Date published: 2026-4-12

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Adducin γ Antibody (E-1): sc-74474

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Datasheets
  • Adducin γ Antibody (E-1) is a mouse monoclonal IgG2a κ, cited in 3 publications, provided at 200 µg/ml
  • raised against amino acids 571-630 of Adducin γ of human origin
  • recommended for detection of Adducin γ of mouse, rat and human origin by WB, IP, IF and ELISA
  • m-IgG Fc BP-HRP and m-IgG2a BP-HRP are the preferred secondary detection reagents for Adducin γ Antibody (E-1) for WB applications. These reagents are now offered in bundles with Adducin γ Antibody (E-1) (see ordering information below).
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Adducin γ Antibody (E-1) is a mouse monoclonal IgG2a antibody that detects Adducin γ in mouse, rat, and human samples through applications such as western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). Adducin γ plays a crucial role in organizing the cortical actin cytoskeleton, which maintains cell shape and facilitates cellular processes like migration and division. Adducin γ is primarily localized at the spectrin-actin junction of the plasma membrane, where this protein helps stabilize the cytoskeletal network. Disruptions in Adducin γ activity can lead to cytoskeletal instability, often associated with various pathological conditions, including cancer and neurodegenerative diseases. Adducin γ is expressed ubiquitously, but expression levels vary significantly across different tissues, highlighting Adducin γ′s importance in diverse biological contexts. Through interactions with other cytoskeletal proteins, Adducin γ (E-1) antibody serves as an invaluable tool for researchers studying cellular architecture and function.

For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.

Ordering Information

Product NameCatalog #UNITPriceQtyFAVORITES

Adducin γ Antibody (E-1)

sc-74474
200 µg/ml
$322.00

Adducin γ Antibody (E-1): m-IgG Fc BP-HRP Bundle

sc-539525
200 µg Ab; 10 µg BP
$361.00

Adducin γ Antibody (E-1): m-IgG2a BP-HRP Bundle

sc-546721
200 µg Ab; 10 µg BP
$361.00

What is the recommended fixation method for immunofluorescence staining with Adducin γ (E–1): sc-74474 monoclonal antibody?

Asked by: TinTin
Thank you for your inquiry. We recommend fixing cells for 5 minutes in -10°C methanol and allowing to air dry. The full protocol can be viewed here: https://www.scbt.com/scbt/resources/protocols/immunofluorescence-cell-staining
Answered by: Technical Support
Date published: 2017-02-24
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Rated 5 out of 5 by from Strong positive band obtained in WB in HeLa and KStrong positive band obtained in WB in HeLa and K-562 whole cell lysates. -SCBT QC
Date published: 2015-06-07
Rated 5 out of 5 by from Performed very well in WB in KPerformed very well in WB in K-562 whole cell lysate. -SCBT QC
Date published: 2015-05-15
Rated 5 out of 5 by from Produced exceptional immunofluorescence membraneProduced exceptional immunofluorescence membrane staining in methanol-fixed HeLa cells. -SCBT QC
Date published: 2015-02-20
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Adducin γ Antibody (E-1) is rated 5.0 out of 5 by 3.
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