Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
ACTG1 CRISPR/Cas9 KO Plasmid (m): sc-418967
- Target species: mouse
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- ACTG1 CRISPR/Cas9 Knockout (KO) Plasmid (m) is a pool of plasmids, each encoding Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed for maximum knockout efficiency using sequences derived from the GeCKO v2 library
- gRNA sequences direct Cas9 to induce site-specific double-strand breaks (DSBs) in the ACTG1 genomic locus, resulting in gene knockout through non-homologous end joining (NHEJ)
- The puromycin resistance and RFP genes are flanked by LoxP sites, enabling removal of selection markers via Cre recombinase (Cre Vector: sc-418923) after establishing stable knockout cell lines
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: γ-Actin Antibody (1-17): sc-65638
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
ACTG1 CRISPR/Cas9 KO Plasmid (m) | sc-418967 | 20 µg | $397.00 |
Overview
Actg1 encodes cytoplasmic γ-actin (ACTG1), a highly conserved actin isoform that polymerizes into microfilaments to support cell shape, adhesion, and mechanical integrity. ACTG1 dynamics coordinate actin remodeling with processes such as cytokinesis, cell migration, and vesicle trafficking, and integrate with signaling pathways that regulate cytoskeletal turnover, including Rho family GTPase–dependent networks. In mouse cells, Actg1 contributes to tissue morphogenesis and maintenance of epithelial and mesenchymal architecture by modulating cortical tension and actomyosin organization. Dysregulated actin homeostasis and altered ACTG1 function are relevant to studies of developmental phenotypes, mechanotransduction, and disease-associated cytoskeletal dysfunction affecting cell motility and barrier properties.
ACTG1 CRISPR/Cas9 KO Plasmid (m) is a pool of plasmids designed for targeted disruption of the Actg1 gene in mouse cell lines. Each plasmid co-expresses a unique single guide RNA (sgRNA) targeting a distinct site within the Actg1 together with the Streptococcus pyogenes Cas9 nuclease. The plasmids also encode GFP, allowing fluorescent identification and enrichment of successfully transfected cells by fluorescence microscopy or flow cytometry.
The multi-guide design increases the likelihood of generating insertions or deletions (indels) that disrupt the Actg1 open reading frame following Cas9-mediated double-strand break formation. DNA breaks introduced by the CRISPR/Cas9 system are repaired through endogenous non-homologous end joining (NHEJ) pathways, frequently resulting in frameshift mutations that abolish ACTG1 protein expression.
This CRISPR knockout system enables efficient generation of Actg1-deficient cell models for investigation of ACTG1 signaling, functional genomics studies, cancer biology research, and evaluation of therapeutic responses in human cell lines.
Key Features
- sgRNAs targeting Actg1 exon(s) critical for ACTG1 function
- Co-expression of SpCas9 and sgRNA from a single plasmid for simplified delivery
- GFP reporter for identification of transfected cells
- Pool of plasmids targeting multiple Actg1 genomic sites to improve knockout efficiency
- Compatible with delivery by transfection
Design Variants
CRISPRs +/- HDRs
- gRNAs encoded by ACTG1 CRISPR/Cas9 KO Plasmid (m) and ACTG1 CRISPR/Cas9 KO Plasmid (m2) target distinct sites within the Actg1 locus. One or both targeting designs may be available. See Related Products for availability.
- HDR donor constructs encoded by ACTG1 HDR Plasmid (m) and ACTG1 HDR Plasmid (m2) contain a puromycin resistance cassette and an RFP reporter flanked by Actg1 homology arms to support homology-directed repair at defined Actg1 target sites corresponding to the CRISPR/Cas9 KO designs. HDR donor availability may vary. See Related Products for availability.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.