Anti-ADAR3 Antibody (3.591) is a mouse monoclonal IgG κ ADAR3 antibody, cited in 5 publications, provided at 200 µg/ml
raised against a synthetic peptide corresponding to N terminal amino acids 2-101 of ADAR3 of human origin
Anti-ADAR3 Antibody (3.591) is recommended for detection of native and recombinant ADAR3 of mouse, rat and human origin by WB and IP; non cross-reactive with other members of the ADAR family
Anti-ADAR3 Antibody (3.591) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
Contact our Technical Service Department (or your local Distributor) for more information on how to receive a FREE 10 µg sample of ADAR3 (3.591): sc-73410.
Every item is shipped based on the best shipping method assessed for the temperature requirements of that specific item. Items are grouped and shipped together whenever
possible, and a separate shipping charge will be included for each shipping method required. Shipping charges listed below are from our US warehouses to the Contiguous US,
Alaska, Hawaii, Canada and Puerto Rico. Shipping charges for countries outside the US and Canada will be determined once order has been received
Please note: We can not ship to PO boxes
Express Blue Ice
Express Dry Ice
Animal Health Prescription Item
SHIPPING METHODS & CHARGES
Ships via FedEx Ground to Contiguous US, Alaska, Canada, Monday through Friday. This method is used for less temperature sensitive items such as lab ware and animal
health products, bulky and/or heavy items
Labware ships FedEx Ground free of charge to the contiguous US
ADAR3 Antibody (3.591) is a high quality monoclonal ADAR3 antibody (also designated ADAR3 antibody) suitable for the detection of the ADAR3 protein of mouse, rat and human origin. ADAR3 Antibody (3.591) is available as both the non-conjugated anti-ADAR3 antibody form, as well as multiple conjugated forms of anti-ADAR3 antibody, including agarose, HRP, PE, FITC and multiple Alexa Fluor® conjugates. Editing of RNA alters the nucleotide sequence of a transcript to produce codon changes, which can result in alternative translation patterns from a single pre-mRNA. One type of RNA editing involves tRNA-specific adenosine deaminase, ADAT1, which is responsible for the first step in the processing of eukaryotic tRNAAla transcripts that undergo specific adenosine to inosine modifications. Additionally, members of the double-stranded RNA (dsRNA) adenosine deaminase family of enzymes, ADAR1 and ADAR2, act on double-stranded regions of RNA. dsRNA structures are formed by base pairing of an exonic sequence around the editing site with a complementary sequence in the downstream intron. ADAR family member-mediated editing occurs in the nucleus before splicing removes the respective intron. These enzymes all faciliate the deamination of adenosine to generate inosine, which is then translated as guanosine. ADAR1, ADAR2 and a related brain-specific ADAR family member, ADAR3, contain a central series of double-stranded RNA-binding motifs and a C-terminal catalytic domain. ADAR1 also contains a novel Za-DNA binding domain at the N-terminal region, and when bound to Z-DNA-ADAR1 is substantially less susceptible to proteolytic degradation.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.
Alexa Fluor® is a trademark of Molecular Probes Inc., OR., USA
LI-COR® and Odyssey® are registered trademarks of LI-COR Biosciences
How much sample do you recommend loading on the gel for Western blot detection using ADAR3 (3.591): sc-73410?
Asked by: DefinitelyNotMatt
We recommend loading 40–60 µg whole cell lysate, 10–20 µg nuclear extract, 5-10 µg transfected lysate, or 10–20 ng purified protein per lane. A link to our complete Western blotting protocol is provided here for your reference:
Rated 5 out of
Reliable productsThe ADAR3 we purchased was mouse monoclonal IgG, which is directed against synthetic peptides and is recommended for use in specific applications for the detection of mice by WB and IP
Date published: 2017-02-09
Rated 5 out of
Excellent!This antibody works great in western blot analysis of ADAR3 expression in HeLa whole cell lysate and FLAG-tagged human recombinant ADAR3.
Date published: 2017-01-17
Rated 4 out of
Clean band in western blot using OrigeneClean band in western blot using Origene ADAR3 transfected whole cell lysates. -SCBT QC
Date published: 2015-03-28
Rated 4 out of
Robust bands present in western blot analysisRobust bands present in western blot analysis of ADAR3 expression in HeLa whole cell lysate and FLAG-tagged human recombinant ADAR3. -SCBT QC
To place an order using RMB or to ship to mainland China, please visit www.scbio.cn