aggrecan/brevican Antibody (D-4) recommended for detection of aggrecan and brevican of mouse, rat and human origin by WB, IP, IF and ELISA

aggrecan/brevican Antibody (D-4): sc-166951

aggrecan/brevican Antibody (D-4) is rated 4.2 out of 5 by 5.
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Datasheets

    • aggrecan/brevican Antibody (D-4) is a mouse monoclonal IgM (kappa light chain) provided at 200 µg/ml
    • specific for an epitope mapping between amino acids 2261-2280 near the C-terminus of aggrecan/brevican of human origin
    • recommended for detection of aggrecan and brevican of mouse, rat and human origin by WB, IP, IF and ELISA; also reactive with additional species, including canine
    • blocking peptide, sc-166951 P
    • See m-IgGκ BP-HRP (mouse IgGκ binding protein-HRP), our highly recommended recombinant alternative to conventional secondary anti-mouse IgG reagents.
    • See aggrecan (4F4): sc-33695 for aggrecan/brevican antibody conjugates, including AC, HRP, FITC, PE, Alexa Fluor® 488, 594, 647, 680 and 790.
    • Contact our Technical Service Department (or your local Distributor) for more information on how to receive a FREE 10 µg sample of aggrecan/brevican (D-4): sc-166951.
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What application is the blocking peptide sc-166951 P appropriate for?

Asked by: jenni
Thank you for your question. The blocking peptide is intended for use as a negative control, by pre-adsorbing the mouse monoclonal antibody against the antigen. For full protocol details, please contact our Technical Services Department or view our online protocol here: https://www.scbt.com/scbt/resources/protocols/peptide-neutralization
Answered by: Technical Support
Date published: 2017-02-25
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Rated 5 out of 5 by from Nice cleavage bands in murine hippocampal lysates This antibody worked well in Western blot using 30 micrograms of murine hippocampal lysates, and an increase in cleavage products was observed with specific treatments- We used 1/1000 dilution which was more than enough.
Date published: 2017-09-06
Rated 4 out of 5 by from Band seen in WB using human skeletal muscle Band seen in WB using human skeletal muscle -SCBT QC
Date published: 2015-03-28
Rated 3 out of 5 by from Robust bands with background seen in WB using 293T Robust bands with background seen in WB using 293T, C6 and HeLa whole cell lysates. -SCBT QC
Date published: 2015-03-22
Rated 4 out of 5 by from Good band detected in WB using H4 whole cell Good band detected in WB using H4 whole cell lysate. -SCBT QC
Date published: 2015-02-07
Rated 5 out of 5 by from WB publishable data with rat tendon WB publishable data with rat tendon - SCBT Publication Review
Date published: 2013-01-20
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