ADAR3 Antibody (3.591) recommended for detection of native and recombinant ADAR3 of mouse, rat and human origin by WB and IP

ADAR3 Antibody (3.591): sc-73410

ADAR3 Antibody (3.591) is rated 4.5 out of 5 by 4.
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Datasheets

    • Anti-ADAR3 Antibody (3.591) is a mouse monoclonal IgG κ ADAR3 antibody, cited in 5 publications, provided at 200 µg/ml
    • raised against a synthetic peptide corresponding to N terminal amino acids 2-101 of ADAR3 of human origin
    • Anti-ADAR3 Antibody (3.591) is recommended for detection of native and recombinant ADAR3 of mouse, rat and human origin by WB and IP; non cross-reactive with other members of the ADAR family
    • Anti-ADAR3 Antibody (3.591) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
    • also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
    • also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
    • Contact our Technical Service Department (or your local Distributor) for more information on how to receive a FREE 10 µg sample of ADAR3 (3.591): sc-73410.

How much sample do you recommend loading on the gel for Western blot detection using ADAR3 (3.591): sc-73410?

Asked by: DefinitelyNotMatt
We recommend loading 40–60 µg whole cell lysate, 10–20 µg nuclear extract, 5-10 µg transfected lysate, or 10–20 ng purified protein per lane. A link to our complete Western blotting protocol is provided here for your reference: https://www.scbt.com/scbt/resources/protocols/western-immuno-blotting
Answered by: Technical Support
Date published: 2017-02-24
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Rated 5 out of 5 by from Reliable products The ADAR3 we purchased was mouse monoclonal IgG, which is directed against synthetic peptides and is recommended for use in specific applications for the detection of mice by WB and IP
Date published: 2017-02-09
Rated 5 out of 5 by from Excellent! This antibody works great in western blot analysis of ADAR3 expression in HeLa whole cell lysate and FLAG-tagged human recombinant ADAR3.
Date published: 2017-01-17
Rated 4 out of 5 by from Clean band in western blot using Origene Clean band in western blot using Origene ADAR3 transfected whole cell lysates. -SCBT QC
Date published: 2015-03-28
Rated 4 out of 5 by from Robust bands present in western blot analysis Robust bands present in western blot analysis of ADAR3 expression in HeLa whole cell lysate and FLAG-tagged human recombinant ADAR3. -SCBT QC
Date published: 2013-02-06
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