aggrecan Antibody (4F4): sc-33695
- aggrecan Antibody (4F4) is a mouse monoclonal IgG1 κ aggrecan antibody, cited in 40 publications, provided at 200 µg/ml
- raised against human articular cartilage aggrecan
- aggrecan Antibody (4F4) is recommended for detection of aggrecan of human origin by WB, IP, IF and IHC(P)
- Anti-aggrecan Antibody (4F4) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
- also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- m-IgG Fc BP-HRP and m-IgG1 BP-HRP are the preferred secondary detection reagents for aggrecan Antibody (4F4) for WB and IHC(P) applications. These reagents are now offered in bundles with aggrecan Antibody (4F4) (see ordering information below).
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aggrecan Antibody (4F4) is a mouse monoclonal IgG1 kappa light chain antibody that detects the aggrecan protein of human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and immunohistochemistry. anti-aggrecan antibody (4F4) is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. aggrecan protein is a large chondroitin sulfate proteoglycan that plays a crucial role in maintaining the structural integrity and resilience of cartilage. aggrecan protein is primarily located in the extracellular matrix of cartilage, where aggrecan forms aggregates with hyaluronan and link proteins, contributing to the tissue′s compressive strength and ability to withstand mechanical stress. This localization is vital for proper cartilage functioning, as aggrecan interacts with collagen fibers, providing necessary support and cushioning in joints. Notably, aggrecan degradation is often associated with various forms of inflammatory arthritis, leading to cartilage damage and loss, which underscores the significance of monitoring aggrecan levels in clinical settings.
Alexa Fluor® is a trademark of Molecular Probes Inc., OR., USA
LI-COR® and Odyssey® are registered trademarks of LI-COR Biosciences
aggrecan Antibody (4F4) References:
- Role of the C-terminal G3 domain in sorting and secretion of aggrecan core protein and ubiquitin-mediated degradation of accumulated mutant precursors. | Domowicz, MS., et al. 2000. J Biol Chem. 275: 35098-105. PMID: 11063750
- Aggrecan domains expected to traffic through the exocytic pathway are misdirected to the nucleus. | Chen, TL., et al. 2001. Exp Cell Res. 263: 224-35. PMID: 11161721
- Roles of aggrecan domains in biosynthesis, modification by glycosaminoglycans and product secretion. | Kiani, C., et al. 2001. Biochem J. 354: 199-207. PMID: 11171095
- Aggrecan: A Target Molecule of Autoimmune Reactions. | Buzás, EI., et al. 1996. Pathol Oncol Res. 2: 219-228. PMID: 11173607
- Cartilage proteoglycans. | Knudson, CB. and Knudson, W. 2001. Semin Cell Dev Biol. 12: 69-78. PMID: 11292372
- Aggrecan-based extracellular matrix is an integral part of the human basal ganglia circuit. | Brückner, G., et al. 2008. Neuroscience. 151: 489-504. PMID: 18055126
- Extraction of aggrecan-peptide from cartilage by tissue autolysis. | Nakano, T., et al. 2014. Recent Pat Food Nutr Agric. 6: 54-9. PMID: 24923587
- Aggrecan Directs Extracellular Matrix-Mediated Neuronal Plasticity. | Rowlands, D., et al. 2018. J Neurosci. 38: 10102-10113. PMID: 30282728
- Aggrecan in Cardiovascular Development and Disease. | Koch, CD., et al. 2020. J Histochem Cytochem. 68: 777-795. PMID: 32870742
- Aggrecan: Approaches to Study Biophysical and Biomechanical Properties. | Nia, HT., et al. 2022. Methods Mol Biol. 2303: 209-226. PMID: 34626381
- FMRP regulation of aggrecan mRNA translation controls perineuronal net development. | Van't Spijker, HM. and Richter, JD. 2024. J Neurochem. 168: 1909-1922. PMID: 38225196
- A plastic aggrecan barrier modulated by peripheral energy state gates metabolic signal access to arcuate neurons. | Kuczynski-Noyau, L., et al. 2024. Nat Commun. 15: 6701. PMID: 39112471
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
aggrecan Antibody (4F4) | sc-33695 | 200 µg/ml | $316.00 | |||
aggrecan Antibody (4F4): m-IgG Fc BP-HRP Bundle | sc-526707 | 200 µg Ab; 10 µg BP | $354.00 | |||
aggrecan Antibody (4F4): m-IgG1 BP-HRP Bundle | sc-532080 | 200 µg Ab; 20 µg BP | $354.00 | |||
aggrecan Antibody (4F4) AC | sc-33695 AC | 500 µg/ml, 25% agarose | $416.00 | |||
aggrecan Antibody (4F4) HRP | sc-33695 HRP | 200 µg/ml | $316.00 | |||
aggrecan Antibody (4F4) FITC | sc-33695 FITC | 200 µg/ml | $330.00 | |||
aggrecan Antibody (4F4) PE | sc-33695 PE | 200 µg/ml | $343.00 | |||
aggrecan Antibody (4F4) Alexa Fluor® 488 | sc-33695 AF488 | 200 µg/ml | $357.00 | |||
aggrecan Antibody (4F4) Alexa Fluor® 546 | sc-33695 AF546 | 200 µg/ml | $357.00 | |||
aggrecan Antibody (4F4) Alexa Fluor® 594 | sc-33695 AF594 | 200 µg/ml | $357.00 | |||
aggrecan Antibody (4F4) Alexa Fluor® 647 | sc-33695 AF647 | 200 µg/ml | $357.00 | |||
aggrecan Antibody (4F4) Alexa Fluor® 680 | sc-33695 AF680 | 200 µg/ml | $357.00 | |||
aggrecan Antibody (4F4) Alexa Fluor® 790 | sc-33695 AF790 | 200 µg/ml | $357.00 |