AGRP Antibody (H-5): sc-518077

AGRP Antibody (H-5) is a mouse monoclonal IgG1 kappa light chain antibody that detects AGRP protein of mouse, rat, and human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). AGRP (H-5) antibody is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. AGRP, or agouti-related protein, plays a crucial role in energy homeostasis and appetite regulation by acting as a potent antagonist of melanocortin receptors MC3R and MC4R, which are involved in central regulation of body weight. This antagonistic action is vital for maintaining energy balance, as AGRP influences feeding behavior and energy expenditure. AGRP expression occurs primarily in adrenal gland, subthalamic nucleus, and hypothalamus, with lower levels found in testis, lung, and kidney, highlighting AGRP′s importance in neuroendocrine signaling pathways. AGRP gene is located on human chromosome 16q22, and AGRP expression remains tightly regulated, making AGRP a significant target for research into obesity and metabolic disorders.

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AGRP Antibody (H-5) References:

1. AgRP Neurons Require Carnitine Acetyltransferase to Regulate Metabolic Flexibility and Peripheral Nutrient Partitioning.  |  Reichenbach, A., et al. 2018. Cell Rep. 22: 1745-1759. PMID: 29444428
2. Control of Energy Expenditure by AgRP Neurons of the Arcuate Nucleus: Neurocircuitry, Signaling Pathways, and Angiotensin.  |  Morselli, LL., et al. 2018. Curr Hypertens Rep. 20: 25. PMID: 29556733
3. AgRP neurons control compulsive exercise and survival in an activity-based anorexia model.  |  Miletta, MC., et al. 2020. Nat Metab. 2: 1204-1211. PMID: 33106687
4. Insulin signalling in tanycytes gates hypothalamic insulin uptake and regulation of AgRP neuron activity.  |  Porniece Kumar, M., et al. 2021. Nat Metab. 3: 1662-1679. PMID: 34931084
5. Metabolic sensing in AgRP neurons integrates homeostatic state with dopamine signalling in the striatum.  |  Reichenbach, A., et al. 2022. Elife. 11: PMID: 35018884
6. AgRP neurons control feeding behaviour at cortical synapses via peripherally derived lysophospholipids.  |  Endle, H., et al. 2022. Nat Metab. 4: 683-692. PMID: 35760867
7. Lipid biosynthesis enzyme Agpat5 in AgRP-neurons is required for insulin-induced hypoglycemia sensing and glucagon secretion.  |  Strembitska, A., et al. 2022. Nat Commun. 13: 5761. PMID: 36180454
8. Growth hormone receptor (GHR) in AgRP neurons regulates thermogenesis in a sex-specific manner.  |  Stilgenbauer, L., et al. 2023. Geroscience. 45: 1745-1759. PMID: 36633824
9. Ghrelin signalling in AgRP neurons links metabolic state to the sensory regulation of AgRP neural activity.  |  So, WL., et al. 2023. Mol Metab. 78: 101826. PMID: 37898450
10. AgRP neurons mediate activity-dependent development of oxytocin connectivity and autonomic regulation.  |  Biddinger, JE., et al. 2024. Proc Natl Acad Sci U S A. 121: e2403810121. PMID: 39585985

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-AGRP Antibody (H-5) has 3 citations in a variety of scientific publications.

Primary antibodies like Anti-AGRP Antibody (H-5) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

AGRP Antibody (H-5) Specifications

Alternate Product Name: Agouti Related Protein

Product Clone Name: H-5

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG₁ kappa light chain

Amino Acids: 64-90

Epitope Target: AGRP

Epitope Species: mouse

Product Reactivity/Detection: AGRP

Species Reactivity/Detection: mouse, rat, human

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, ELISA

Vialing Information: Each vial contains 200 µg IgG₁ kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 3 citations in various scientific publications

Available Conjugates/Forms for AGRP Antibody (H-5): AC: Agarose, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, FITC: Fluoroscein, HRP: Horseradish Peroxidase, PE: Phycoerythrin

The preferred AGRP antibody is AGRP Antibody (MM0085-32B12): sc-517457.