AFX1 Antibody (A-7): sc-373877

AFX1 Antibody (A-7) is a mouse monoclonal IgG2a kappa light chain antibody that detects AFX1 protein of mouse, rat, and human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), immunohistochemistry, and enzyme-linked immunosorbent assay (ELISA). AFX1 Antibody (A-7) is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. AFX1, also known as FoxO4, plays a crucial role in regulating cellular processes such as apoptosis, cell cycle progression, and oxidative stress response, making AFX1 vital for maintaining cellular homeostasis and preventing tumorigenesis. AFX1 is predominantly located in the nucleus, where AFX1 functions as a transcription factor, binding to specific DNA sequences to modulate gene expression. AFX1 nuclear localization is tightly regulated by post-translational modifications, particularly phosphorylation by the serine/threonine kinase Akt1, which sequesters AFX1 in the cytosol, thereby inhibiting transcriptional activity. Dysregulation of AFX1 has been implicated in various cancers, including acute lymphocytic leukemia and alveolar rhabdomyosarcoma, highlighting AFX1′s importance as a potential therapeutic target.

Alexa Fluor^® is a trademark of Molecular Probes Inc., OR., USA

LI-COR^® and Odyssey^® are registered trademarks of LI-COR Biosciences

AFX1 Antibody (A-7) References:

1. Akt promotes cell survival by phosphorylating and inhibiting a Forkhead transcription factor.  |  Brunet, A., et al. 1999. Cell. 96: 857-68. PMID: 10102273
2. Direct control of the Forkhead transcription factor AFX by protein kinase B.  |  Kops, GJ., et al. 1999. Nature. 398: 630-4. PMID: 10217147
3. Unspliced XBP1 Confers VSMC Homeostasis and Prevents Aortic Aneurysm Formation via FoxO4 Interaction.  |  Zhao, G., et al. 2017. Circ Res. 121: 1331-1345. PMID: 29089350
4. Inhibition of Brd4 alleviates renal ischemia/reperfusion injury-induced apoptosis and endoplasmic reticulum stress by blocking FoxO4-mediated oxidative stress.  |  Liu, H., et al. 2019. Redox Biol. 24: 101195. PMID: 31004990
5. Clinicopathological Significance of FOXO4 Expression and Correlation with Prx1 in Head and Neck Squamous Cell Carcinoma.  |  Lu, Y., et al. 2021. Anal Cell Pathol (Amst). 2021: 5510753. PMID: 34055579
6. The FoxO4/DKK3 axis represses IFN-γ expression by Th1 cells and limits antimicrobial immunity.  |  Chen, X., et al. 2022. J Clin Invest. 132: PMID: 36106640
7. Multiomic analysis implicates FOXO4 in genetic regulation of chick lens fiber cell differentiation.  |  Brennan, L., et al. 2023. Dev Biol. 504: 25-37. PMID: 37722500
8. Cloning and characterization of the t(X;11) breakpoint from a leukemic cell line identify a new member of the forkhead gene family.  |  Parry, P., et al. 1994. Genes Chromosomes Cancer. 11: 79-84. PMID: 7529552
9. Acute leukemias of different lineages have similar MLL gene fusions encoding related chimeric proteins resulting from chromosomal translocation.  |  Corral, J., et al. 1993. Proc Natl Acad Sci U S A. 90: 8538-42. PMID: 8378328
10. Structural characterization of the FKHR gene and its rearrangement in alveolar rhabdomyosarcoma.  |  Davis, RJ., et al. 1995. Hum Mol Genet. 4: 2355-62. PMID: 8634710
11. AFX1 and p54nrb: fine mapping, genomic structure, and exclusion as candidate genes of X-linked dystonia parkinsonism.  |  Peters, U., et al. 1997. Hum Genet. 100: 569-72. PMID: 9341872
12. Cloning and characterization of three human forkhead genes that comprise an FKHR-like gene subfamily.  |  Anderson, MJ., et al. 1998. Genomics. 47: 187-99. PMID: 9479491

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-AFX1 Antibody (A-7) has 5 citations in a variety of scientific publications.

Primary antibodies like Anti-AFX1 Antibody (A-7) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

AFX1 Antibody (A-7) Specifications

Alternate Product Name: AFX1

Product Clone Name: A-7

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG_2a kappa light chain

Amino Acids: 52-81

Epitope Target: AFX1

Epitope Species: mouse

Product Reactivity/Detection: AFX1

Species Reactivity/Detection: mouse, rat, human

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, IHC(P), ELISA

Vialing Information: Each vial contains 200 µg IgG_2a kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 5 citations in various scientific publications

Available Conjugates/Forms for AFX1 Antibody (A-7): AC: Agarose, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, FITC: Fluoroscein, HRP: Horseradish Peroxidase, P: Blocking Peptide, PE: Phycoerythrin, X: TransCruz Reagent