AGS3 Antibody (G-2): sc-271721

AGS3 Antibody (G-2) is a mouse monoclonal IgG1 kappa light chain antibody that detects AGS3 of mouse, rat, and human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). AGS3 (G-2) antibody is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. AGS3 plays a crucial role in G-protein signaling pathways, acting as an activator of G-protein signaling that functions downstream of G-protein-coupled receptors (GPCRs) or at the level of heterotrimeric G-proteins. AGS3 is a Gai-binding protein that displaces Gβγ subunits and associates with Ga-GDP, stabilizing the GDP-bound conformation of Ga, which is essential for the regulation of various cellular processes. AGS3 is predominantly localized in the cytoplasm and is expressed in several cell types, including rat brain, PC12 cells, NG108-15 cells, and DDT(1)-MF2 smooth muscle cells. AGS3 exists in two forms: a longer 227 amino acid variant with seven tetratricopeptide repeat (TPR) domains, which are important for targeting proteins to specific subcellular regions, and a shorter 166 amino acid variant that is more prevalent in the adult rat heart. Understanding AGS3 localization and function is vital, as AGS3 contributes to the intricate signaling mechanisms that govern cellular responses in various physiological contexts.

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AGS3 Antibody (G-2) References:

1. Receptor-independent activators of heterotrimeric G-protein signaling pathways.  |  Takesono, A., et al. 1999. J Biol Chem. 274: 33202-5. PMID: 10559191
2. AGS3 inhibits GDP dissociation from galpha subunits of the Gi family and rhodopsin-dependent activation of transducin.  |  Natochin, M., et al. 2000. J Biol Chem. 275: 40981-5. PMID: 11024022
3. Selective interaction of AGS3 with G-proteins and the influence of AGS3 on the activation state of G-proteins.  |  Bernard, ML., et al. 2001. J Biol Chem. 276: 1585-93. PMID: 11042168
4. Activator of G protein signaling 3 is a guanine dissociation inhibitor for Galpha i subunits.  |  De Vries, L., et al. 2000. Proc Natl Acad Sci U S A. 97: 14364-9. PMID: 11121039
5. Identification of a truncated form of the G-protein regulator AGS3 in heart that lacks the tetratricopeptide repeat domains.  |  Pizzinat, N., et al. 2001. J Biol Chem. 276: 16601-10. PMID: 11278352
6. Receptor-independent activators of heterotrimeric G-proteins.  |  Cismowski, MJ., et al. 2001. Life Sci. 68: 2301-8. PMID: 11358340
7. Activator of G protein signaling 3: a gatekeeper of cocaine sensitization and drug seeking.  |  Bowers, MS., et al. 2004. Neuron. 42: 269-81. PMID: 15091342
8. A specific role of AGS3 in the surface expression of plasma membrane proteins.  |  Groves, B., et al. 2007. Proc Natl Acad Sci U S A. 104: 18103-8. PMID: 17991770
9. A role for activator of G-protein signaling 3 (AGS3) in multiple myeloma.  |  Shao, S., et al. 2014. Int J Hematol. 99: 57-68. PMID: 24307516
10. AGS3 is involved in TNF-α medicated osteogenic differentiation of human dental pulp stem cells.  |  Xing, J., et al. 2015. Differentiation. 89: 128-36. PMID: 26143356
11. AGS3-dependent trans-Golgi network membrane trafficking is essential for compaction in mouse embryos.  |  Nie, ZW., et al. 2020. J Cell Sci. 133: PMID: 33148610
12. AGS3 antagonizes LGN to balance oriented cell divisions and cell fate choices in mammalian epidermis.  |  Descovich, CP., et al. 2023. Elife. 12: PMID: 37017303

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-AGS3 Antibody (G-2) has 2 citations in a variety of scientific publications.

Primary antibodies like Anti-AGS3 Antibody (G-2) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

AGS3 Antibody (G-2) Specifications

Alternate Product Name: AGS3

Product Clone Name: G-2

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG₁ kappa light chain

Amino Acids: 346-470

Epitope Target: AGS3

Epitope Species: rat

Product Reactivity/Detection: AGS3

Species Reactivity/Detection: mouse, rat, human

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, ELISA

Vialing Information: Each vial contains 200 µg IgG₁ kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 2 citations in various scientific publications

Available Conjugates/Forms for AGS3 Antibody (G-2): AC: Agarose, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, FITC: Fluoroscein, HRP: Horseradish Peroxidase, PE: Phycoerythrin