Adenovirus-2/5 E1A Antibody (M73): sc-25

Adenovirus-2/5 E1A Antibody (M73) is a mouse monoclonal IgG2a kappa light chain antibody that detects the Adenovirus-2/5 E1A protein of Adenovirus-2 and Adenovirus-5 origin by western blotting (WB), immunoprecipitation (IP), and immunofluorescence (IF). Anti-Adenovirus-2/5 E1A antibody (M73) is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. The E1A protein plays a crucial role in the early stages of adenoviral infection, as E1A is essential for the regulation of viral gene expression and the transformation of host cells. Specifically, E1A interacts with the retinoblastoma (Rb) protein, a key regulator of the cell cycle, which allows the virus to bypass cellular growth controls and promote cell proliferation. This interaction is vital for the adenovirus to effectively hijack the host′s cellular machinery, leading to enhanced viral replication and pathogenesis. Furthermore, E1A′s ability to modulate the transcription of both viral and cellular genes underscores E1A′s importance in the viral life cycle and potential as a target for therapeutic interventions.

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Adenovirus-2/5 E1A Antibody (M73) References:

1. Spliced segments at the 5' terminus of adenovirus 2 late mRNA.  |  Berget, SM., et al. 1977. Proc Natl Acad Sci U S A. 74: 3171-5. PMID: 269380
2. Transfer of functional adenovirus E1A transcription activator proteins into mammalian cells by protoplast fusion.  |  Ferguson, B., et al. 1986. J Biol Chem. 261: 14760-3. PMID: 2945820
3. Tropism and transduction of oncolytic adenovirus 5 vectors in cancer therapy: Focus on fiber chimerism and mosaicism, hexon and pIX.  |  Stepanenko, AA. and Chekhonin, VP. 2018. Virus Res. 257: 40-51. PMID: 30125593
4. Adenovirus 5 Vectors Expressing SARS-CoV-2 Proteins.  |  Bachus, S., et al. 2022. mSphere. 7: e0099821. PMID: 35224978
5. Adenovirus-5 and adenovirus-37 seropositivity in obese patients.  |  Bostan, NG., et al. 2024. Iran J Microbiol. 16: 684-688. PMID: 39534296
6. Molecular cloning and characterization of a cellular phosphoprotein that interacts with a conserved C-terminal domain of adenovirus E1A involved in negative modulation of oncogenic transformation.  |  Schaeper, U., et al. 1995. Proc Natl Acad Sci U S A. 92: 10467-71. PMID: 7479821
7. Nucleotide sequence at the 5' terminus of adenovirus 2 late messenger RNA.  |  Lockard, RE., et al. 1979. J Biol Chem. 254: 587-90. PMID: 762082
8. Evaluation of the respiratory epithelium of normals and individuals with cystic fibrosis for the presence of adenovirus E1a sequences relevant to the use of E1a- adenovirus vectors for gene therapy for the respiratory manifestations of cystic fibrosis.  |  Eissa, NT., et al. 1994. Hum Gene Ther. 5: 1105-14. PMID: 7833370
9. A region in the C-terminus of adenovirus 2/5 E1a protein is required for association with a cellular phosphoprotein and important for the negative modulation of T24-ras mediated transformation, tumorigenesis and metastasis.  |  Boyd, JM., et al. 1993. EMBO J. 12: 469-78. PMID: 8440238
10. A novel C-terminal binding protein (CTBP2) is closely related to CTBP1, an adenovirus E1A-binding protein, and maps to human chromosome 21q21.3.  |  Katsanis, N. and Fisher, EM. 1998. Genomics. 47: 294-9. PMID: 9479502

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-Adenovirus-2/5 E1A Antibody (M73) has 101 citations in a variety of scientific publications.

Primary antibodies for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

Adenovirus-2/5 E1A Antibody (M73) Specifications

Alternate Product Name: Adenovirus-2/5 E1A

Product Clone Name: M73

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG_2a kappa light chain

Amino Acids:

Epitope Target: Adenovirus-2 E1A

Epitope Species: viral

Product Reactivity/Detection: E1A antigens

Species Reactivity/Detection: Adenovirus-2, Adenovirus-5

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF

Vialing Information: Each vial contains 200 µg IgG_2a kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 101 citations in various scientific publications

Available Conjugates/Forms for Adenovirus-2/5 E1A Antibody (M73): AC: Agarose, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, FITC: Fluoroscein, HRP: Horseradish Peroxidase, PE: Phycoerythrin