ACTH Antibody (B427): sc-57021

ACTH Antibody (B427) is a mouse monoclonal IgG1 kappa light chain antibody specifically designed to detect adrenocorticotropic hormone (ACTH), a peptide derived from the precursor protein pro-opiomelanocortin (POMC). Anti-ACTH antibody (B427) targets amino acids 1-24 at the N-terminus of human ACTH, allowing for the detection of both POMC and the processed active peptide ACTH in mouse, rat, and human samples by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and immunohistochemistry with paraffin-embedded sections (IHCP). ACTH, also known as corticotropin, is a 39-amino-acid peptide hormone produced by the anterior pituitary gland in response to biological stress and stimulates the secretion of cortisol from the adrenal cortex. The structure of ACTH is crucial because its N-terminal region (amino acids 1-24) is essential for binding to the melanocortin 2 receptor (MC2R) on adrenal cells, triggering cortisol release which is vital for stress response, metabolism, and immune function. Understanding the structure-function relationship of ACTH is important for studying disorders like Cushing′s disease, Addison′s disease, and POMC deficiency, which is characterized by adrenal insufficiency, obesity, and altered pigmentation such as red hair. Mutations in the POMC gene can disrupt the processing of ACTH and other peptides like α-MSH and β-endorphin, leading to various endocrine and metabolic abnormalities. ACTH monoclonal antibody (B427) provides researchers with a reliable tool to investigate the expression, processing, and function of ACTH and POMC, facilitating advances in endocrinology and neurobiology.

Alexa Fluor^® is a trademark of Molecular Probes Inc., OR., USA

LI-COR^® and Odyssey^® are registered trademarks of LI-COR Biosciences

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-ACTH Antibody (B427) has 12 citations in a variety of scientific publications.

Primary antibodies like Anti-ACTH Antibody (B427) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

ACTH Antibody (B427) Specifications

Alternate Product Name: ACTH

Product Clone Name: B427

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG₁ kappa light chain

Amino Acids: 1-24

Epitope Target: ACTH

Epitope Species: human

Product Reactivity/Detection: POMC and the processed active peptide ACTH

Species Reactivity/Detection: mouse, rat, human

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, IHC(P)

Vialing Information: Each vial contains 200 µg IgG₁ kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 12 citations in various scientific publications

Available Conjugates/Forms for ACTH Antibody (B427): N/A

The preferred ACTH antibody is ACTH Antibody (2F6): sc-69648.