AFAP-110 Antibody (D-11): sc-374655

AFAP-110 Antibody (D-11) is a mouse monoclonal IgG1 kappa light chain antibody that detects AFAP-110 of mouse, rat, and human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). AFAP-110 (D-11) antibody is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. The actin filament-associated protein (AFAP-110) plays a crucial role in cellular signaling and organization by interacting directly with actin filaments through its C-terminal actin-binding domain. AFAP-110 contains additional protein-binding domains, functioning as an adaptor that links signaling molecules to actin filaments, thereby facilitating the formation of larger signaling complexes. AFAP-110 activates Src kinases in response to cellular signals and influences actin organization as a cross-linking protein. The structural integrity of AFAP-110 is vital, as deletion of specific binding elements, such as the leucine zipper motif, can lead to significant alterations in actin phenotypes, including repositioning of actin into rosettes. These changes in actin cytoskeletal conformations are critical, as they can inhibit cell division and movement, making AFAP-110 a potential therapeutic target for controlling tumorigenesis and metastasis.

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AFAP-110 Antibody (D-11) References:

1. The carboxy terminus of AFAP-110 modulates direct interactions with actin filaments and regulates its ability to alter actin filament integrity and induce lamellipodia formation.  |  Qian, Y., et al. 2000. Exp Cell Res. 255: 102-13. PMID: 10666339
2. The actin filament-associated protein AFAP-110 is an adaptor protein that modulates changes in actin filament integrity.  |  Baisden, JM., et al. 2001. Oncogene. 20: 6435-47. PMID: 11607843
3. The intrinsic ability of AFAP-110 to alter actin filament integrity is linked with its ability to also activate cellular tyrosine kinases.  |  Baisden, JM., et al. 2001. Oncogene. 20: 6607-16. PMID: 11641786
4. The actin cytoskeleton as a therapeutic target: state of the art and future directions.  |  Giganti, A. and Friederich, E. 2003. Prog Cell Cycle Res. 5: 511-25. PMID: 14593746
5. Analysis of the role of the leucine zipper motif in regulating the ability of AFAP-110 to alter actin filament integrity.  |  Qian, Y., et al. 2004. J Cell Biochem. 91: 602-20. PMID: 14755689
6. Protein kinase Calpha activates c-Src and induces podosome formation via AFAP-110.  |  Gatesman, A., et al. 2004. Mol Cell Biol. 24: 7578-97. PMID: 15314167
7. AFAP-110 is required for actin stress fiber formation and cell adhesion in MDA-MB-231 breast cancer cells.  |  Dorfleutner, A., et al. 2007. J Cell Physiol. 213: 740-9. PMID: 17520695
8. AFAP-110 is overexpressed in prostate cancer and contributes to tumorigenic growth by regulating focal contacts.  |  Zhang, J., et al. 2007. J Clin Invest. 117: 2962-73. PMID: 17885682
9. The integrity of the SH3 binding motif of AFAP-110 is required to facilitate tyrosine phosphorylation by, and stable complex formation with, Src.  |  Guappone, AC. and Flynn, DC. 1997. Mol Cell Biochem. 175: 243-52. PMID: 9350057
10. Src can regulate carboxy terminal interactions with AFAP-110, which influence self-association, cell localization and actin filament integrity.  |  Qian, Y., et al. 1998. Oncogene. 16: 2185-95. PMID: 9619827
11. Formation of a stable src-AFAP-110 complex through either an amino-terminal or a carboxy-terminal SH2-binding motif.  |  Guappone, AC., et al. 1998. Mol Carcinog. 22: 110-9. PMID: 9655255

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-AFAP-110 Antibody (D-11) has 2 citations in a variety of scientific publications.

Primary antibodies like Anti-AFAP-110 Antibody (D-11) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

AFAP-110 Antibody (D-11) Specifications

Alternate Product Name: AFAP-110

Product Clone Name: D-11

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG₁ kappa light chain

Amino Acids: 626-690

Epitope Target: AFAP-110

Epitope Species: human

Product Reactivity/Detection: AFAP-110

Species Reactivity/Detection: mouse, rat, human

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, ELISA

Vialing Information: Each vial contains 200 µg IgG₁ kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 2 citations in various scientific publications

Available Conjugates/Forms for AFAP-110 Antibody (D-11): AC: Agarose, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, FITC: Fluoroscein, HRP: Horseradish Peroxidase, PE: Phycoerythrin