Actin Antibody (MM2/193): sc-81760

Actin Antibody (MM2/193) is a mouse monoclonal IgM kappa light chain antibody, raised against platelet membranes of human origin, and is reactive with mouse, rat, and human species in applications such as western blotting (WB), immunoprecipitation (IP), and immunohistochemistry with paraffin-embedded sections (IHCP). Actin is a fundamental and highly conserved protein that forms microfilaments within the cytoskeleton, playing essential roles in cell motility, shape maintenance, and intracellular transport. In muscle cells, actin interacts directly with myosin to form actomyosin complexes, which drive contraction through the sliding filament mechanism by converting chemical energy into mechanical motion. Beyond muscle function, actin interacts with a broad array of proteins critical for cytoskeletal organization and signaling, including small GTPases such as Rho, Rac, and Cdc42, which regulate actin filament assembly and processes like membrane ruffling and stress fiber formation. Actin-binding proteins like profilin, cofilin, and Arp2/3 also play key roles by modulating filament nucleation, elongation, and branching. Additionally, vinculin and talin connect actin to integrin complexes at focal adhesions, linking the cytoskeleton to the extracellular matrix and influencing cell adhesion and migration. Anti-actin antibody (MM2/193) is invaluable for researchers investigating actin-protein interactions, cytoskeletal regulation, and processes fundamental to cellular dynamics and signal transduction.

The preferred beta Actin antibody is beta Actin Antibody (C4): sc-47778.