aggrecan Antibody (4F4) recommended for detection of aggrecan of human origin by WB, IP, IF and IHC(P)

aggrecan Antibody (4F4): sc-33695

aggrecan Antibody (4F4) is rated 4.5 out of 5 by 13.
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Datasheets

    • Anti-aggrecan Antibody (4F4) is a mouse monoclonal IgG1 (kappa light chain) aggrecan antibody provided at 200 µg/ml
    • raised against human articular cartilage aggrecan
    • aggrecan Antibody (4F4) is recommended for detection of aggrecan of human origin by WB, IP, IF and IHC(P)
    • Anti-aggrecan Antibody (4F4) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
    • also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
    • also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
    • See m-IgGκ BP-HRP (mouse IgGκ binding protein-HRP), our highly recommended recombinant alternative to conventional secondary anti-mouse IgG reagents.
    • Contact our Technical Service Department (or your local Distributor) for more information on how to receive a FREE 10 µg sample of aggrecan (4F4): sc-33695.
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Hi there! Has this antibody been used in brain/spinal cord tissue for IHC?

Asked by: shantan
Thank you for your question. We have many citations of this antibody in IF and IHC, but unfortunately I do not see if any of them are in brain/spinal cord. Aggrecan is detected in chondrocytes throughout the developing skeleton/ cartilage and we have many publications in bone marrow.
Answered by: Tech Service
Date published: 2019-11-19

Can aggrecan (4F4) sc-33695 monoclonal antibody be used with porcine tissue or cells?

Asked by: Vanessa_silos
Thank you for your question. We have not tested this antibody in porcine tissue. We are aware of one product citation using this antibody in porcine tissue. Please refer to the following publication for details and verification, PMID: 19112334.
Answered by: Technical Support
Date published: 2019-04-13

I would like to detection of aggrecan in western blot assay. I tried dilution 1:500 in 5% skinmilk. and 2nd antibody used sc-2005 product. but It does not detection. How can I do? my sample orign is human pellet.

Asked by: vtml
Thank you for your question. We recommend starting with a 1:100 primary antibody dilution for Western blot. For further assistance, please contact Technical Service.
Answered by: Technical Support
Date published: 2018-12-06

Can aggrecan (4F4): sc-33695 monoclonal antibody be used in IF or IHC with mouse tissue or cells? Will there be non-specific staining?

Asked by: Trav11
Thank you for your inquiry. The use of mouse monoclonal antibodies with mouse samples is very common and typically poses no problem. To eliminate any potential cross-reactivity of an anti-mouse secondary detection reagent, aggrecan (4F4): sc-33695 is available in a variety of direct conjugations, such as HRP, PE, FITC and AlexaFluors.
Answered by: Technical Support
Date published: 2019-02-28
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Rated 4 out of 5 by from Good, it can be used in IHC I used in bone in 1:100. it's specific. I think it can used in 1:200.
Date published: 2017-08-09
Rated 4 out of 5 by from Good antibody in detecting aggrecan This antibody works very well in detecting cell cycle protein aggrecan without any nonspecific bands.
Date published: 2017-06-01
Rated 5 out of 5 by from Great antibody. Antibody provides clear and specific results.Great antibody.
Date published: 2017-02-13
Rated 5 out of 5 by from Excellent aggrecan antibody antibody is really great. we got specific signal for the protein at the right molecular weight.
Date published: 2017-02-08
Rated 5 out of 5 by from The use of this antibody was satisfactory The use of this antibody has a good specificity for WB dilution and the 1:500 results are perfect.
Date published: 2017-02-05
Rated 5 out of 5 by from Good, versatile antibody The aggrecan Antibody (4F4): sc-33695 works well for multiple applications including WB, IP, IF and IHC(P). This antibody has also been cited several times for further verification.
Date published: 2017-01-20
Rated 5 out of 5 by from Performance good antibody This antibody in the use of performance is very good, it produces a good effect in mice.
Date published: 2016-12-21
Rated 5 out of 5 by from Fantastic antibody of Agg! I have used many kinds of Agg from different companies, but unfortunately, some of them are not work well... But this Agg can easily get strong band at 1:1000! Beyond my imagination!
Date published: 2016-12-19
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