AHI1 Antibody (B-2): sc-515382

AHI1 Antibody (B-2) is a mouse monoclonal IgG1 kappa light chain antibody that detects AHI1 protein of mouse, rat, and human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). AHI1 (B-2) antibody is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. AHI1, also known as Abelson helper integration site 1, ORF1, AHI-1, JBTS3, or Jouberin, is a 1196 amino acid signaling protein predominantly expressed in the brain, particularly in neurons that contribute to the crossing axons of the corticospinal tract and superior cerebellar peduncles. AHI1′s structure contains seven WD repeats and an SH3 domain, which are essential for mediating protein-protein interactions and signaling pathways critical for cerebellar and cortical development. Disruptions in AHI1 function due to mutations are linked to Joubert syndrome, an autosomal recessive disorder that presents with hypotonia, ataxia, mental retardation, altered respiratory patterns, abnormal eye movements, and brain malformations. AHI1 is recognized as a novel oncogene, with significant deregulation observed in chronic myeloid leukemia, highlighting AHI1′s importance in both developmental biology and cancer research. Three isoforms of AHI1 arise from alternative splicing, adding complexity to AHI1′s functional roles in cellular processes.

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AHI1 Antibody (B-2) References:

1. Genome annotation of a 1.5 Mb region of human chromosome 6q23 encompassing a quantitative trait locus for fetal hemoglobin expression in adults.  |  Close, J., et al. 2004. BMC Genomics. 5: 33. PMID: 15169551
2. Mutations in the AHI1 gene, encoding jouberin, cause Joubert syndrome with cortical polymicrogyria.  |  Dixon-Salazar, T., et al. 2004. Am J Hum Genet. 75: 979-87. PMID: 15467982
3. AHI1 mutations cause both retinal dystrophy and renal cystic disease in Joubert syndrome.  |  Parisi, MA., et al. 2006. J Med Genet. 43: 334-9. PMID: 16155189
4. Joubert syndrome (and related disorders) (OMIM 213300).  |  Parisi, MA., et al. 2007. Eur J Hum Genet. 15: 511-21. PMID: 17377524
5. DNA analysis of AHI1, NPHP1 and CYCLIN D1 in Joubert syndrome patients from the Netherlands.  |  Kroes, HY., et al. 2008. Eur J Med Genet. 51: 24-34. PMID: 18054307
6. Association of common variants in the Joubert syndrome gene (AHI1) with autism.  |  Alvarez Retuerto, AI., et al. 2008. Hum Mol Genet. 17: 3887-96. PMID: 18782849
7. Species differences in the expression of Ahi1, a protein implicated in the neurodevelopmental disorder Joubert syndrome, with preferential accumulation to stigmoid bodies.  |  Doering, JE., et al. 2008. J Comp Neurol. 511: 238-56. PMID: 18785627
8. AHI-1 interacts with BCR-ABL and modulates BCR-ABL transforming activity and imatinib response of CML stem/progenitor cells.  |  Zhou, LL., et al. 2008. J Exp Med. 205: 2657-71. PMID: 18936234
9. Cloning and characterization of the promoter of the human AHI1 gene.  |  Meng, XF., et al. 2009. Biochem Genet. 47: 427-38. PMID: 19191019

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-AHI1 Antibody (B-2) has 5 citations in a variety of scientific publications.

Primary antibodies like Anti-AHI1 Antibody (B-2) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

AHI1 Antibody (B-2) Specifications

Alternate Product Name: AHI1

Product Clone Name: B-2

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG₁ kappa light chain

Amino Acids: 601-897

Epitope Target: AHI1

Epitope Species: human

Product Reactivity/Detection: AHI1

Species Reactivity/Detection: mouse, rat, human

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, ELISA

Vialing Information: Each vial contains 200 µg IgG₁ kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 5 citations in various scientific publications

Available Conjugates/Forms for AHI1 Antibody (B-2): AC: Agarose, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, FITC: Fluoroscein, HRP: Horseradish Peroxidase, PE: Phycoerythrin