AHSP Antibody (G-5): sc-515189

AHSP Antibody (G-5) is a mouse monoclonal IgG2a kappa light chain antibody that detects α-hemoglobin stabilizing protein (AHSP) in mouse, rat, and human samples through applications such as western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). AHSP Antibody (G-5) is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. AHSP plays a crucial role in erythroid cell development by acting as a chaperone that prevents the aggregation of free α-hemoglobin, which is vital for maintaining hemoglobin stability and function. By forming a heterodimer with free α-hemoglobin, AHSP ensures that excess α-hemoglobin does not precipitate, thereby protecting erythroid cells from potential damage caused by hemoglobin misfolding. AHSP is primarily localized in the cytoplasm and is expressed in blood and bone marrow, where AHSP is essential for normal erythropoiesis. Notably, AHSP expression is downregulated in transmissible spongiform encephalopathies, indicating AHSP′s potential involvement in pathological conditions associated with α-hemoglobin excess, such as β-thalassemia, a hereditary disorder characterized by reduced β-globin chain synthesis.

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AHSP Antibody (G-5) References:

1. Cloning and functional analysis of cDNAs with open reading frames for 300 previously undefined genes expressed in CD34+ hematopoietic stem/progenitor cells.  |  Zhang, QH., et al. 2000. Genome Res. 10: 1546-60. PMID: 11042152
2. A novel erythroid-specific marker of transmissible spongiform encephalopathies.  |  Miele, G., et al. 2001. Nat Med. 7: 361-4. PMID: 11231637
3. An abundant erythroid protein that stabilizes free alpha-haemoglobin.  |  Kihm, AJ., et al. 2002. Nature. 417: 758-63. PMID: 12066189
4. Biophysical characterization of the alpha-globin binding protein alpha-hemoglobin stabilizing protein.  |  Gell, D., et al. 2002. J Biol Chem. 277: 40602-9. PMID: 12192002
5. Conformational response of tartaric acid to derivatization: role of 1,3-dipole-dipole interactions.  |  Gawronski, J., et al. 2005. Chirality. 17: 388-95. PMID: 15991225
6. AHSP and beta-thalassemia: a possible genetic modifier.  |  dos Santos, CO. and Costa, FF. 2005. Hematology. 10: 157-61. PMID: 16019463
7. GATA-1 and Oct-1 are required for expression of the human alpha-hemoglobin-stabilizing protein gene.  |  Gallagher, PG., et al. 2005. J Biol Chem. 280: 39016-23. PMID: 16186125

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-AHSP Antibody (G-5) has 0 citations in a variety of scientific publications.

Primary antibodies like Anti-AHSP Antibody (G-5) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

AHSP Antibody (G-5) Specifications

Alternate Product Name: AHSP

Product Clone Name: G-5

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG_2a kappa light chain

Amino Acids: 1-102

Epitope Target: AHSP

Epitope Species: mouse

Product Reactivity/Detection: AHSP

Species Reactivity/Detection: mouse, rat, human

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, ELISA

Vialing Information: Each vial contains 200 µg IgG_2a kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 0 citations in various scientific publications

Available Conjugates/Forms for AHSP Antibody (G-5): AC: Agarose, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, FITC: Fluoroscein, HRP: Horseradish Peroxidase, PE: Phycoerythrin

The preferred AHSP antibody is AHSP Antibody (D-12): sc-515436.