ADAM5 Antibody (C-6): sc-390150
- ADAM5 Antibody (C-6) is a mouse monoclonal IgG2b κ ADAM5 antibody provided at 200 µg/ml
- raised against amino acids 501-620 mapping near the C-terminus of ADAM5 of rat origin
- ADAM5 Antibody (C-6) is recommended for detection of ADAM5 of mouse and rat origin by WB, IP, IF and ELISA
- Anti-ADAM5 Antibody (C-6) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
- also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- m-IgG Fc BP-HRP and m-IgGκ BP-HRP are the preferred secondary detection reagents for ADAM5 Antibody (C-6) for WB applications. These reagents are now offered in bundles with ADAM5 Antibody (C-6) (see ordering information below).
ADAM5 Antibody (C-6) is a mouse monoclonal IgG2b kappa light chain antibody that detects ADAM5 protein of mouse and rat origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). Anti-ADAM5 antibody (C-6) is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. ADAM5, a member of the disintegrin and metalloproteinase (ADAM) family, plays a crucial role in reproductive biology, particularly in fertilization processes. ADAM5 is primarily expressed on sperm surfaces and remains essential for sperm-egg binding and fusion during successful fertilization. ADAM5 precursor synthesis occurs in the testis, followed by processing during epididymal maturation, highlighting ADAM5′s importance in male fertility. ADAM5 also interacts with other ADAM family members, such as ADAM2 and ADAM3, to facilitate these reproductive processes. Understanding ADAM5′s function and interactions proves vital for elucidating fertilization mechanisms and addressing potential fertility issues.
Alexa Fluor® is a trademark of Molecular Probes Inc., OR., USA
LI-COR® and Odyssey® are registered trademarks of LI-COR Biosciences
ADAM5 Antibody (C-6) References:
- Cloning and chromosomal mapping of mouse ADAM11, ADAM22 and ADAM23. | Sagane, K., et al. 1999. Gene. 236: 79-86. PMID: 10433968
- ADAM 23/MDC3, a human disintegrin that promotes cell adhesion via interaction with the alphavbeta3 integrin through an RGD-independent mechanism. | Cal, S., et al. 2000. Mol Biol Cell. 11: 1457-69. PMID: 10749942
- The ADAM-integrin-tetraspanin complex in fetal and postnatal testicular cords. | Tres, LL. and Kierszenbaum, AL. 2005. Birth Defects Res C Embryo Today. 75: 130-41. PMID: 16035044
- ADAM23, a possible tumor suppressor gene, is frequently silenced in gastric cancers by homozygous deletion or aberrant promoter hypermethylation. | Takada, H., et al. 2005. Oncogene. 24: 8051-60. PMID: 16103878
- Expression and relationship of male reproductive ADAMs in mouse. | Kim, T., et al. 2006. Biol Reprod. 74: 744-50. PMID: 16407499
- Role of integrins, tetraspanins, and ADAM proteins during the development of apoptotic bodies by spermatogenic cells. | Kierszenbaum, AL., et al. 2006. Mol Reprod Dev. 73: 906-17. PMID: 16557522
- Chromosomal assignment of four testis-expressed mouse genes from a new family of transmembrane proteins (ADAMs) involved in cell-cell adhesion and fusion. | Cho, C., et al. 1996. Genomics. 34: 413-7. PMID: 8786143
- A role for the disintegrin domain of cyritestin, a sperm surface protein belonging to the ADAM family, in mouse sperm-egg plasma membrane adhesion and fusion. | Yuan, R., et al. 1997. J Cell Biol. 137: 105-12. PMID: 9105040
- Metalloproteinase-like, disintegrin-like, cysteine-rich proteins MDC2 and MDC3: novel human cellular disintegrins highly expressed in the brain. | Sagane, K., et al. 1998. Biochem J. 334 (Pt 1): 93-8. PMID: 9693107
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
ADAM5 Antibody (C-6) | sc-390150 | 200 µg/ml | $322.00 | |||
ADAM5 Antibody (C-6): m-IgG Fc BP-HRP Bundle | sc-530081 | 200 µg Ab; 10 µg BP | $361.00 | |||
ADAM5 Antibody (C-6): m-IgGκ BP-HRP Bundle | sc-523427 | 200 µg Ab, 40 µg BP | $361.00 | |||
ADAM5 Antibody (C-6) AC | sc-390150 AC | 500 µg/ml, 25% agarose | $424.00 | |||
ADAM5 Antibody (C-6) HRP | sc-390150 HRP | 200 µg/ml | $322.00 | |||
ADAM5 Antibody (C-6) FITC | sc-390150 FITC | 200 µg/ml | $336.00 | |||
ADAM5 Antibody (C-6) PE | sc-390150 PE | 200 µg/ml | $349.00 | |||
ADAM5 Antibody (C-6) Alexa Fluor® 488 | sc-390150 AF488 | 200 µg/ml | $364.00 | |||
ADAM5 Antibody (C-6) Alexa Fluor® 546 | sc-390150 AF546 | 200 µg/ml | $364.00 | |||
ADAM5 Antibody (C-6) Alexa Fluor® 594 | sc-390150 AF594 | 200 µg/ml | $364.00 | |||
ADAM5 Antibody (C-6) Alexa Fluor® 647 | sc-390150 AF647 | 200 µg/ml | $364.00 | |||
ADAM5 Antibody (C-6) Alexa Fluor® 680 | sc-390150 AF680 | 200 µg/ml | $364.00 | |||
ADAM5 Antibody (C-6) Alexa Fluor® 790 | sc-390150 AF790 | 200 µg/ml | $364.00 |