | 200 µg/ml | $364.00 | |||
AID Antibody (H-3) Alexa Fluor® 680 | sc-518170 AF680 | 200 µg/ml | $364.00 | |||
AID Antibody (H-3) Alexa Fluor® 790 | sc-518170 AF790 | 200 µg/ml | $364.00 |