ADH8 Antibody (F-9): sc-390387
- ADH8 Antibody (F-9) is a mouse monoclonal IgG1 κ ADH8 antibody provided at 200 µg/ml
- raised against amino acids 20-259 mapping near the N-terminus of ADH8 of human origin
- ADH8 Antibody (F-9) is recommended for detection of ADH8 of human origin by WB, IP, IF, IHC(P) and ELISA
- Anti-ADH8 Antibody (F-9) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
- also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- m-IgG Fc BP-HRP and m-IgG1 BP-HRP are the preferred secondary detection reagents for ADH8 Antibody (F-9) for WB and IHC(P) applications. These reagents are now offered in bundles with ADH8 Antibody (F-9) (see ordering information below).
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ADH8 Antibody (F-9) is a mouse monoclonal IgG1 kappa light chain antibody that detects ADH8 protein of human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), immunohistochemistry with paraffin-embedded sections (IHCP), and enzyme-linked immunosorbent assay (ELISA). ADH8 (F-9) antibody is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. ADH8, also known as ADHFE1 (alcohol dehydrogenase, iron containing, 1) or HOT, is a 467 amino acid enzyme that plays a crucial role in the metabolism of gamma-hydroxybutyrate, converting gamma-hydroxybutyrate to succinic semialdehyde, which is subsequently transformed into succinic acid for energy production in the Krebs cycle. ADH8 operates optimally at a pH of 7.5 and is involved in the reduction of 2-ketoglutarate to D-2-hydroxyglutarate, highlighting ADH8′s importance in various metabolic pathways. Four isoforms of ADH8 exist due to alternative splicing, which may contribute to functional diversity and regulatory mechanisms within the cell.
Alexa Fluor® is a trademark of Molecular Probes Inc., OR., USA
LI-COR® and Odyssey® are registered trademarks of LI-COR Biosciences
ADH8 Antibody (F-9) References:
- Cloning and characterization of a novel human alcohol dehydrogenase gene (ADHFe1). | Deng, Y., et al. 2002. DNA Seq. 13: 301-6. PMID: 12592711
- Crystal structure of the vertebrate NADP(H)-dependent alcohol dehydrogenase (ADH8). | Rosell, A., et al. 2003. J Mol Biol. 330: 75-85. PMID: 12818203
- Two zebrafish alcohol dehydrogenases share common ancestry with mammalian class I, II, IV, and V alcohol dehydrogenase genes but have distinct functional characteristics. | Reimers, MJ., et al. 2004. J Biol Chem. 279: 38303-12. PMID: 15231826
- Kinetic characterization of human hydroxyacid-oxoacid transhydrogenase: relevance to D-2-hydroxyglutaric and gamma-hydroxybutyric acidurias. | Struys, EA., et al. 2005. J Inherit Metab Dis. 28: 921-30. PMID: 16435184
- Identification of the gene encoding hydroxyacid-oxoacid transhydrogenase, an enzyme that metabolizes 4-hydroxybutyrate. | Kardon, T., et al. 2006. FEBS Lett. 580: 2347-50. PMID: 16616524
- Differentiation-dependent expression of Adhfe1 in adipogenesis. | Kim, JY., et al. 2007. Arch Biochem Biophys. 464: 100-11. PMID: 17559793
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
ADH8 Antibody (F-9) | sc-390387 | 200 µg/ml | $316.00 | |||
ADH8 Antibody (F-9): m-IgG Fc BP-HRP Bundle | sc-527627 | 200 µg Ab; 10 µg BP | $354.00 | |||
ADH8 Antibody (F-9): m-IgG1 BP-HRP Bundle | sc-533000 | 200 µg Ab; 20 µg BP | $354.00 | |||
ADH8 Antibody (F-9) AC | sc-390387 AC | 500 µg/ml, 25% agarose | $416.00 | |||
ADH8 Antibody (F-9) HRP | sc-390387 HRP | 200 µg/ml | $316.00 | |||
ADH8 Antibody (F-9) FITC | sc-390387 FITC | 200 µg/ml | $330.00 | |||
ADH8 Antibody (F-9) PE | sc-390387 PE | 200 µg/ml | $343.00 | |||
ADH8 Antibody (F-9) Alexa Fluor® 488 | sc-390387 AF488 | 200 µg/ml | $357.00 | |||
ADH8 Antibody (F-9) Alexa Fluor® 546 | sc-390387 AF546 | 200 µg/ml | $357.00 | |||
ADH8 Antibody (F-9) Alexa Fluor® 594 | sc-390387 AF594 | 200 µg/ml | $357.00 | |||
ADH8 Antibody (F-9) Alexa Fluor® 647 | sc-390387 AF647 | 200 µg/ml | $357.00 | |||
ADH8 Antibody (F-9) Alexa Fluor® 680 | sc-390387 AF680 | 200 µg/ml | $357.00 | |||
ADH8 Antibody (F-9) Alexa Fluor® 790 | sc-390387 AF790 | 200 µg/ml | $357.00 |