AdoMetDC Antibody (E-6): sc-166970

AdoMetDC Antibody (E-6) is a mouse monoclonal IgG1 kappa light chain antibody that detects AdoMetDC protein of mouse, rat, and human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). anti-AdoMetDC antibody (E-6) is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. AdoMetDC, or adenosylmethionine decarboxylase 1, is a crucial enzyme in the polyamine biosynthesis pathway, which plays a significant role in cellular growth, proliferation, and tumor promotion. This 334 amino acid protein is synthesized as an inactive proenzyme that undergoes self-maturation to produce two non-identical subunits, α and β, which then assemble into an active heterotetramer composed of two α chains and two β chains. AdoMetDC utilizes a pyruvoyl group as a cofactor to convert S-adenosyl-L-methionine into (5-deoxy-5-adenosyl)(3-aminopropyl)-methylsulfonium salt and carbon dioxide. Processing of the AdoMetDC proenzyme and catalytic activity are stimulated by putrescine, while iodoacetic acid serves as an inhibitor, highlighting the regulatory mechanisms that control polyamine synthesis and their implications in cellular functions and cancer biology.

Alexa Fluor^® is a trademark of Molecular Probes Inc., OR., USA

LI-COR^® and Odyssey^® are registered trademarks of LI-COR Biosciences

AdoMetDC Antibody (E-6) References:

1. Structure of a human S-adenosylmethionine decarboxylase self-processing ester intermediate and mechanism of putrescine stimulation of processing as revealed by the H243A mutant.  |  Ekstrom, JL., et al. 2001. Biochemistry. 40: 9495-504. PMID: 11583148
2. Mechanism of human S-adenosylmethionine decarboxylase proenzyme processing as revealed by the structure of the S68A mutant.  |  Tolbert, WD., et al. 2003. Biochemistry. 42: 2386-95. PMID: 12600205
3. S-adenosylmethionine decarboxylase degradation by the 26 S proteasome is accelerated by substrate-mediated transamination.  |  Yerlikaya, A. and Stanley, BA. 2004. J Biol Chem. 279: 12469-78. PMID: 14718534
4. HSG cells differentiated by culture on extracellular matrix involves induction of S-adenosylmethione decarboxylase and ornithine decarboxylase.  |  Lam, K., et al. 2005. J Cell Physiol. 203: 353-61. PMID: 15521072
5. S-Adenosylmethionine decarboxylase partially regulates cell growth of HL-60 cells by controlling the intracellular ROS level: Early senescence and sensitization to gamma-radiation.  |  Kim, JS., et al. 2006. Arch Biochem Biophys. 456: 58-70. PMID: 17069747
6. S-adenosyl methionine and DNA methyltransferase-1 mRNA overexpression in psychosis.  |  Guidotti, A., et al. 2007. Neuroreport. 18: 57-60. PMID: 17259861
7. Effects of antisense RNA targeting of ODC and AdoMetDC on the synthesis of polyamine synthesis and cell growth in prostate cancer cells using a prostatic androgen-dependent promoter in adenovirus.  |  Li, W., et al. 2008. Prostate. 68: 1354-61. PMID: 18548481
8. Structural basis for putrescine activation of human S-adenosylmethionine decarboxylase.  |  Bale, S., et al. 2008. Biochemistry. 47: 13404-17. PMID: 19053272
9. Role of the sulfonium center in determining the ligand specificity of human s-adenosylmethionine decarboxylase.  |  Bale, S., et al. 2009. Biochemistry. 48: 6423-30. PMID: 19527050

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-AdoMetDC Antibody (E-6) has 3 citations in a variety of scientific publications.

Primary antibodies like Anti-AdoMetDC Antibody (E-6) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

AdoMetDC Antibody (E-6) Specifications

Alternate Product Name: AdoMetDC

Product Clone Name: E-6

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG₁ kappa light chain

Amino Acids: 5-219

Epitope Target: AdoMetDC

Epitope Species: human

Product Reactivity/Detection: AdoMetDC

Species Reactivity/Detection: mouse, rat, human

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, ELISA

Vialing Information: Each vial contains 200 µg IgG₁ kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 3 citations in various scientific publications

Available Conjugates/Forms for AdoMetDC Antibody (E-6): AC: Agarose, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, FITC: Fluoroscein, HRP: Horseradish Peroxidase, PE: Phycoerythrin