Adipsin Antibody (D10/4): sc-47683

Adipsin Antibody (D10/4) is a mouse monoclonal IgG2a kappa light chain antibody that detects the Adipsin protein of human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and immunohistochemistry. Anti-Adipsin antibody (D10/4) is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. Adipsin, also known as complement factor D, is a serine protease that plays a crucial role in the complement system, specifically in the activation of the alternative pathway. This protein is primarily secreted by adipose tissue, where Adipsin is involved in the regulation of energy homeostasis and inflammation. Adipsin (D10/4) antibody helps researchers study how Adipsin cleaves complement factor B when complexed with activated complement component C3, which is essential for the formation of the C3 convertase enzyme complex. This process is vital for the immune response, as Adipsin helps to enhance opsonization and clearance of pathogens. Interestingly, while Adipsin is highly expressed in healthy adipose tissue, Adipsin levels are significantly reduced in obese individuals, suggesting a potential link between Adipsin expression and metabolic disorders. Understanding the function and regulation of Adipsin can provide insights into obesity and related diseases, making Adipsin monoclonal antibody (D10/4) a valuable tool for researchers studying these critical areas.

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Adipsin Antibody (D10/4) References:

1. Human adipsin is identical to complement factor D and is expressed at high levels in adipose tissue.  |  White, RT., et al. 1992. J Biol Chem. 267: 9210-3. PMID: 1374388
2. Adipsin and an endogenous pathway of complement from adipose cells.  |  Choy, LN., et al. 1992. J Biol Chem. 267: 12736-41. PMID: 1618777
3. Adipsin and complement factor D activity: an immune-related defect in obesity.  |  Rosen, BS., et al. 1989. Science. 244: 1483-7. PMID: 2734615
4. Complement factor D haplodeficiency is associated with a reduced complement activation speed and diminished bacterial killing.  |  Langereis, JD., et al. 2021. Clin Transl Immunology. 10: e1256. PMID: 33841879
5. Complement Factor D (adipsin) Levels Are Elevated in Acquired Partial Lipodystrophy (Barraquer-Simons syndrome).  |  Corvillo, F., et al. 2021. Int J Mol Sci. 22: PMID: 34205507
6. The complement C3-complement factor D-C3a receptor signalling axis regulates cardiac remodelling in right ventricular failure.  |  Ito, S., et al. 2022. Nat Commun. 13: 5409. PMID: 36109509
7. Complement factor D targeting protects endotheliopathy in organoid and monkey models of COVID-19.  |  Kawakami, E., et al. 2023. Cell Stem Cell. 30: 1315-1330.e10. PMID: 37802037
8. Adipsin inhibits Irak2 mitochondrial translocation and improves fatty acid β-oxidation to alleviate diabetic cardiomyopathy.  |  Jiang, MY., et al. 2023. Mil Med Res. 10: 63. PMID: 38072993
9. Combinatorial Inhibition of Complement Factor D and BCL2 for Early-Onset Colorectal Cancer.  |  Rahman, S., et al. 2024. Dis Colon Rectum. 67: 940-950. PMID: 38479005
10. The alternative pathway C3/C5 convertase: chemical basis of factor B activation.  |  Lesavre, PH., et al. 1979. J Immunol. 123: 529-34. PMID: 458145
11. Amino acid sequence of human D of the alternative complement pathway.  |  Niemann, MA., et al. 1984. Biochemistry. 23: 2482-6. PMID: 6383466
12. Independent regulation of adipose tissue-specificity and obesity response of the adipsin promoter in transgenic mice.  |  Platt, KA., et al. 1994. J Biol Chem. 269: 28558-62. PMID: 7961801

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-Adipsin Antibody (D10/4) has 4 citations in a variety of scientific publications.

Primary antibodies like Anti-Adipsin Antibody (D10/4) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

Adipsin Antibody (D10/4) Specifications

Alternate Product Name: CFD

Product Clone Name: D10/4

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG_2a kappa light chain

Amino Acids:

Epitope Target: Adipsin

Epitope Species: human

Product Reactivity/Detection: Adipsin

Species Reactivity/Detection: human

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, IHC(P)

Vialing Information: Each vial contains 200 µg IgG_2a kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 4 citations in various scientific publications

Available Conjugates/Forms for Adipsin Antibody (D10/4): AC: Agarose, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, FITC: Fluoroscein, HRP: Horseradish Peroxidase, PE: Phycoerythrin