aggrecan/brevican Antibody (D-4): sc-166951

Aggrecan/brevican Antibody (D-4) is a mouse monoclonal IgM kappa antibody that detects aggrecan/brevican from mouse, rat, and human samples through western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). Anti-aggrecan/brevican antibody (D-4) is available in non-conjugated form. Aggrecan, a large chondroitin sulfate proteoglycan, is the predominant proteoglycan found in cartilage and plays a crucial role in maintaining the structural integrity and resilience of cartilage tissue. Aggrecan belongs to the chondroitin sulfate proteoglycan family, which also includes versican, neurocan, and brevican. Aggrecan′s complex multidomain structure allows formation of aggregates with hyaluronan and link proteins, which are embedded within a collagen network, contributing to the compressive stiffness and resilience of hyaline cartilage. Degradation of aggrecan is often associated with various forms of inflammatory arthritis, leading to a loss of cartilage integrity. Brevican is primarily expressed in the brain and undergoes proteolytic cleavage in the adult brain, resulting in a full-length form and two fragments, which are essential for neural development and plasticity. Anti-aggrecan/brevican antibody (D-4) effectively detects these proteins, making this antibody a valuable tool for research into cartilage biology and neurological studies.

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aggrecan/brevican Antibody (D-4) References:

1. Extraction of aggrecan-peptide from cartilage by tissue autolysis.  |  Nakano, T., et al. 2014. Recent Pat Food Nutr Agric. 6: 54-9. PMID: 24923587
2. Structure, function, aging and turnover of aggrecan in the intervertebral disc.  |  Sivan, SS., et al. 2014. Biochim Biophys Acta. 1840: 3181-9. PMID: 25065289
3. ADAMTS4 and ADAMTS5 knockout mice are protected from versican but not aggrecan or brevican proteolysis during spinal cord injury.  |  Demircan, K., et al. 2014. Biomed Res Int. 2014: 693746. PMID: 25101296
4. Activity-Dependent Gating of Parvalbumin Interneuron Function by the Perineuronal Net Protein Brevican.  |  Favuzzi, E., et al. 2017. Neuron. 95: 639-655.e10. PMID: 28712654
5. Noise exposure alters MMP9 and brevican expression in the rat primary auditory cortex.  |  Park, SS., et al. 2020. BMC Neurosci. 21: 16. PMID: 32334536
6. Aggrecan in Cardiovascular Development and Disease.  |  Koch, CD., et al. 2020. J Histochem Cytochem. 68: 777-795. PMID: 32870742
7. FMRP regulation of aggrecan mRNA translation controls perineuronal net development.  |  Van't Spijker, HM. and Richter, JD. 2024. J Neurochem. 168: 1909-1922. PMID: 38225196
8. Serum Brevican as a Biomarker of Cerebrovascular Disease in an Elderly Cognitively Impaired Cohort.  |  Chia, RSL., et al. 2024. Biomolecules. 14: PMID: 38254675
9. The Perineuronal Net Protein Brevican Acts in Nucleus Accumbens Parvalbumin-Expressing Interneurons of Adult Mice to Regulate Excitatory Synaptic Inputs and Motivated Behaviors.  |  Hazlett, MF., et al. 2024. Biol Psychiatry. 96: 694-707. PMID: 38346480
10. Brevican, a chondroitin sulfate proteoglycan of rat brain, occurs as secreted and cell surface glycosylphosphatidylinositol-anchored isoforms.  |  Seidenbecher, CI., et al. 1995. J Biol Chem. 270: 27206-12. PMID: 7592978

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-aggrecan/brevican Antibody (D-4) has 15 citations in a variety of scientific publications.

Primary antibodies like Anti-aggrecan/brevican Antibody (D-4) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

aggrecan/brevican Antibody (D-4) Specifications

Alternate Product Name: ACAN / BCAN

Product Clone Name: D-4

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgM kappa light chain

Amino Acids: 2261-2280

Epitope Target: aggrecan/brevican

Epitope Species: human

Product Reactivity/Detection: aggrecan and brevican

Species Reactivity/Detection: mouse, rat, human

Additional Mammalian Reactivity: canine

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, ELISA

Vialing Information: Each vial contains 200 µg IgM kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 15 citations in various scientific publications

Available Conjugates/Forms for aggrecan/brevican Antibody (D-4): P: Blocking Peptide